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Use of metabolites of nigrospora oryzae 46 in prevention of colletotrichum lindemuthianum

A technology of black spore fungus and kidney bean anthrax, which is applied in the field of microorganisms and can solve the problems of unsafe microbial source pesticide types and other problems

Inactive Publication Date: 2011-10-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to aim at the deficiency that chemical pesticide is unsafe and microbial source pesticide kind is less, provides a kind of endophytic fungus-rice black spore fungus (Nigrospora oryzae) that isolates and screens out from oleander; Another aspect of the present invention The purpose is to provide a method for using the strain metabolites to prevent and treat plant fungal diseases

Method used

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  • Use of metabolites of nigrospora oryzae 46 in prevention of colletotrichum lindemuthianum
  • Use of metabolites of nigrospora oryzae 46 in prevention of colletotrichum lindemuthianum
  • Use of metabolites of nigrospora oryzae 46 in prevention of colletotrichum lindemuthianum

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Embodiment 1

[0018] Embodiment 1: (Isolation and Screening of N. oryzae 46)

[0019] The present invention collected oleander stems in Zhejiang Academy of Agricultural Sciences in June 2008, and obtained them through the steps of surface disinfection, separation of endophytic fungi, cultivation, fermentation, activity determination, and screening of effective strains for inhibiting plant fungal disease pathogens. N. oryzae 46, preserved.

[0020] Determination of the antibacterial activity of Nigeria oryzae 46: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. After cooling, place on each medium plane Put 1 piece of bacterium cake for testing such as cucumber blight fungus (Rhizoctonia solani) or bean anthracnose fungus (Colletotrichum lindemuthianum) or tomato gray mold fungus (Botrytis cinerea) with a diameter of 4 mm respectively, and the fungus cake is connected to the cent...

Embodiment 2

[0023] Embodiment 2: (preparation method of nigrosporium oryzae 46 fermentation metabolites)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the 46 strains of N. oryzae isolated and preserved to a solid plate of potato dextrose PDA, and cultivate them at 28°C±1°C until the hyphae cover the entire petri dish. A 4mm hole puncher is punched to obtain a fungus cake for inoculation;

[0026] (2) Fermentation culture: The fermentation culture medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 128 hours;

[0027] (3) Extraction of fermented product: After the fermentatio...

Embodiment 3

[0029] Embodiment 3: (inhibitory effect of Nigrosporum oryzae 46 metabolites on cucumber solanum blight, kidney bean anthracnose and tomato Botrytis cinerea)

[0030] (1) Accurately weigh 0.03g of the metabolite of Nigeria oryzae 46, add 10mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother solution with a concentration of 3g / L;

[0031] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1500mg / L and 1000mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C Evenly (the final concentration of the metabolites of Nigersporium oryzae 46 is 150mg / L and 100mg / L), after cooling, put a 4mm diameter of bean anthracnose or cucumber blight or tomato Botrytis cinerea respectively on each medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter...

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Abstract

The invention relates to the technical field of microbe. Dogbane endophytic fungus provided by the present invention is isolated from a living dogbane collected in Zhejiang Academy of Agricultural Sciences through an endophytic fungi separation technology. The dogbane endophytic fungus is identified through microbial taxonomy, and is named as nigrospora oryzae 46. The nigrospora oryzae 46 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The preservation date is 2010 / 12 / 31, and the preservation register number is CGMCC No.4509. The nigrospora oryzae 46 is characterized in that: the nigrospora oryzae 46 can generate active metabolites through a liquid fermentation, and the active metabolites provide inhibitions for rhizoctonia solani, colletotrichum lindemuthianum and botrytis cinerea. Therefore, the nigrospora oryzae 46 is an important microbe resource in agriculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of a biocontrol endophyte fungus Nigrospora oryzae and the application of its metabolites. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant endoph...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00C12P1/02C12R1/645
Inventor 俞晓平申屠旭萍董胜张郝培应边亚琳马正
Owner CHINA JILIANG UNIV
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