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Streptomyces fradiae as well as application thereof in fermentation of tylosin

A technology of Streptomyces flexneri and tylosin, which is applied in the field of fermentation engineering, can solve the problems of accelerating the D component and restricting the quality of tylosin, so as to reduce the time of heating conversion, avoid excessive accumulation, and improve the product quality. quality effect

Active Publication Date: 2020-05-12
湖北回盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many manufacturers use the heating process to raise the fermentation temperature to 36-39°C in the late stage of fermentation, and raise the temperature for about 20 hours, which can effectively convert the C component into the A component, but because the accumulation of the D component is greatly affected by the temperature, As the temperature rises, the formation of component D will be accelerated, so the content of component D restricts the quality of tylosin. How to convert component C into component A while minimizing the synthesis of component D is the key to tylosin The difficulty of production is also a problem that needs to be solved at present

Method used

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  • Streptomyces fradiae as well as application thereof in fermentation of tylosin
  • Streptomyces fradiae as well as application thereof in fermentation of tylosin
  • Streptomyces fradiae as well as application thereof in fermentation of tylosin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Screening and Identification of Streptomyces fradiae (Streptomyces fradiae) SF-4

[0025] (1) Primary screening of bacterial strains

[0026] 20 strains of tylosin-producing Streptomyces strains screened from soil were initially screened by using Gao's No. 1 medium, which was preserved in the Microbiology Laboratory of Hubei Provincial Veterinary Medicine Research Center. The formula of the culture medium is: cornstarch 2%, potassium nitrate 0.1%, sodium chloride 0.05%, magnesium sulfate heptahydrate 0.05%, dipotassium hydrogen phosphate 0.05%, ferrous sulfate 0.001%, agar 3%, pH7.2 (this In the application documents, unless otherwise specified, 1mol / L sodium hydroxide or 1mol / L hydrochloric acid are used to adjust the pH). Thaw the preserved spores of 20 strains of Streptomyces and spread them on Gao’s No. 1 culture medium plate. After culturing at 30°C for 14 days, observe the colony morphology and the ability of the colony to hydrolyze starch, measure the ...

Embodiment 2

[0038] Embodiment 2 Fermentation Tylosin based on Streptomyces fradiae (Streptomyces fradiae) SF-4

[0039] (1) Preparation of slanted spores

[0040] Take the glycerol tube in which the spores of Streptomyces fradiae SF-4 strain are preserved, and after thawing, use an inoculation needle to dip a small amount of spore liquid and streak it onto the slope of No. The spores were washed with normal saline to prepare a spore suspension, and the concentration of spores in the spore liquid was counted on a hemocytometer.

[0041] (2) Primary seed liquid culture

[0042] Configure primary seed medium: peanut cake powder 5g / L, yeast extract powder 3g / L, corn steep liquor 7g / L, soybean oil 10g / L, calcium carbonate 3g / L, pH7.2. Dispense into 250ml shaker flasks, 50ml per bottle, and sterilize at 121°C for 20min for later use. Take the prepared spore suspension, and the final concentration of the spores in the primary seed liquid is 1.1×10 according to the inoculum size. 6 cells / ml, ...

Embodiment 3

[0050] Embodiment 3 fermentation tylosin based on Streptomyces fradiae (Streptomyces fradiae) SF-4

[0051] (1) Preparation of slanted spores

[0052] Take the glycerol tube that contains the spores of Streptomyces fradiae SF-4 strain. After thawing, use an inoculation needle to dip a small amount of spore liquid and streak it to the inclined surface of the test tube. Wash the spores, prepare a spore suspension, and count the spore concentration in the spore liquid with a hemocytometer.

[0053] (2) Primary seed liquid culture

[0054] Configure primary seed medium: peanut cake powder 8g / L, yeast extract powder 3g / L, corn steep liquor 6g / L, soybean oil 12g / L, calcium carbonate 2g / L, pH7.2. Dispense into 250ml shaker flasks, 50ml per bottle, and sterilize at 121°C for 20min for later use. Take the prepared spore suspension, according to the inoculum size, the final concentration of spores in the seed liquid is 1.4×10 6 cells / ml, inoculated into the seed bottle, 30°C, 220 r / ...

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Abstract

The invention belongs to the technical field of fermentation engineering, and particularly relates to streptomyces fradiae SF-4 as well as an application thereof in fermentation of tylosin. The streptomyces fradiae SF-4 was deposited in the China typical culture preservation center on October 9, 2019, with the deposit number of CCTCC NO: M2019791. The invention further discloses a method for the fermentation of the tylosin by the strain, an obtained tylosin fermentation solution can effectively control the synthesis of component D by use of the method, and the quality of the tylosin is improved.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a strain of Streptomyces freundii and its application in tylosin fermentation. Background technique [0002] Tylosin is a sixteen-membered macrolide antibiotic synthesized by Streptomyces freundii. It can selectively inhibit protein synthesis and effectively prevent respiratory and digestive tract infections in livestock and poultry. Its preparations are now widely used in livestock and poultry farming. In addition, tylosin is the precursor raw material of new macrolide antibiotics such as tilmicosin, tyvanectin and tedirocin, so tylosin has a wide range of applications in animal health care and has a wide range of applications. market expectation. [0003] Tylosin is composed of four components: Tylosin A, B, C and D, among which Tylosin A is the main component and has the highest activity in organisms. And as the precursor raw material of tilmico...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/62C12R1/54
CPCC12N1/20C12P19/62C12R2001/54C12N1/205Y02A40/70
Inventor 张卫元戴军康远军余晨刘杨凌云龚正勇陈翠兰
Owner 湖北回盛生物科技有限公司
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