Attached cell culturing container suitable for automatic equipment

Abstract

The invention relates to the field of culturing containers, in particular to an attached cell culturing container suitable for automatic equipment. The container comprises a culturing container bottlebody, a fully air-permeable bottle cap and a cell attaching wall panel, the culturing container bottle body comprises a centrifugal bottle bottom collector, a bottle mouth, an inner cell attaching wall, an oblique slope panel and a cell attaching wall panel, the interior of the centrifugal bottle bottom collector is provided with an oblique slope panel, one end of the centrifugal bottle bottom collector is provided with an inner cell attaching wall, the interior of the inner cell attaching wall comprises a plurality of cell attaching wall panels, and a scaling ruler is arranged at the exterior of the culturing container bottle body. The container has the advantages that the container can be used as a single container in the attached cell culturing process for culturing, centrifugation, sample observation, in-process transferring and the like, liquid bottle opening / closing operation, liquid transferring operation, multi-type container storage and multi-type container conversion in thewhole automatic production process are simplified, and the production efficiency and benefits are effectively improved.

Description

technical field [0001] The invention relates to the field of culture containers, in particular to an adherent cell culture container suitable for automation equipment. Background technique [0002] Immunotherapy and regenerative medicine have entered the stage of large-scale application, which puts forward new requirements for the large-scale production of various types of adherent cells. The traditional manual laboratory production method is far from meeting the needs of large-scale industrial production. Intelligent automation The production system has become a necessary means to solve large-scale adherent cell production. In the automated production process, various containers are required for operations such as cultivation, cell solution transfer, and cell quality inspection. Traditional adherent cell culture containers, in order to adapt to Manual operation generally has the following problems: 1. The container has a single function. The culture container is suitable fo...

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Problems solved by technology

[0002] Immunotherapy and regenerative medicine have entered the stage of large-scale application, which puts forward new requirements for the large-scale production of various types of adherent cells. The traditional manual laboratory production method is far from meeting the needs of large-scale industrial production. Intelligent automation The production system has become a necessary means to solve the large-scale adherent cell production. In the automated production process, various containers are required for operations such as cultivation, cell solution transfer, and cell quality inspection. Traditional adherent cell culture containers, in order to adapt to Manual operation generally has the following problems: 1. The container has a single function. The culture container is suitable for the cultivation process, and the detection container is suitable for the detection process. Risk; 2. Traditional culture containers, such as T75 culture bottles, T175 culture bottles, etc., in order to facilitate manual material storage, can only provide a single plane of the container as a surface for adherent cell culture, the volume of the container is large, and the utilization of unit volume Low efficiency, low utilization rate of culture solution; 3. The design of existing containers is difficult to adapt to automated liquid operation, and it is not convenient for automatic sample collection and detection in the process. In addition to the needs of integrated culture, transfer, and detection operations, it can effectively improve the culture efficiency and increase the yield in a unit space. Therefore, a kind of adherent cell culture container suitable for automation equipment is needed to improve the above problems

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