The invention discloses a biological sugaring process by using wheat starch, which comprises the following steps of: (1) size mixing: taking wheat starch milk, adding a sodium carbonate aqueous solution with a certain concentration for regulating pH, adding high-temperature resistant alpha-amylase, hemicellulase and xylanase, and uniformly stirring; (2) jetting and liquefaction: carrying out jetting and liquefaction on the wheat starch milk obtained in the step (1), carrying out flash evaporation and temperature reduction, maintaining temperature till completely liquefying, carrying out vacuum flash evaporation and temperature reduction, and separating; (3) saccharification: regulating the pH of emulsion obtained in the step (2), adding one of or a mixture of more than two of saccharifying enzyme, pentosanase and xylanase, as well as proteolytic enzyme, uniformly stirring, saccharifying, reducing temperature, adding hemicellulase, cellulase and lecithinase, stirring, saccharifying, rising temperature and inactivating enzyme; and (4) filtration. According to the biological sugaring process, the quality of the sugar solution is effectively improved; the conversion rate of the pulverized sugar reaches 106% to 107% (in DS (degree of substitution)); and the DE (dextrose equivalent) value can reach 95% to 98%.