The invention relates to the technical field of
biochemical engineering, and discloses a process for simultaneously producing maltooligosaccharide and ultrahigh
maltose by a
chromatographic separation method, which comprises the following steps: preparing
starch milk with the concentration of 30-35%, diluting high-temperature alpha-
amylase according to the ratio of 1: 1, adding the diluted high-temperature alpha-
amylase according to the
dry weight of 0.1-0.15% of
starch, adjusting the pH value to 5.5-6.0, spraying for 5-8 minutes at the temperature of 115 + / -2 DEG C, cooling at the temperature of 90-95 DEG C, adding the high-temperature alpha-
amylase according to the
dry weight of the
starch, and adding the high-temperature alpha-amylase according to the ratio of 1: 1 to the starch milk. Carrying out secondary spraying at 135 + / -1 DEG C for 3-5 minutes to inactivate the high-temperature alpha-amylase, so as to obtain liquefied liquid with glucose equivalent of 14 + / -0.5 and DP3 of less than or equal to 3%; cooling the liquefied liquid to 50-60 DEG C, adding beta-amylase accounting for 0.3%-0.5% of the
dry weight of the starch, and adjusting the pH value to 2.0-2.5 by
hydrochloric acid when DP2 is monitored to be 40%-50% by
high performance liquid chromatography so as to inactivate the beta-amylase to obtain final saccharified liquid; and carrying out filter pressing, decoloration and
ion exchange on the saccharified liquid, and synchronously separating the saccharified liquid by using
potassium type cation exchange resin
chromatography to obtain maltooligosaccharide and ultrahigh
maltose. The process realizes synchronous production of the maltooligosaccharide and the ultrahigh
maltose, solves the problem that a traditional process can only realize single production, and improves the starch
utilization rate.