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40 results about "Alternaria sp" patented technology
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Alternaria radicina bacterial strain for producing alpha-L-rhamnoside enzyme and cultivating method as well as application thereof
ActiveCN101914451AStrong hydrolytic activityHydrolysis Efficient CatalysisFungiMicroorganism based processesNaringinFruit juice
The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.
Owner:SHANDONG UNIV
Lilium regale glutathione S-transferase gene LrGSTU5 and application thereof
InactiveCN103194466AEnhanced fungal activityStrong in vitro antifungal activityTransferasesFermentationBiotechnologyFusarium oxysporum
The invention discloses a glutathione S-transferase gene LrGSTU5, wherein the nucleotide sequence of the gene is shown as SEQ ID NO: 1; and the gene encodes the glutathione S-transferase. According to the invention, related technology of functional genomics is adopted to prove that the lilium regale LrGSTU5 gene has the function of improving the antifungal ability of plants; the lilium regale glutathione S-transferase gene LrGSTU5 provided by the invention is built on plant expression carriers and then transferred into tobacco for overexpression; the transgenic tobacco has a high in-vitro antifungal activity; and the transgenic tobacco protein expressing LrGSTU5 has an obvious inhibition effect on the growth of various pathogenic fungi such as botryosphaeria dothidea, fusarium oxysporum, alternaria sp and the like.
Owner:KUNMING UNIV OF SCI & TECH
Euonymus japonicus endophytic bacterium bacillus methylotrophicus and application thereof
The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.
Owner:NANJING FORESTRY UNIV
Anthraquinone dimer derivative Alterporriol P and preparation method and application thereof
ActiveCN102633616ASignificant proliferation inhibitory activityOrganic chemistryMicroorganism based processesSilica gelFermentation
The invention relates to an anthraquinone dimer derivative and a preparation method and application thereof. The preparation method comprises the following steps of: performing strain culture on endophytic fungi Alternaria sp. (ZJ-2008003) from alcyonacea Sarcophyton sp. in South China Sea in a strain culture medium, performing fermentation cultivation in a fermentation medium, filtering the obtained fermentation solution to obtain thalli, and extracting the obtained thalli by using methanol; and concentrating the extract, performing silica-gel column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography preparation, concentrating the obtained eluate, and thus obtaining red powder, namely the anthraquinone dimer derivative Alterporriol P. The anthraquinone dimer derivative from the marine fungi has a strong effect of resisting cell proliferation of human prostatic cancer PC-3 and human colon cancer HCT-116, and can be used as a cell proliferation inhibitor or an anti-tumor agent; and the raw material can be produced in large scale, so the derivative has a broad application prospect.
Owner:OCEAN UNIV OF CHINA
Anthraquinone dimer derivative and preparation method and application thereof
The invention relates to anthraquinone dimer derivatives and a preparation method and application thereof. The preparation method comprises the steps of firstly conducting strain culture of an endophytic fungus Alternaria sp. (ZJ-2008003) coming from South China Sea soft coral Sarcophyton Sp. in a strain culture medium, conducting fermentation culture in a fermentation culture medium, filtering the obtained fermentation liquid, removing filtrate to obtain thalli, extracting the thalli by using methanol, concentrating the extract, conducting silica gel column chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatographic preparation, concentrating the eluent to obtain red powder, which is the anthraquinone dimer derivatives alterporriol Q and alterporriol R. The anthraquinone dimmer derivatives coming from marine fungi have a strong antiviral effect, can effectively inhibit porcine reproductive and respiratory syndrome viruses (PRRSV) and can be used as antiviral drugs, raw materials can be produced in a large scale and the application prospect is wide.
Owner:OCEAN UNIV OF CHINA
Fungus of alternaria sp., preparation method of secondary metabolite of fungus and application of secondary metabolite as microbial acaricide
The invention discloses an endophytic fungus of alternaria sp. separated from a medicago seed, wherein the endophytic fungus is collected with the number of CGMCCNo.9277. A secondary metabolite of the fungus has remarkable contact activity for agricultural harmful mites, but has no any influences to crops at a relatively low concentration. Therefore, the secondary metabolite serving as a safe, nontoxic and environment-friendly microbial acaricide has significance and favorable application prospect in preparation of a miticide.
Owner:LANZHOU UNIVERSITY
Alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content
A alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6380, and the name of the strain is 11775-42-Z1, and is 42# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 42# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 42# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Cunninghamia lanceolata endophytic fungus and biological prevention and treatment application thereof
The invention relates to a cunninghamia lanceolata endophytic fungus and a biological prevention and treatment application thereof. The cunninghamia lanceolata endophytic fungus is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M 2019895, and the preservation date is November 4, 2019. The Epicoccum dendrobii SMEL of the invention is the cunninghamialanceolata endophytic fungus, and can inhibit the growth of Cunninghamia lanceolata colletotrichum and reduce the biomass accumulation amount of Cunninghamia lanceolata colletotrichum; the inoculation test result shows that the plaque development of cunninghamia lanceolata anthracnose is significantly inhibited after inoculation with SMEL bacteria; and the SMEL strain has certain antagonistic effects against Cunninghamia lanceolata colletotrichum, Colletotrichum gossypii, Rhizoctonia sp., Cytospora chrysosperm, Fusicoccum aesculi and Alternaria sp. on the plate.
Owner:NANJING FORESTRY UNIV
Anthraquinone derivative, preparation method and application thereof
ActiveCN102531868AStrong antiviral activityOrganic active ingredientsOrganic chemistryEthyl acetateFermentation
The invention relates to an anthraquinone derivative, a preparation method and application thereof. During preparation, entomopathogenic fungi in soft coral Alternaria sp. (ZJ-2008003) is first placed in a strain culturing medium for strain culturing and then placed in a fermentation culturing medium for fermentation culturing, obtained fermentation liquor is filtered, thalli are removed to obtain filtrate, and the filtrate is extracted through ethyl acetate; after extract liquor is concentrated, silicagel column chromatography, sephadex LH-20 gel column chromatography and half-preparation of high performance liquid chromatography preparation are conducted, obtained eluent is concentrated, and clear crystals are obtained, namely, the compound of tetrahydroaltersolanols C-E; pink powder is obtained, namely, the compound of tetrahydroaltersolanol F. The anthraquinone derivative originating from marine fungi has strong antiviral effect, can effectively restrain porcine reproductive and respiratory syndrome virus (PRRSV), can be used as antiviral drug, and has broad application prospects due to the fact that raw materials can be produced in a large scale.
Owner:OCEAN UNIV OF CHINA
Sesquiterpene hybrid hydroquinone compounds and preparation method and application thereof
The invention relates to a preparation method and application of two sesquiterpene hybrid hydroquinone compounds. The sesquiterpene hybrid hydroquinone compounds with novel structures are produced by adopting alternaria sp. SP-32 in a South China Sea sponge sample. Proved by experiments, the compounds have flu virus A H1N1 resistant effect, and have potential for developing flu virus A resistant medicament preparations.
Owner:OCEAN UNIV OF CHINA
Endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and application thereof to biocontrol
The invention discloses endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and an application thereof to biocontrol. Sampling from Fenghuang Dancong tea plant leaves, separation and purification, screening and cultivation are carried out in order to obtain a strain of Serratia sp. ZF05 GDMCC No:60015 with good biocontrol effects. The bacterial strain is obviously different from common Serratia sp. in the aspects of physiological-biochemical characteristics and molecular level, so the bacterial strain is a typical new bacterial strain. The bacterial strain ZF05 has good inhibition effects for Alternaria alternate, Walnut blight, Alternaria sp., Rhizoctonia solani, and Pythium aphanidermatum; mycelium growth inhibition rates are as follows: 73.02%, 73.70%, 78.51%, 84.56% and 20.06%; Serratia sp. ZF05 is a biocontrol bacterium with obvious typicality and high specificity, and has good application prospects for developing new biopesticides or biological control microbial inoculum; Serratia sp. ZF05 has wide application values for biocontrol.
Owner:HANSHAN NORMAL UNIV
Sea-buckthorn endophytic fungi, preparation method of its extract product and application thereof
ActiveCN104911108AImprove drought resistanceHigh activityPlant growth regulatorsBiocideBiotechnologyEndophyte
The invention relates to a strain of fungi in wild sea-buckthorn root from Changbai Mountain and an application of the fungi for increasing turfgrass drought tolerance. The sea-buckthorn endophytic fungus is Alternaria Sp, a preservation number is CGMCC No. 10117, the preservation date is 11th, Dec, 2014, and the endophytic fungus is preserved by China General Microbiological Culture Collection Center. The concentration of a mycelia extract product is nanogram level, so that the endophytic fungi extract product has high activity, and the endophytic fungus can induce the turfgrass to obtain good drought tolerance by spraying the turfgrass with 0.5ng / ml concentration.
Owner:LIAONING ACAD OF AGRI SCI
Alternaria sp strain GW61 and application thereof
The invention discloses an Alternaria sp strain GW61 CGMCC No. 21070 and application thereof in preparing Alternaria phenol. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC for short, address: #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing) as a patent strain on November 27, 2020, and the registration number of the strain in the preservation center is CGMCC No. 21070. The strain effectively solves the technical problem in the industry of preparing high-purity Alternaria phenol standard products.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
Alternaria radicina bacterial strain for producing alpha-L-rhamnoside enzyme and cultivating method as well as application thereof
ActiveCN101914451BStrong hydrolytic activityHydrolysis Efficient CatalysisFungiMicroorganism based processesNaringinFruit juice
The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.
Owner:SHANDONG UNIV
Endophytic fungus capable of promoting phosphorus absorption of aleurites montana
The invention relates to endophytic fungus capable of promoting phosphorus absorption of aleurites montana. The endophytic fungus is alternaria sp. ZY4 and has been registered and preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 30th, 2015. The preservation number is CGMCC No. 10328. According to the invention, the endophytic fungus ZY4 of aleurites montana is inoculated to land culture seedling of aleurites montana by a bacteria liquid spraying method, and content of phosphor in aleurites montana is measured after 60 days of inoculation. The difference of phosphorus content between aleurites montana treated by the inoculated strain ZY4 and a contrast is significant. The strain ZY4 treatment is more beneficial to promote phosphorus absorption of a plant than a contrast.
Owner:FUJIAN AGRI & FORESTRY UNIV
Anthraquinone derivatives and preparation method and application thereof
ActiveCN102531868BStrong antiviral activityOrganic active ingredientsOrganic chemistryEthyl acetateFermentation
The invention relates to an anthraquinone derivative, a preparation method and application thereof. During preparation, entomopathogenic fungi in soft coral Alternaria sp. (ZJ-2008003) is first placed in a strain culturing medium for strain culturing and then placed in a fermentation culturing medium for fermentation culturing, obtained fermentation liquor is filtered, thalli are removed to obtain filtrate, and the filtrate is extracted through ethyl acetate; after extract liquor is concentrated, silicagel column chromatography, sephadex LH-20 gel column chromatography and half-preparation of high performance liquid chromatography preparation are conducted, obtained eluent is concentrated, and clear crystals are obtained, namely, the compound of tetrahydroaltersolanols C-E; pink powder is obtained, namely, the compound of tetrahydroaltersolanol F. The anthraquinone derivative originating from marine fungi has strong antiviral effect, can effectively restrain porcine reproductive and respiratory syndrome virus (PRRSV), can be used as antiviral drug, and has broad application prospects due to the fact that raw materials can be produced in a large scale.
Owner:OCEAN UNIV OF CHINA
A kind of anthraquinone dimer derivative and its preparation method and application
The invention relates to anthraquinone dimer derivatives and a preparation method and application thereof. The preparation method comprises the steps of firstly conducting strain culture of an endophytic fungus Alternaria sp. (ZJ-2008003) coming from South China Sea soft coral Sarcophyton Sp. in a strain culture medium, conducting fermentation culture in a fermentation culture medium, filtering the obtained fermentation liquid, removing filtrate to obtain thalli, extracting the thalli by using methanol, concentrating the extract, conducting silica gel column chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatographic preparation, concentrating the eluent to obtain red powder, which is the anthraquinone dimer derivatives alterporriol Q and alterporriol R. The anthraquinone dimmer derivatives coming from marine fungi have a strong antiviral effect, can effectively inhibit porcine reproductive and respiratory syndrome viruses (PRRSV) and can be used as antiviral drugs, raw materials can be produced in a large scale and the application prospect is wide.
Owner:OCEAN UNIV OF CHINA
Marine fungi fermentation extract and application thereof as anti-senile dementia drug
ActiveCN108048488AImprove biological activityStrong inhibitory activityNervous disorderMicroorganismsAcetic acidRotary evaporator
The invention relates to a marine fungi fermentation extract and application thereof as an anti-senile dementia drug. The extract is prepared according to the following steps: (1) under aseptic conditions, inoculating marine fungi Alternaria sp. W-1 into a PD liquid culture medium, and carrying out constant temperature shaking table shaking culture to obtain a seed solution, wherein the temperature is 25 to 30 DEG C, the shaking table rotating speed is 150 to 180 rpm, and the culture time is 2 to 4 d; (2) inoculating the obtained seed solution obtained in the step (1) into a czapek's medium toobtain a fermentation matter, wherein the pH of the medium is 4.0 to 8.0, the inoculum amount is 5% to 20%, the culture temperature is 22 to 34 DEG C, and the culture time is 6 to 14 d; and (3) filtering the fermentation matter obtained in the step (2) with three to five layers of sterile gauze to obtain a filter liquor, extracting for two to four times with ethyl acetate after concentration through a rotary evaporator, merging an organic phase, and thus obtaining the extract after concentration through the rotary evaporator.
Owner:QINGDAO AGRI UNIV
Alternarlactam and preparation method and application thereof
InactiveCN101921711BOvercoming Drug Resistance DeficienciesSimple processOrganic active ingredientsFungiChemical synthesisCancer cell
Owner:NANJING UNIV
An Alternaria fungus that increases the yield and total phenolic acid content of Salvia miltiorrhiza
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
A kelp endophytic fungus and its application in the preparation of biologically active extracts
InactiveCN108048337BImprove biological activityStrong inhibitory activityFungiNervous disorderMicroorganismAlternaria sp
The present invention relates to a kelp endophytic fungus and its application in the preparation of biologically active extracts. The strain preservation information of the kelp endophytic fungus is: name of depository unit: General Microbiology Center of China Microbial Strain Preservation Management Committee; preservation Unit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; date of deposit: January 2, 2018; deposit number: CGMCC No.15181; classification name: Alternaria sp.
Owner:QINGDAO AGRI UNIV
Bacterial strain capable of degrading diuron and application of bacterial strain
ActiveCN114317290ASolve residual problemsImprove quality and safetyFungiWater contaminantsBiotechnologyAlternaria sp
The invention discloses a strain capable of degrading diuron and application of the strain. The bacterial strain is an Alternaria sp. Bacterial strain QHSH-5, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No. 23258. The strain can be used for degrading pesticide diuron, is beneficial to solving the problem of agricultural diuron residues, and improves the quality safety of agricultural products.
Owner:NINGXIA MEDICAL UNIV +1
Monoclonal Antibody Recognizing Alternaria and Its Hybridoma Cell Line aaa1
ActiveCN110862452BStrong specificityHigh detection sensitivityTissue cultureImmunoglobulins against fungi/algae/lichensIntraperitoneal routePlant disease
The invention discloses a monoclonal antibody that recognizes Alternaria and a hybridoma cell line thereof. The monoclonal antibody that recognizes Alternaria is secreted by a hybridoma cell strain with a deposit number of CCTCC No: C2019218 ; The hybridoma cell line is named AaA1, and was deposited in the China Center for Type Culture Collection (CCTCC) on October 17, 2019, and the preservation number is: CCTCC No: C2019218. This monoclonal antibody is used for the identification and dynamic monitoring of animal and plant diseases caused by Alternaria infection and the biological research of Alternaria. It can be obtained by intraperitoneal injection of this cell line into BaL b / c mice Compared with polyclonal antibodies, a large number of monoclonal antibodies have the advantages of high purity, strong specificity, and good repeatability.
Owner:ZHEJIANG CHINESE MEDICAL UNIVERSITY
Sesquiterpene hybrid hydroquinone compounds and preparation method and application thereof
Owner:OCEAN UNIV OF CHINA
Alternarlactam and preparation method and application thereof
InactiveCN101921711AEnhanced inhibitory effectOvercoming Drug Resistance DeficienciesOrganic active ingredientsFungiAbnormal tissue growthChemical synthesis
The invention belongs to the technical field of microbial engineering and pharmaceutical engineering and particularly relates to alternarlactam extracted from a solid fermentation material of maubertiana boott endophytic fungi (Alternaria sp.) and a preparation method and anti-tumor effect thereof. The alternarlactam can be prepared by a solid fermentation method and a chemical synthesis method. The alternarlactam has the effects of strongly inhibiting the proliferation of human cervical cancer cells HeLa and human liver cancer cells QSG-7701, so that the alternarlactam can be used as a compound having the anti-tumor effect and is applied to preparing anti-tumor medicaments.
Owner:NANJING UNIV
The endophytic fungus strain hubu0122 of Dirt grass and its application
ActiveCN113416656BHas broad-spectrum antimicrobial activityAntibacterialBiocideFungiBiotechnologyHelicoverpa
The present invention relates to the endophytic fungus strain HUBU0122 of Dirtwort and its application. The endophytic fungus named Alternaria ( Alternaria sp. ) HUBU0122, whose strain preservation unit is the China Type Culture Collection Center, the preservation address is No. 299 Bayi Road, Wuhan City, Hubei Province, the preservation date is April 19, 2021, and the preservation number is CCTCC No.M2021414. The bacterium has antibacterial effect on Salmonella and Xanthomonas oryzae, and can be used as a utilization resource of plant endophytic fungi, laying the foundation for obtaining new agricultural antibiotic natural ingredients that resist plant pathogenic fungal diseases.
Owner:HUBEI UNIV
Anthraquinone dimer derivative alterporriol P and its preparation method and application
ActiveCN102633616BStrong antiproliferative activityOrganic chemistryMicroorganism based processesSilica gelFermentation
Owner:OCEAN UNIV OF CHINA
Bacillus new species with Colletotrichum fragariae inhibition activity, and application of bacillus new species
ActiveCN113151092AReduce weight lossEasy to keepFruit and vegetables preservationBacteriaBiotechnologyColletotrichum fragariae
The invention provides a bacillus which is a new species of the bacillus, is called after Bacillus sp. Nov. QN1NO-4 and is collected in the China Center for Type Culture Collection on March 30, 2021 with the collection number of CCTCC M 2021306. The bacillus has stable broad-spectrum antagonistic activity and has a powerful anti-bacteria function for common crop pathogenic fungi, including Fusarium graminearum, Curvularia lunata, Colletotrichum gloeosporioides, Fusarium oxysporum f. sp. cucumebrium, Colletotrichum fragariae, Colletotrichum gloeosporioides penz., Pyricularia oryzae, Colletotrichum capsici, Alternaria sp., Colletotrichum acutatum and the like, the fermentation extract of the bacillus can effectively prevent pathogenic hyphae from infecting isolated fruits and the like, the weight loss ratio of the fruit is lowered, soluble solid matters can be favorably kept, the safe source of the effective ingredient of a biocontrol preparation is increased, a new field is widened for preventing and treating various plant diseases, including the Colletotrichum fragariae and the like, and the bacillus has a good development and application prospect.
Owner:INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
A marine fungus fermented extract and its application as an anti-senile dementia drug
ActiveCN108048488BImprove biological activityStrong inhibitory activityNervous disorderMicroorganismsLiquid mediumRotary evaporator
The present invention relates to a marine fungus fermented extract and its application as an anti-senile dementia drug. The extract is prepared by the following steps: (1) under sterile conditions, the marine fungus Alternaria sp.W‑1 is inoculated into PD In the liquid medium, vibrate culture on a constant temperature shaker, the temperature is 25-30°C, the speed of the shaker is 150-180r / min, and the culture time is 2-4d to obtain a seed solution; (2) the seeds obtained in step (1) The solution is inoculated into the Cha's medium, the pH of the medium is 4.0-8.0, the inoculum size is 5-20%, the culture temperature is 22-34°C, and the culture time is 6-14d to obtain a fermented product; (3) the step ( 2) The fermented product obtained is filtered with 3-5 layers of sterile gauze to obtain the filtrate, which is concentrated by a rotary evaporator, extracted 2-4 times with ethyl acetate, combined with the organic phases, concentrated by a rotary evaporator to obtain the extracted thing.
Owner:QINGDAO AGRI UNIV
M-4 strain for degradation of waste castor-based lubricating oil and mineral oil
The invention relates to an M-4 strain for degradation of waste castor-based lubricating oil and mineral oil. The M-4 strain is characterized in that a category name of the M-4 strain is Alternaria sp.; a preservation number is CGMCC No. 4933; a preservation date is June 10, 2011; the preservation organization name is China general microbiological culture collection center; and the preservation address is No. 3, Court 1, Beichen West Road, Chaoyang District, Beijing City. Through a plate gradient coating separation method, a soil sample and a water sample are collected from a place around a sewage outlet of a castor oil squeezing workshop and a microorganism which grows through depending on castor-based lubricating oil as an only carbon source is screened out. The plate gradient coating separation method has simple, convenient and fast operation processes. The M-4 strain for degradation of castor-based lubricating oil can grow fast, is suitable for expansion re-culture under the conditions of an optimum culture temperature and a pH value and can be prepared into a strain starter to be dropped into a polluted environment for ecological restoration.
Owner:TIANJIN UNIV OF SCI & TECH
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