37 results about "Myceliophthora thermophila" patented technology

Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and / or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate aminotransferase, glutamic acid-aspartate transporter, C4-dicarboxylic acid transporter, pyruvate carboxylase and malate dehydrogenase, glucose transporter; the expression product of the negative regulatory gene comprises succinyl-CoA synthase, and malic acid-alpha ketoglutarate transporter, and the original strain comprises myceliophthora thermophila, thielavia terrestris, aspergillus, and rhizopus.

The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.

The invention discloses a lytic polysaccharide monooxygenase modified by site-directed mutation, its construction method and application, and belongs to the field of genetic engineering. The enzyme activity provides a degradative function in the degradation of crystalline polysaccharides of cellulose. The lytic polysaccharide monooxygenase mutant of the present invention is based on the amino acid sequence of the lytic polysaccharide monooxygenase of wild-type Myceliophthora thermophila, and the 34th position is mutated from Arg to Leu. The optimal temperature of the wild enzyme and the wild enzyme is both 85°C, and the specific activity of the mutant enzyme is about 2 times higher than that of the wild enzyme, and when the pH is greater than 5.5, the specific activity of the mutant enzyme is about 2 times higher than that of the wild enzyme. When microcrystalline cellulose was used as the substrate, the glucose content produced by the synergistic reaction of cellulase and mutant enzyme was significantly increased by 48.5% compared with the substrate degraded by cellulase alone. It can be seen that the mutant enzyme significantly improved the enzyme activity, which can Lower economic costs for industrial applications.

The invention discloses a myceliophthora thermophila exoglucosidase mutant and application thereof. A protein of the invention is named as CBH1-DM and is one of the following proteins (a) and (b): (a)a protein composed of amino acid sequences shown in SEQ ID NO.2 in a sequence table; and (b) a fusion protein obtained by connecting a label at an N terminal or / and C terminal of (a). Compared with awild type, the myceliophthora thermophila exoglucosidase mutant disclosed by the invention has the advantages that enzymatic specific activity is improved by 2.1 times, catalysis efficiency is improved by 8.6 times, and thermal stability is improved by 2 times, so that the myceliophthora thermophila exoglucosidase mutant has a better industrial application prospect.