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Functional organic material decomposing agent and preparation method thereof

A decomposing agent and bacterial agent technology, applied in the field of agricultural microbial preparations, can solve problems such as undiscovered, achieve long shelf life, good biological activity, and soil improvement effects

Active Publication Date: 2012-10-24
廊坊市精耕天下农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The products with the function of decomposing organic matter currently on the market are mainly characterized by the decomposing of organic matter. The main technical process of its products is to use solid adsorbents for adsorption after liquid fermentation. After the material is decomposed and fermented, the product can increase the production of Streptomyces freundii and Myceliophthora thermophila through a liquid-solid joint fermentation process.

Method used

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  • Functional organic material decomposing agent and preparation method thereof
  • Functional organic material decomposing agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 Streptomyces flexneri CGMCC No.5133 original bacterial agent

[0032] The present invention adopts the liquid-solid combined fermentation culture process to carry out the culture of Streptomyces freundii CLIMAX-VSF, through the first liquid multiplication culture and then inserting into the solid culture multiplication culture, the specific steps include:

[0033] 1) Seed preparation and liquid multiplication culture: first activate the freeze-dried tube containing Streptomyces flexneri CLIMAX-VSF. The specific method is to first insert it into the slant of Gao’s No. 1 medium and cultivate it at 30±2°C for 15 days; after obtaining healthy colonies, insert it into PDA medium and cultivate it at 30°C for 5 days, and then select healthy colonies. The strains were inserted into a sterilized liquid medium consisting of 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of potassium dihyd...

Embodiment 2

[0036] The preparation of embodiment 2 Myceliophthora thermophila CGMCC No.5134 original bacterial agent

[0037] 1) Seed preparation and liquid proliferation culture: first activate the freeze-dried tube containing Myceliophthora thermophila CGMCC No.5134, the specific method is to first connect the bacteria in the freeze-dried tube to the PDA on the aseptic operating table Cultivate at 42°C for 5 days on the slant of the medium; after obtaining healthy colonies, insert them into nutrient agar medium and culture at 42°C for 5 days, then select healthy strains for development and insert them into sterilized sucrose 10 1 part, 5 parts of sodium chloride, 10 parts of beef extract, 5 parts of calcium chloride, 0.5 part of potassium dihydrogen phosphate, 0.01 part of ferric chloride, 2 parts of yeast extract, and 1000 parts of water. 150r / min, cultured at 42°C for 3 days to obtain Myceliophthora thermophila seed liquid.

[0038] 2) Solid proliferation culture: insert the Myceliop...

Embodiment 3

[0040] The preparation of embodiment 3 Trichoderma viridans ACCC 30169 original bacterial agent

[0041]1) Seed preparation and liquid proliferation culture: first activate the freeze-dried tube containing Trichoderma viride ACCC30169 (purchased from the China Agricultural Microorganism Culture Collection Management Center), the specific method is to first put the freeze-dried tube in the aseptic operation table The strains were inserted into the slant of PDA medium and cultured at 30±2°C for 5 days; after obtaining healthy colonies, they were inserted into nutrient agar medium, cultured at 30±2°C for 5 days, and then selected The healthy strains were inserted into the sterilized 10 parts of sucrose, 10 parts of sweet potato juice, 5 parts of sodium chloride, 10 parts of peptone, 5 parts of magnesium sulfate, 0.5 parts of potassium dihydrogen phosphate, 3 parts of ammonium sulfate, and 1000 parts of water. In a liquid medium composed of parts, and cultivated at 150r / min, 30±2°...

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Abstract

The invention discloses an organic material decomposing agent, which comprises the following components in percentage by weight: 10-50 parts of Streptomyces fradiae CGMCC No.5133 microbial inoculum, 5-20 parts of Myceliophthora thermophila CGMCC No. 5134 microbial inoculum, 0-20 parts of Trichoderma viride microbial inoculum, 0-20 parts of Bacillus subtilis microbial inoculum and 0-20 parts of microzyme inoculum. The invention provides the organic material decomposing agent with auxin accumulation function which utilizes the quick accumulation of auxin generated by the Streptomyces fradiae inthe medium temperature stage and the matching of high temperature function of the Myceliophthora thermophila in the anaphase to realize quick treatment and final resource utilization of organic materials, and treated organic solid wastes are fundamentally transformed, embody good performances in improving soil, promoting growth and inhibiting soil-borne diseases and meanwhile are environment-friendly and sanitary.

Description

technical field [0001] The invention belongs to the field of soil fertilizer, and in particular relates to an agricultural microbial preparation and its application. Background technique [0002] Agricultural production requires soil to provide nutrients for crop growth and a living environment. The application of chemical fertilizers has played a key role in increasing agricultural incomes for many years. However, due to the long-term application of chemical fertilizers, the supply of organic fertilizers is insufficient, and the proportion of various nutrients is out of balance, resulting in varying degrees of damage to the farmland ecological environment, soil physical and chemical properties, and soil microbial flora, affecting the quality of agricultural products to a certain extent. Therefore, chemical fertilizer pollution has become a major public hazard in the world today. The current status of soil resources in my country urgently needs to supplement soil organic ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/16C12N1/18C05F11/08C12R1/54C12R1/645C12R1/885C12R1/125C12R1/72C12R1/865
Inventor 刘澎
Owner 廊坊市精耕天下农业科技有限公司
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