Animal rabies virus and vaccine and production method thereof

Abstract

The invention discloses a method for producing animal rabies viruses and animal rabies vaccines on a large scale. By using a bioreactor, the animal rabies viruses and the animal rabies vaccines are produced by a cell micro-carrier suspension culturing system on a large scale by the following steps of: inoculating cells for preparing the vaccine into a carrier tank containing culture solution and micro-carriers to attach the cells to the micro-carriers; growing the cells on the micro-carriers until the concentration of culture solution is 5 to 20 times the inoculation concentration under a proper culturing environment; preparing virus suspension from the rabies virus to adsorb the virus suspension to the cells; replacing cell maintenance culture solution to culture the virus under the proper culturing environment; continuously culturing for 3 to 5 days and harvesting the virus solution for the first time, wherein the solution replacement ratio is 50 percent by using a semi-continuous process; continuously culturing for 9 to 11 days and harvesting and replacing the solution once every 24 hours; and mixing the harvested virus solution and the virus solution of the bioreactor, repeatedly performing freeze-thawing twice at the constant temperature of -20 DEG C, inactivating, purifying and adding adjuvants to prepare the rabies vaccine. The method has the advantages of large production scale, high single-batch yield and relatively low production cost.

Description

technical field [0001] The invention relates to a rabies virus and a vaccine for veterinary use and a production method thereof, belonging to the technical field of biological products. Background technique [0002] Rabies is a zoonotic disease caused by rabies virus (Rabies virus, RV) infection with severe symptoms and high fatality rate. The mortality rate is almost 100%. Currently, there is no effective treatment. Wild animals are the main storage hosts of RV. Once people are bitten by such animals carrying rabies virus, they will be infected and diseased, and the case fatality rate is 100%. According to the latest statistics on infectious disease epidemics released by the Ministry of Health, it shows that rabies continues to show signs of danger in recent years. However, there is no specific and effective treatment for rabies, and injection of rabies antigen is the only effective method. [0003] At present, the rabies vaccines on the market are basically subcultured b...

AI Technical Summary

Problems solved by technology

However, there is no specific and effective treatment for rabies, and injection of rabies antigen is the only effective method

[0003] At present, the rabies vaccines on the market are basically subcultured by the spinner bottle process. Large-scale production requires a lot of material and human resources, and the cost is high, and the difference between batches is large, and the quality is unstable.

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