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Novel myceliophthora thermophila strain and application thereof

A technology of Myceliophthora thermophila and new strains, applied in the direction of fungi, organic fertilizers, microorganisms, etc., can solve the problems of farmland ecological environment, soil physical and chemical properties, soil microbial flora destruction, insufficient supply of organic fertilizers, and affecting the quality of agricultural products, etc. , to achieve good biological activity, significant fermentation temperature rise and cellulolytic performance, rapid fermentation temperature rise and cellulolytic performance effects

Active Publication Date: 2012-02-15
BEIJING JINGGENG TIANXIA AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term application of chemical fertilizers, the supply of organic fertilizers is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical properties and soil microbial flora, which affects the quality of agricultural products to a certain extent.

Method used

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  • Novel myceliophthora thermophila strain and application thereof
  • Novel myceliophthora thermophila strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Identification of Myceliophthora thermophila CLIMAX-VMT

[0025] Myceliophthora thermophila CLIMAX-VMT of the present invention has following colony form and form:

[0026] The colony grows fast on the malt juice agar medium (MEA), cultured in the dark at 40°C for 5 days, the colony diameter is 40-50mm; the colony is white at the beginning, and then the white color of the colony turns grayish pink to grayish brown, and the texture is flocculent; the back is gray Brown, without water-soluble pigment.

[0027] Mycelium is colorless, multi-separated, 2-5 μm wide. Conidiophores are unspecialized, sporulating on short stalks and urn-shaped protrusions on the top or side of mycelium. Conidia terminal or lateral, solitary, rarely chain, elliptic or nearly rod-shaped, base truncate, colorless to light brown, smooth or rough, 4.5-7.5×2.8-4.5μm.

[0028] The above characteristics are consistent with the characteristics of Myceliophthora thermophila.

[0029] The seq...

Embodiment 2

[0031] The preparation of embodiment 2 Myceliophthora thermophila CLIMAX-VMT original bacterial agent

[0032] The invention adopts a liquid-solid combined fermentation culture process to cultivate the myceliophthora thermophila fungus agent, and through the first liquid multiplication culture and then inserted into the solid culture multiplication culture, the formed fungus agent has strong biological enzyme activity, and the specific steps include:

[0033] 1) Seed preparation and liquid proliferation culture: first activate the freeze-dried tube containing Myceliophthora thermophila CLIMAX-VMT, the specific method is to first insert the bacteria in the freeze-dried tube into PDA culture on a sterile operating table cultured at 42°C for 5 days on the slant of the substrate; after obtaining healthy colonies, they were inserted into nutrient agar medium and cultured at 42°C for 5 days, and then the healthy strains were selected and inserted into sterilized 10 parts of sucrose ...

Embodiment 3

[0041] Embodiment 3 Compatibility of Myceliophthora thermophila CLIMAX-VMT and other low-temperature bacteria

[0042] Myceliophthora thermophila CLIMAX-VMT inoculum of the present invention: Bacillus subtilis inoculum: Saccharomyces cerevisiae inoculum: zeolite powder = 1: 1: 1: 1 is mixed, and its respective viable counts are as described in the table below . After comparing the data of various bacteria mixed with zeolite powder at a ratio of 1:3, it was tested on the 30th day that there was no significant loss in the number of bacteria in each bacteria, indicating that Myceliophthora thermophila has very good multi-species adaptability (Table 1).

[0043] Table 1 Compatibility of Myceliophthora thermophila CLIMAX-VMT with other low-temperature bacteria

[0044]

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Abstract

The invention discloses a novel myceliophthora thermophila strain (CLIMAX-VMT). The preservation number of the novel myceliophthora thermophila strain is CGMCC No.5134, the preservation date is August 12th, 2011, and the preservation unit is General Microbiology Center of China Microbial Strain Preservation Management Committee. The invention also discloses a microbial inoculum containing the strain. The novel myceliophthora thermophila strain (CLIMAX-VMT) provided by the invention has very obvious fast fermentation temperature rise and cellulose decomposition performance and is innocuous and harmless to people and livestock. When the strain is used for fermenting organic solid waste materials, the fast temperature rise in 24h can be reached, and the temperature is raised to a value higher than 60 DEG C in 48h and can be maintained for more than 5 days. The strain does not have the obvious antagonism action on most low-temperature germs, i.e. the universality and the matching with other strains of the microbial inoculum are very good.

Description

technical field [0001] The invention belongs to the field of soil fertilizer, and in particular relates to an agricultural microbial preparation and its application. Background technique [0002] Agricultural production requires soil to provide nutrients for crop growth and a living environment. The application of chemical fertilizers has played a key role in increasing agricultural incomes for many years. However, due to the long-term application of chemical fertilizers, the supply of organic fertilizers is insufficient, and the proportion of various nutrients is out of balance, resulting in varying degrees of damage to the farmland ecological environment, soil physical and chemical properties, and soil microbial flora, affecting the quality of agricultural products to a certain extent. Therefore, chemical fertilizer pollution has become a major public hazard in the world today. The current status of soil resources in my country urgently needs to supplement soil organic ma...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/645
Inventor 刘澎
Owner BEIJING JINGGENG TIANXIA AGRI TECH
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