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Preparation method and application of two-channel environmental estrogen immunosensor

A technology of environmental estrogen and immune sensor, which is applied in the field of preparation of dual-channel environmental estrogen electrochemical immunosensor, can solve the problem of low sensitivity and achieve the effect of improving sensitivity and detection efficiency

Inactive Publication Date: 2014-04-23
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] After searching the existing patent technology, it is found that CN102323323A discloses a preparation method of 17β-estradiol molecularly imprinted membrane electrochemical sensor, and CN101059480A discloses an electrochemical detection method modified by glassy carbon electrodes, and its sensitivity is lower than that of electrochemical immunoassay

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] AFM Fe 3 o 4 and M 2+ -AFMFe 3 o 4 The preparation comprises the following steps:

[0047] (1) Aminated mesoporous ferric oxide (AFFMe 3 o 4 ) preparation

[0048] 1 g FeCl 3 ·6H 2 Dissolve O in 15 mL ethylene glycol, mix well with 3 g NaAc and 10 mL ethylenediamine, stir vigorously for 20 min, put it into a polytetrafluoroethylene reactor, heat at 180 °C for 8 h, take it out, and cool to room temperature , a black solid was obtained, which was washed with ultrapure water until neutral to obtain AFMFe 3 o 4 .

[0049] (2) Aminated mesoporous ferric oxide (M 2+ -AFMFe 3 o 4 ) preparation

[0050] Measure 80 mL of Pb with a concentration of 80 mg / L 2+ solution and 80 mg / L of Cd 2+ The solution was placed in a Erlenmeyer flask, and 8 mg of aminated mesoporous ferric oxide were added respectively, shaken for 1 hour, and the Pb 2+ and Cd 2+ It was fully adsorbed by aminated mesoporous ferric oxide and dried to obtain M 2+ -AFMFe 3 o 4 , namely Pb 2+ -A...

Embodiment 2

[0052] AFM Fe 3 o 4 and M 2+ -AFMFe 3 o 4 The preparation comprises the following steps:

[0053] (1) Aminated mesoporous ferric oxide (AFFMe 3 o 4 ) preparation

[0054] 1 g FeCl 3 ·6H 2 Dissolve O in 17 mL ethylene glycol, mix well with 3 g NaAc and 10 mL ethylenediamine, stir vigorously for 25 min, put it into a polytetrafluoroethylene reactor, heat at 190 °C for 8.5 h, take it out, and cool to room temperature , a black solid was obtained, which was washed with ultrapure water until neutral to obtain AFMFe 3 o 4 .

[0055] (2) Aminated mesoporous ferric oxide (M 2+ -AFMFe 3 o 4 ) preparation

[0056] Measure 90 mL of Pb with a concentration of 90 mg / L respectively 2+ solution and 90 mg / L of Cd 2+ The solution was placed in a Erlenmeyer flask, and 9 mg of aminated mesoporous ferric oxide were added respectively, and shaken for 2 h to make the Pb 2+ and Cd 2+ It was fully adsorbed by aminated mesoporous ferric oxide and dried to obtain M 2+ -AFMFe 3 o 4...

Embodiment 3

[0058] AFM Fe 3 o 4 and M 2+ -AFMFe 3 o 4 The preparation comprises the following steps:

[0059] (1) Aminated mesoporous ferric oxide (AFFMe 3 o 4 ) preparation

[0060] 1 g FeCl 3 ·6H 2 O was dissolved in 18 mL ethylene glycol, mixed evenly with 3 g NaAc and 10 mL ethylenediamine, stirred vigorously for 30 min, put into a polytetrafluoroethylene reactor, heated at 200 °C for 9 h, taken out, and cooled to room temperature , a black solid was obtained, which was washed with ultrapure water until neutral to obtain AFMFe 3 o 4 .

[0061] (2) Aminated mesoporous ferric oxide (M 2+ -AFMFe 3 o 4 ) preparation

[0062] Measure 100 mL of Pb with a concentration of 120 mg / L respectively 2+ solution and 120 mg / L of Cd 2+ The solution was placed in a Erlenmeyer flask, and 10 mg of aminated mesoporous ferric oxide was added respectively, and shaken for 3 h to make the Pb 2+ and Cd 2+ It was fully adsorbed by aminated mesoporous ferric oxide and dried to obtain M 2+ -A...

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Abstract

The invention belongs to the technical field of nanometer functional material, environmental monitoring, immunoassay and bio-sensing and provides a preparation method and an application of a two-channel environmental estrogen electrochemical immunosensor. The electrochemical immunosensor adsorbing metal ions and marked by aminated mesoporous ferroferric oxide is used for simultaneously detecting two environmental estrogens, and the electrochemical immunosensor has high sensitivity and effectively overcomes the defects of the existing environmental estrogen detecting methods, thereby having important scientific significance and application value on environmental monitoring.

Description

technical field [0001] The invention belongs to the technical fields of nanometer functional materials, environmental detection, immune analysis and biosensing, and specifically relates to a preparation method and application of a dual-channel environmental estrogen electrochemical immunosensor. Background technique [0002] A large number of environmentally harmful substances enter the human living environment along with human production activities, causing great harm to human health. Environmental estrogen can affect the human adrenal gland, thyroid gland and other important endocrine glands of the human body, causing them to secrete abnormally, thereby causing dysfunction of the human nervous system and immune system, and seriously affecting human physical and mental health. Environmental estrogen has a great impact on human reproduction, development and autoimmune diseases, which has already attracted widespread attention. [0003] There are many kinds of environmental ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N27/48
Inventor 杜斌魏琴张森李贺辛晓东吴丹马洪敏于海琴张勇
Owner UNIV OF JINAN
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