37 results about "Myceliophthora thermophila" patented technology

The invention discloses an organic material decomposing agent, which comprises the following components in percentage by weight: 10-50 parts of Streptomyces fradiae CGMCC No.5133 microbial inoculum, 5-20 parts of Myceliophthora thermophila CGMCC No. 5134 microbial inoculum, 0-20 parts of Trichoderma viride microbial inoculum, 0-20 parts of Bacillus subtilis microbial inoculum and 0-20 parts of microzyme inoculum. The invention provides the organic material decomposing agent with auxin accumulation function which utilizes the quick accumulation of auxin generated by the Streptomyces fradiae in the medium temperature stage and the matching of high temperature function of the Myceliophthora thermophila in the anaphase to realize quick treatment and final resource utilization of organic materials, and treated organic solid wastes are fundamentally transformed, embody good performances in improving soil, promoting growth and inhibiting soil-borne diseases and meanwhile are environment-friendly and sanitary.

The present invention relates to cellobiohydrolase variants having improved thermostability and/or thermoactivity in comparison to wild-type Myceliophthora thermophila CBH2b.

The invention discloses site-directed mutation modified lytic polysaccharide monooxygenase as well as a construction method and application thereof, and belongs to the field of gene engineering. The activity of the site-directed mutation modified lytic polysaccharide monooxygenase provides a degrading function in degradation of crystalline polysaccharide of cellulose. A lytic polysaccharide monooxygenase mutant provided by the invention is formed by mutating Arg into Leu in the 34th site on the basis of the amino acid sequence of lytic polysaccharide monooxygenase of wild type myceliophthora thermophila, wherein the optimum temperature of the mutant enzyme and the wild enzyme is 85 DEG C; the specific activity of the mutant enzyme is significantly increased by about two times compared withthat of the wild enzyme; and when pH is more than 5.5, the specific activity of the mutant enzyme is significantly about two times higher than that of the wild enzyme. When microcrystalline celluloseserves as a substrate, the content of glucose generated through synergistic reaction of cellulase and the mutant enzyme is significantly increased by 48.5 percent compared with that in the method ofdegrading the substrate singly by the celluase, so it is observed that the mutant enzyme significantly improves the enzyme activity and the economic cost of industrial application can be reduced.

The invention discloses Myceliophthora thermophilia strain and an application thereof in the aspect of producing keratinase, the preservation number of Myceliophthora thermophilia strain GZUIFR-H94.1 is CCTCC NO: M 209140. The method for producing keratinase by the Myceliophthora thermophilia strain GZUIFR-H94.1 takes 20.10g/L of chicken feather powder as carbon source, nitrogen source and inductor, takes 0.98g/L of urea as supplement nitrogen source, and takes 5g/L of NaCl, 6g/L of K2HPO4 and 1g/L of KH2PO4 as enzyme-producing culture medium, wherein, the enzyme-producing temperature is 38 DEG C, the initial pH value is 7.9, and the above materials are continuously cultured for 6 days. The strain can grow fast, is thermophilic, and has stronger keratinase producing ability.

The invention belongs to the technical field of microorganisms and specifically discloses myceliophthora thermophila as well as a solid-state fermentation method and application thereof. An inventor obtains the myceliophthora thermophila MT1810 (CCTCC (China Center for Type Culture Collection) NO: M2018705) with stable heredity through separating, purifying, carrying out ion beam and cobalt mutagenesis and screening. After a strain is cultured on an optimized fermentation culture medium, the enzyme activity of xylanase can reach 1800 U/g or more and the viable count is greater than 20*10<8> CFU/g. The strain, aspergillus niger, trichoderma reesei, bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae are compounded according to a certain ratio to prepare a fiber material composting bacterium agent; and when the fiber material composting bacterium agent is used in a corn straw composting experiment, the composting time can be shortened and the content of humus is improved. When the composting bacterium agent is used in a composting process of fiber materials, the composting effect can be enhanced; the quality of products is improved; and the composting bacterium agent has a wide application prospect.

The present invention relates to a host cell, preferably Myceliophthora thermophila cell, which expresses recombinant enzymes with beta-xylosidase activity, an enzymatic composition comprising said cell and/or the recombinant enzyme with beta-xylosidase activity expressed by said cell, the use of this host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or the composition for the degradation of biomass and a method of producing bioproducts, preferably bioethanol, which comprises the use of said host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or said composition.

The invention discloses a method for preparing a polysaccharide cracking monooxygenase LPMO 9D coding gene derived from myceliophthora thermophila and an enzyme thereof, and an application thereof. The polysaccharide cracking monooxygenase LPMO 9D of the present invention is derived from Thermomyxomyces thermophila ATCC 42464. The invention also provides a method for preparing the novel polysaccharide cracking monooxygenase, which is characterized in that the gene of the new polysaccharide cracking monooxygenase is cloned into a Pichia pastoris expression vector by using a genetic engineeringmethod to obtain a recombinant strain of Pichia pastoris capable of performing heterogenous expressing of the enzyme, and the polysaccharide cracking monooxygenase LPMO 9D prepared by heterologous expression of the strain can degrade PASC (phosphorylated microcrystalline cellulose) to produce cellooligosaccharide and cellooligosaccharide. The polysaccharide cracking monooxygenase LPMO 9D providedby the invention can be widely used in the fields of chemical industry, agriculture, feed addition and medicine, and has great production potential and economic value.

The invention discloses a novel myceliophthora thermophila strain (CLIMAX-VMT). The preservation number of the novel myceliophthora thermophila strain is CGMCC No.5134, the preservation date is August 12th, 2011, and the preservation unit is General Microbiology Center of China Microbial Strain Preservation Management Committee. The invention also discloses a microbial inoculum containing the strain. The novel myceliophthora thermophila strain (CLIMAX-VMT) provided by the invention has very obvious fast fermentation temperature rise and cellulose decomposition performance and is innocuous and harmless to people and livestock. When the strain is used for fermenting organic solid waste materials, the fast temperature rise in 24h can be reached, and the temperature is raised to a value higher than 60 DEG C in 48h and can be maintained for more than 5 days. The strain does not have the obvious antagonism action on most low-temperature germs, i.e. the universality and the matching with other strains of the microbial inoculum are very good.

The invention discloses a thermophilic fungus glucoamylase MhGlaA having an amino acid sequence as shown in SEQID NO.1, a gene for coding the thermophilic fungus glucoamylase MhGlaA, an expression boxmediated with FMDV 2A peptide and containing the gene and a GFP gene, and a recombinant expression carrier, and further discloses a method for increasing yield of glucoamylase through myceliophthorathermophila, and an application of the glucoamylase to hydrolyzed starch. The glucoamylase provided by the invention is wide in pH value application range, good in heat stability and notable in hydrolyzing effects on starch; particularly, the yield of the glucoamylase produced through an obtained optimal myceliophthora thermophila recombinant strain is increased by about 12.5 times than that of the glucoamylase produced through a wild strain, the vitality of the glucoamylase produced through the optimal myceliophthora thermophila recombinant strain is improved by about 8.5 times than that of the glucoamylase produced through the wild strain, and the starch hydrolyzing effects of the fermentation liquid of the glucoamylase produced through the optimal myceliophthora thermophila recombinantstrain are notably better than those of the fermentation liquid of the glucoamylase produced through the wild strain, and therefore, the thermophilic fungus glucoamylase MhGlaA has higher applicationvalue.

Recombinant expression of influenza vims surface proteins in the fungus Myceliophthora thermophila strain Cl is provided. The recombinant proteins are for use in influenza vaccine compositions.

The invention discloses a myceliophthora thermophila ferment as well as a preparation method and an application thereof. The invention provides a method for preparing the ferment, wherein the method comprises the following steps: inoculating the myceliophthora thermophila ACCC No. 30572 into a fermenting culture medium, vibrating and culturing for 5-9 days under the condition of 40-55 DEC and 100-300rpm; the fermenting culture medium is prepared by the method comprising the following steps: mixing a carbon source, a nitrogen source, ammonium sulfate, monopotassium phosphate, calcium carbonate, magnesium sulfate, glycerol and tween-80 with water, thus obtaining the fermenting culture medium, wherein the concentration of the carbon source is 25-60g/L, the concentration of the nitrogen source is 10-40g/L, the concentration of the ammonium sulfate is 1-10g/l, the concentration of the monopotassium phosphate is 1-10g/L, the concentration of the calcium carbonate is 1-4g/L, the concentration of the magnesium sulfate is 0.5-4g/L, the concentration of the glycerol is 1-4g/L, and the concentration of the tween-80 is 0.5-3g/L. The ferment provided by the invention has the activity of cellulose, has excellent temperature stability and pH (potential of hydrogen) stability, namely, has higher enzyme activity under a high-temperature condition, and has higher enzyme activity within a wider pH scope.

The invention relates to a method for producing superoxide dismutase (SOD) by using myceliophthora thermophila and belongs to the technical field of bioengineering. According to the method, SOD is produced from myceliophthora thermophila under the condition of liquid-state fermentation and is an ectoenzyme. The yield of heat-resistant SOD can reach 2000U/ml to the maximum, so that the method has a good industrial application prospect.

Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate aminotransferase, glutamic acid-aspartate transporter, C4-dicarboxylic acid transporter, pyruvate carboxylase and malate dehydrogenase, glucose transporter; the expression product of the negative regulatory gene comprises succinyl-CoA synthase, and malic acid-alpha ketoglutarate transporter, and the original strain comprises myceliophthora thermophila, thielavia terrestris, aspergillus, and rhizopus.

The present invention relates to a Myceliophthora thermophila cell, which expresses a nucleotide sequence that codifies a recombinant beta-xylosidase enzyme comprising an amino-acid sequence having at least 70% identity with SEQ ID NO: 1, an enzymatic composition comprising said cell and/or the recombinant enzyme with beta-xylosidase activity expressed by said cell, the use of this host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or the composition for the degradation of biomass, and a method of producing biological products, preferably bioethanol, comprising the use of said host cell, the recombinant enzyme with the beta-xylosidase activity expressed by said cell or said composition.

The invention provides a method for producing a multifunctional distiller's grain feed, which mainly comprises the following steps: pretreating raw material vinasse, and inoculating microbial strainsto ferment by using the pretreated vinasse as a fermentation substrate, thereby obtaining the multifunctional distilled spirit vinasse feed, wherein the microbial strains consist of saccharomyces cerevisiae, candida utilis, lactobacillus plantarum, bacillus subtilis, trichoderma koningii, aspergillus niger CICC 2103, aspergillus niger CICC 2041 and Myceliophthora thermophila. Various functional microorganisms are mixed to ferment the vinasse, so that the problems of insufficient nutrition and single function of the vinasse feed can be solved, and the vinasse feed has the functions of promotinganimal growth, improving animal immunity, improving feed palatability and the like, and can also enrich animal intestinal beneficial flora.

The present invention relates to a method of producing a recombinant polypeptide in a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 1 (CLR1) and to express the recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR1 and to the use of this filamentous fungus in the production of a recombinant polypeptide.

The invention relates to novel and heat-stable heat-resistant dual-function glucoside hydrolase MtCel2 of Myceliophthora thermophila and an encoding sequence and application thereof. A DUF4360 superfamily glucoside hydrolase gene MtCel2 is obtained from the Myceliophthora thermophila through a RT-PCR method and is cloned and inserted into a pichia pastoris integrative expression vector pPIC9K; then the obtained glucoside hydrolase gene MtCel2 expression vector pPIC9K/MtCel2 is transferred into pichia pastoris GS115, engineering saccharomycetes GS-MT-Cel2 expressing the glucoside hydrolase gene MtCel2 screened out, and the glucoside hydrolase gene MtCel2 generated by expression of the engineering saccharomycetes GS-MT-Cel2 has high endonuclease hydrolase activity to hydrolase and xylan. MtCel2 has good heat stability and high cellulose hydrolysis capacity, can be widely applied to the field of fiber waste materials and other industrial fields and has significant economic value and social value.