Use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea

A technology of Botrytis cinerea and Streptomyces shioya, which is applied in the field of microorganisms and can solve problems such as insecurity

Inactive Publication Date: 2013-07-31
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

The technical effects described are two ways we use plants called Salinospora spp., with their ability to fight against certain types of bacteria or other harmful organisms like yeast spores (yeasts). This makes them ideal targets for developing pesticide products targeted specifically towards these specific crops without causing any negative side-effects such as reducing crop yields caused by insect damage.

Problems solved by technology

This patents describes how certain types of organisms called phytoliths (plant root parasitism) help protect crops against harmful pests like soil borne nematodes or mitochondria. However, these natural sources also contain beneficial compounds which could potentially benefit human health if used properly.

Method used

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  • Use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea
  • Use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea
  • Use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea

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Embodiment 1

[0018] Embodiment 1: (isolation and screening of Streptomyces shioya 107)

[0019] The present invention collected Chinese wolfberry stems in Hangzhou, Zhejiang in July 2012, and obtained Streptomyces shiyaya from them through the steps of surface disinfection, endophyte isolation, cultivation, fermentation, activity determination, and screening of active strains of pathogenic bacteria that inhibit plant fungal diseases. 107. Save.

[0020] Determination of antibacterial activity of Streptomyces shioya 107: Take 1mL of the fermentation supernatant filtered through a 0.22μm membrane in a sterile petri dish, mix it with 9mL of PDA medium cooled to 50°C, and place on each medium plane after cooling Put 1 rice sheath blight fungus (Thanatephorus cucumeris) or cucumber wilt fungus (Fusarium oxysporum.sp.cucumebrium Owen) or cucumber blight fungus (Rhizoctonia solani) or watermelon wilt fungus (F.oxysporum f.sp. niveum) or tomato cinerea (Botrytis cinerea) etc. for test bacterial c...

Embodiment 2

[0023] Embodiment 2: (the preparation method of Streptomyces shioya 107 fermentation metabolites)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the isolated and preserved Streptomyces shioya 107 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C±1°C until the inclined surface of the test tube is covered with spores for inoculation;

[0026] (2) Fermentation culture: the fermentation culture liquid contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, 5g of peptone in every 1000mL, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 10g, 60mL of fermentation broth per 300mL Erlenmeyer bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick a spore of Streptomyces platinumensis 107 under sterile conditions and inoculate at 28°C±1°C , the shaker speed is 180 rpm, and the fermentation culture is 128h;

[0027] (3) Extraction of fermented product: After the fermentation is completed, the fermented li...

Embodiment 3

[0029] Embodiment 3: (the inhibitory effect of Streptomyces shioya 107 metabolites to rice sheath blight, cucumber solanum blight, tomato Botrytis cinerea, cucumber fusarium wilt and watermelon wilt)

[0030] (1) Accurately weigh 0.20 g of the metabolite of Streptomyces shioya 107, add 10 mL of sterile water, dissolve it fully by ultrasonic vibration, and finally prepare a mother solution with a concentration of 20 g / L;

[0031] (2) Determination of antibacterial activity: Take the above-mentioned mother liquor and dilute it into solutions with a concentration of 2g / L and 1g / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C. Evenly (the final concentration of the metabolites of Streptomyces shioya 107 is 200mg / L and 100mg / L), after cooling, put a 4mm diameter of rice sheath blight or cucumber solanum blight or tomato ash on each medium plane Bacteria cakes of mold fungi or cucumber wilt bact...

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Abstract

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

Description

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Claims

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Application Information

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Owner CHINA JILIANG UNIV
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