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Biocontrol endophytic fungi-Alternaria alternata

A technology of Alternaria and microbial strains, which is applied in the field of microorganisms and can solve problems such as the types of unsafe microbial source pesticides

Inactive Publication Date: 2011-09-21
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of endophytic fungus-Alternaria alternata (Alternaria alternata) that isolates and screens out from camphor tree for chemical pesticide unsafe and microbial source pesticide kind less; Another purpose of the present invention is Provides a method for preventing and treating plant fungal diseases by utilizing the metabolites of the strain

Method used

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  • Biocontrol endophytic fungi-Alternaria alternata
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  • Biocontrol endophytic fungi-Alternaria alternata

Examples

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Embodiment 1

[0018] Embodiment 1: (isolation and screening of Alternaria 31)

[0019] The present invention collected camphor tree stems in Tianmu Mountain, Hangzhou, Zhejiang in June 2008, and obtained Alternaria sp. 31. Save.

[0020] Determination of the antibacterial activity of Alternaria 31: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. Put 1 piece of bacterium cake for testing such as cucumber blight fungus (Rhizoctonia solani) or watermelon wilt fungus (Fusarium oxysporium) or tomato gray mold fungus (Botrytis cinerea) with a diameter of 4mm respectively, and the fungus cake is connected to the center of the petri dish (petri dish 9 cm in diameter), placed in an incubator for 72 hours at 28°C, treated with sterile water as a control, and repeated 3 times; the diameter of the colony was measured by the cross method, and the inhibition rate was calculated by the foll...

Embodiment 2

[0023] Embodiment 2: (the preparation method of Alternaria 31 fermentation metabolite)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the isolated and preserved Alternaria 31 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C ± 1°C until the hyphae cover the entire petri dish. A 4mm hole puncher is punched to obtain a fungus cake for inoculation;

[0026] (2) Fermentation culture: The fermentation culture medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH per 1000mL. 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 126 hours;

[0027] (3) Extraction of fermented product: After the fermentation...

Embodiment 3

[0029] Embodiment 3: (the inhibitory effect of Alternaria 31 metabolites to cucumber solanum blight, watermelon wilt and tomato Botrytis cinerea)

[0030] (1) Accurately weigh 0.18 g of the metabolite of Alternaria 31, add 10 mL of sterile water, fully dissolve with ultrasonic vibration, and finally prepare a mother solution with a concentration of 18 g / L;

[0031] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1800mg / L and 900mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C (Final concentrations of Alternaria 31 metabolites are 180mg / L and 90mg / L), and after cooling, place a 4mm-diameter watermelon wilt or cucumber blight or tomato Botrytis cinerea on each medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), placed in an incubator...

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Abstract

The invention provides biocontrol endophytic fungi- Alternaria alternata and relates to the technical field of microorganisms. The endophytic fungi of camphor trees are separated from camphor tree living bodies collected from Tianmu Mountain in Hangzhou, Zhejiang by an endophytic fungi separation technique and are named as Alternaria alternata 31 according to microbial taxonomy identification. The biocontrol endophytic fungi-Alternaria alternata is collected in the General Microbiology Center of China Microorganism Strain Collection Management Committee, wherein the collection date is 2010.12.31 and the collection registered number is CGMCC No.4508. The biocontrol endophytic fungi-Alternaria alternata has the most remarkable characteristic that: the strain liquid is fermented to generate active metabolites which can inhibit plant fungi disease pathogenic bacteria such as rhizoctonia solani, fusarium oxysporium, botrytis cinerea and the like and is important microbial resources agriculturally.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of the biocontrol endophytic fungus Alternaria alternata and the application of its metabolites. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01P3/00C12R1/645
Inventor 申屠旭萍俞晓平董胜张郝培应边亚琳马正
Owner CHINA JILIANG UNIV
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