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Method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores

A technology of Phytophthora nicotianae and zoospores, which is applied in the methods of using spores, biochemical equipment and methods, microorganisms, etc., can solve the problems of bacterial contamination and difficulty in obtaining plant materials, and achieve the effect of shortening the induction period

Active Publication Date: 2013-04-17
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method takes less time, but is prone to bacterial contamination and plant material is more difficult to obtain

Method used

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  • Method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores
  • Method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores
  • Method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Phytophthora tabacum standard strain produces sporangia and zoospores

[0036] (1) Inoculate Phytophthora nicotianae (strain number: GIM3.567, purchased from the Guangdong Microbial Culture Collection Center) on the PDA medium on a clean bench, and incubate in the dark at 27°C for 2 days.

[0037] (2) On the ultra-clean workbench, use a puncher with a diameter of 0.5 cm to punch holes at the edge of the colony, take a piece of mycelium and place it in a sterile petri dish with a diameter of 6 cm, and add a volume concentration of 20% 5mL of compound vegetable and vegetable juice of Betsy’s wild vegetables, cultured in the dark at 27°C for 2 days; then pour out the juice, replace it with 5mL of sterile compound fertilizer solution with a mass concentration of 0.1%, and place it in a light incubator with a white fluorescent lamp with a light intensity of 600Lux (Guangdong Shaoguan Taihong Medical Devices Co., Ltd. (model: LRH-250-G), cultivated under continuous ...

Embodiment 2

[0041] Embodiment 2 induces the Phytophthora nicotiana strain isolated in the fresh loofah blight sample to produce sporangia and zoospores

[0042] (1) Take a freshly-onset loofah blight specimen, and use scissors to cut out a tissue block with a size of about 2mm×5mm at the junction of disease and health.

[0043] (2) On the ultra-clean workbench, soak the tissue block in 75% alcohol for 30s, then soak and rinse in 5% sodium hypochlorite solution for 1min; then change and wash with sterilized water for 3 times. After the water was blotted dry on sterile filter paper, it was transferred to a PDA medium plate and incubated in the dark at 27°C for 4 days.

[0044] (3) After the colony grows, pick the edge mycelium block with white colony color on the ultra-clean workbench, and purify the mycelium to obtain Phytophthora nicotianae.

[0045] (4) On the ultra-clean workbench, put the purified strain in the Betsy wild vegetable compound vegetable juice with a volume concentration ...

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Abstract

The invention belongs to the technical field of plant protection, and discloses a method of inducing phytophthora nicotianae breda de haan bacteria to generate sporangiums and release zoospores. The method comprises the following steps: activating the phytophthora nicotianae breda de haan bacteria, preparing the induction liquid generating sporangiums and inducing to generate sporangiums and release zoospores. According to the invention, the used induction liquid is the Bache wild vegetable composite fruit and vegetable juice with the volume concentration of 15-25 percent and the compound fertilizer solution with the mass concentration of 0.05-0.20 percent, and when the phytophthora nicotianae breda de haan is induced to generate the sporangiums, the continuous illumination culture is performed under the white fluorescent lamp. The method provided by the invention is simple, convenient, rapid and pollution-free, and can be used for the researches on virulence determination of the phytophthora nicotianae breda de haan bacteria, the biological activity assay of the phytophthora nicotianae breda de haan bacteria by the sterilizing agent, the evaluation of disease resistance of an epidemic disease by the luffa varieties and the like.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and relates to a method for inducing Phytophthora tabacum to produce sporangia and release zoospores. Background technique [0002] Phytophthora nicotianae Breda de Haan is the pathogenic fungus that causes the blight of loofah, and it does serious damage to the production of loofah. Under the condition of suitable climate, the epidemic can break out in a short period of time, causing great economic losses. Therefore, it is often necessary to obtain a large number of non-polluting sporangia or zoospores of Phytophthora nicotiana for monitoring the pathogenicity of Phytophthora nicotianae in various places, and determining the biological activity of various fungicides on their sporangia and zoospores. And determine the resistance level of loofah varieties. At present, there are mainly two methods for obtaining a large number of sporangia and zoospores of Phytophthora nicotiana indoors: ...

Claims

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Application Information

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IPC IPC(8): C12N3/00
Inventor 何自福蓝国兵罗方芳佘小漫
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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