46 results about "Alternaria sp" patented technology

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

The invention discloses a glutathione S-transferase gene LrGSTU5, wherein the nucleotide sequence of the gene is shown as SEQ ID NO: 1; and the gene encodes the glutathione S-transferase. According to the invention, related technology of functional genomics is adopted to prove that the lilium regale LrGSTU5 gene has the function of improving the antifungal ability of plants; the lilium regale glutathione S-transferase gene LrGSTU5 provided by the invention is built on plant expression carriers and then transferred into tobacco for overexpression; the transgenic tobacco has a high in-vitro antifungal activity; and the transgenic tobacco protein expressing LrGSTU5 has an obvious inhibition effect on the growth of various pathogenic fungi such as botryosphaeria dothidea, fusarium oxysporum, alternaria sp and the like.

The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.

The invention relates to an anthraquinone dimer derivative and a preparation method and application thereof. The preparation method comprises the following steps of: performing strain culture on endophytic fungi Alternaria sp. (ZJ-2008003) from alcyonacea Sarcophyton sp. in South China Sea in a strain culture medium, performing fermentation cultivation in a fermentation medium, filtering the obtained fermentation solution to obtain thalli, and extracting the obtained thalli by using methanol; and concentrating the extract, performing silica-gel column chromatography, Sephadex LH-20 gel column chromatography and high performance liquid chromatography preparation, concentrating the obtained eluate, and thus obtaining red powder, namely the anthraquinone dimer derivative Alterporriol P. The anthraquinone dimer derivative from the marine fungi has a strong effect of resisting cell proliferation of human prostatic cancer PC-3 and human colon cancer HCT-116, and can be used as a cell proliferation inhibitor or an anti-tumor agent; and the raw material can be produced in large scale, so the derivative has a broad application prospect.

The invention relates to anthraquinone dimer derivatives and a preparation method and application thereof. The preparation method comprises the steps of firstly conducting strain culture of an endophytic fungus Alternaria sp. (ZJ-2008003) coming from South China Sea soft coral Sarcophyton Sp. in a strain culture medium, conducting fermentation culture in a fermentation culture medium, filtering the obtained fermentation liquid, removing filtrate to obtain thalli, extracting the thalli by using methanol, concentrating the extract, conducting silica gel column chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatographic preparation, concentrating the eluent to obtain red powder, which is the anthraquinone dimer derivatives alterporriol Q and alterporriol R. The anthraquinone dimmer derivatives coming from marine fungi have a strong antiviral effect, can effectively inhibit porcine reproductive and respiratory syndrome viruses (PRRSV) and can be used as antiviral drugs, raw materials can be produced in a large scale and the application prospect is wide.

The invention discloses an endophytic fungus of alternaria sp. separated from a medicago seed, wherein the endophytic fungus is collected with the number of CGMCCNo.9277. A secondary metabolite of the fungus has remarkable contact activity for agricultural harmful mites, but has no any influences to crops at a relatively low concentration. Therefore, the secondary metabolite serving as a safe, nontoxic and environment-friendly microbial acaricide has significance and favorable application prospect in preparation of a miticide.

A alternaria sp. fungi improving salvia miltiorrhiza output and total phenolic-acid content belongs to cladosporium sp. and has the preservation number of CGMCC No. 6380, and the name of the strain is 11775-42-Z1, and is 42# for short. When salvia miltiorrhiza is cultivated, 0.5-1.5 g of 42# fungal material is applied to the root of each salvia miltiorrhiza seedling, the fungal material and the salvia miltiorrhiza seedling are subjected to symbiosis cultivation for 4-6 months, and the 42# fungal material helps to improve the output of salvia miltiorrhiza at harvest time and the content of salvia miltiorrhiza total phenolic acids and salvianolic acid B. The technological method is simple and practicable and free of pollution, is applicable to artificial cultivation of salvia miltiorrhiza, is capable of effectively improving the output and the quality of the medicinal material, and has good application prospect.

The invention relates to a cunninghamia lanceolata endophytic fungus and a biological prevention and treatment application thereof. The cunninghamia lanceolata endophytic fungus is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M 2019895, and the preservation date is November 4, 2019. The Epicoccum dendrobii SMEL of the invention is the cunninghamialanceolata endophytic fungus, and can inhibit the growth of Cunninghamia lanceolata colletotrichum and reduce the biomass accumulation amount of Cunninghamia lanceolata colletotrichum; the inoculation test result shows that the plaque development of cunninghamia lanceolata anthracnose is significantly inhibited after inoculation with SMEL bacteria; and the SMEL strain has certain antagonistic effects against Cunninghamia lanceolata colletotrichum, Colletotrichum gossypii, Rhizoctonia sp., Cytospora chrysosperm, Fusicoccum aesculi and Alternaria sp. on the plate.

The invention relates to an anthraquinone derivative, a preparation method and application thereof. During preparation, entomopathogenic fungi in soft coral Alternaria sp. (ZJ-2008003) is first placed in a strain culturing medium for strain culturing and then placed in a fermentation culturing medium for fermentation culturing, obtained fermentation liquor is filtered, thalli are removed to obtain filtrate, and the filtrate is extracted through ethyl acetate; after extract liquor is concentrated, silicagel column chromatography, sephadex LH-20 gel column chromatography and half-preparation of high performance liquid chromatography preparation are conducted, obtained eluent is concentrated, and clear crystals are obtained, namely, the compound of tetrahydroaltersolanols C-E; pink powder is obtained, namely, the compound of tetrahydroaltersolanol F. The anthraquinone derivative originating from marine fungi has strong antiviral effect, can effectively restrain porcine reproductive and respiratory syndrome virus (PRRSV), can be used as antiviral drug, and has broad application prospects due to the fact that raw materials can be produced in a large scale.

The invention relates to a preparation method and application of two sesquiterpene hybrid hydroquinone compounds. The sesquiterpene hybrid hydroquinone compounds with novel structures are produced by adopting alternaria sp. SP-32 in a South China Sea sponge sample. Proved by experiments, the compounds have flu virus A H1N1 resistant effect, and have potential for developing flu virus A resistant medicament preparations.

The invention discloses endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and an application thereof to biocontrol. Sampling from Fenghuang Dancong tea plant leaves, separation and purification, screening and cultivation are carried out in order to obtain a strain of Serratia sp. ZF05 GDMCC No:60015 with good biocontrol effects. The bacterial strain is obviously different from common Serratia sp. in the aspects of physiological-biochemical characteristics and molecular level, so the bacterial strain is a typical new bacterial strain. The bacterial strain ZF05 has good inhibition effects for Alternaria alternate, Walnut blight, Alternaria sp., Rhizoctonia solani, and Pythium aphanidermatum; mycelium growth inhibition rates are as follows: 73.02%, 73.70%, 78.51%, 84.56% and 20.06%; Serratia sp. ZF05 is a biocontrol bacterium with obvious typicality and high specificity, and has good application prospects for developing new biopesticides or biological control microbial inoculum; Serratia sp. ZF05 has wide application values for biocontrol.

The invention relates to a strain of fungi in wild sea-buckthorn root from Changbai Mountain and an application of the fungi for increasing turfgrass drought tolerance. The sea-buckthorn endophytic fungus is Alternaria Sp, a preservation number is CGMCC No. 10117, the preservation date is 11th, Dec, 2014, and the endophytic fungus is preserved by China General Microbiological Culture Collection Center. The concentration of a mycelia extract product is nanogram level, so that the endophytic fungi extract product has high activity, and the endophytic fungus can induce the turfgrass to obtain good drought tolerance by spraying the turfgrass with 0.5ng / ml concentration.

The invention discloses an Alternaria sp strain GW61 CGMCC No. 21070 and application thereof in preparing Alternaria phenol. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC for short, address: #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing) as a patent strain on November 27, 2020, and the registration number of the strain in the preservation center is CGMCC No. 21070. The strain effectively solves the technical problem in the industry of preparing high-purity Alternaria phenol standard products.

The invention relates to an alternaria radicina bacterial strain and a cultivating method as well as application thereof, in particular to an alternaria radicina bacterial strain capable of producing alpha-L-rhamnoside enzyme and a preparation method as well as the application to naringin hydrolysis. The invention provides an alternaria radicina L1 (Alternaria sp. Li) bacterial strain for producing alpha-L-rhamnoside enzyme, characteristics, a cultivating method and application to naringin hydrolysis. The bacterial strain is preserved in the China General Microbiological Culture Collection Center in March 25th, 2010; and the preservation number is CGMCC No.3688. The alternaria radicina L1 (Alternaria sp. Li) bacterial strain is simple in cultivation, has stable hereditary property and enzyme activity, high hydrolysis efficiency, strong industrialized production advantages and wide application potential, and can be used in the fields of enzymatic debittering, and the like of citrus juice in food industry.

The invention belongs to the technical field of deep sea microbial materials, and discloses a perylenequinone compound of Alternaria sp. Xiao20, a derivative of the perylenequinone compound, or a pharmaceutically acceptable salt of the perylenequinone compound, a preparation method of the perylenequinone compound, and an application of the perylenequinone compound and the derivative or the pharmaceutically acceptable salt of the perylenequinone compound in preparation of medicines for preventing and / or treating neuroinflammation diseases. The invention provides a perylenequinone compound or a derivative thereof separated from a fermentation product of alternaria alternata, and the perylenequinone compound or the derivative thereof has important significance on development of marine drugs; the perylenequinone compound or the derivative or the pharmaceutically acceptable salt of the perylenequinone compound disclosed by the invention can be used for inhibiting the release of a neuromicroglia inflammatory factor NO; according to the present invention, the release level of the LPS-induced nerve microglial cell inflammatory factor NO is significantly reduced, the technical effect of neuroinflammation disease treatment is achieved, and the LPS-induced nerve microglial cell inflammatory factor NO can be prepared, such that the LPS-induced nerve microglial cell inflammatory factor NO can be used for preparing the neuroinflammation disease prevention and / or treatment drug, and has important significance on neuroinflammation disease treatment and new drug development.

The invention relates to endophytic fungus capable of promoting phosphorus absorption of aleurites montana. The endophytic fungus is alternaria sp. ZY4 and has been registered and preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 30th, 2015. The preservation number is CGMCC No. 10328. According to the invention, the endophytic fungus ZY4 of aleurites montana is inoculated to land culture seedling of aleurites montana by a bacteria liquid spraying method, and content of phosphor in aleurites montana is measured after 60 days of inoculation. The difference of phosphorus content between aleurites montana treated by the inoculated strain ZY4 and a contrast is significant. The strain ZY4 treatment is more beneficial to promote phosphorus absorption of a plant than a contrast.

The present invention belongs to the field of microbial fermentation technology, and discloses a Platycladus orientalis extract, and its preparation method and application. The Platycladus orientalis extract is obtained by fermenting an Alternaria sp. C-1 strain on the basis of conventionally extracting quercetin from Platycladus orientalis leaves, and the quercetin yield in the obtained Platycladus orientalis extract is increased by 25%. The Alternaria sp. C-1 strain has a strain preservation number of GDMCC No: 65881. The fermentation method of Alternaria of the present invention is to inoculate the seed liquid of the Alternaria sp. C-1 into a liquid culture medium for fermentation and culture. The strain has a relatively short growth cycle and a fast reproduction rate, and can continuously produce quercetin. The quercetin content in the strain fermentation product can reach up to 75 mg / L. The obtained Platycladus orientalis extract has the beneficial technical effects of high quercetin content, stable quercetin yield, reduced consumption of Platycladus orientalis leaves, and environmental friendliness.

The invention relates to an anthraquinone derivative, a preparation method and application thereof. During preparation, entomopathogenic fungi in soft coral Alternaria sp. (ZJ-2008003) is first placed in a strain culturing medium for strain culturing and then placed in a fermentation culturing medium for fermentation culturing, obtained fermentation liquor is filtered, thalli are removed to obtain filtrate, and the filtrate is extracted through ethyl acetate; after extract liquor is concentrated, silicagel column chromatography, sephadex LH-20 gel column chromatography and half-preparation of high performance liquid chromatography preparation are conducted, obtained eluent is concentrated, and clear crystals are obtained, namely, the compound of tetrahydroaltersolanols C-E; pink powder is obtained, namely, the compound of tetrahydroaltersolanol F. The anthraquinone derivative originating from marine fungi has strong antiviral effect, can effectively restrain porcine reproductive and respiratory syndrome virus (PRRSV), can be used as antiviral drug, and has broad application prospects due to the fact that raw materials can be produced in a large scale.

The invention relates to anthraquinone dimer derivatives and a preparation method and application thereof. The preparation method comprises the steps of firstly conducting strain culture of an endophytic fungus Alternaria sp. (ZJ-2008003) coming from South China Sea soft coral Sarcophyton Sp. in a strain culture medium, conducting fermentation culture in a fermentation culture medium, filtering the obtained fermentation liquid, removing filtrate to obtain thalli, extracting the thalli by using methanol, concentrating the extract, conducting silica gel column chromatography, Sephadex LH-20 gel column chromatography and high-performance liquid chromatographic preparation, concentrating the eluent to obtain red powder, which is the anthraquinone dimer derivatives alterporriol Q and alterporriol R. The anthraquinone dimmer derivatives coming from marine fungi have a strong antiviral effect, can effectively inhibit porcine reproductive and respiratory syndrome viruses (PRRSV) and can be used as antiviral drugs, raw materials can be produced in a large scale and the application prospect is wide.

The invention relates to a marine fungi fermentation extract and application thereof as an anti-senile dementia drug. The extract is prepared according to the following steps: (1) under aseptic conditions, inoculating marine fungi Alternaria sp. W-1 into a PD liquid culture medium, and carrying out constant temperature shaking table shaking culture to obtain a seed solution, wherein the temperature is 25 to 30 DEG C, the shaking table rotating speed is 150 to 180 rpm, and the culture time is 2 to 4 d; (2) inoculating the obtained seed solution obtained in the step (1) into a czapek's medium toobtain a fermentation matter, wherein the pH of the medium is 4.0 to 8.0, the inoculum amount is 5% to 20%, the culture temperature is 22 to 34 DEG C, and the culture time is 6 to 14 d; and (3) filtering the fermentation matter obtained in the step (2) with three to five layers of sterile gauze to obtain a filter liquor, extracting for two to four times with ethyl acetate after concentration through a rotary evaporator, merging an organic phase, and thus obtaining the extract after concentration through the rotary evaporator.

The invention belongs to the technical field of microbial engineering and pharmaceutical engineering and particularly relates to alternarlactam extracted from a solid fermentation material of maubertiana boott endophytic fungi (Alternaria sp.) and a preparation method and anti-tumor effect thereof. The alternarlactam can be prepared by a solid fermentation method and a chemical synthesis method. The alternarlactam has the effects of strongly inhibiting the proliferation of human cervical cancer cells HeLa and human liver cancer cells QSG-7701, so that the alternarlactam can be used as a compound having the anti-tumor effect and is applied to preparing anti-tumor medicaments.

The present invention relates to a kelp endophytic fungus and its application in the preparation of biologically active extracts. The strain preservation information of the kelp endophytic fungus is: name of depository unit: General Microbiology Center of China Microbial Strain Preservation Management Committee; preservation Unit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences; date of deposit: January 2, 2018; deposit number: CGMCC No.15181; classification name: Alternaria sp.

The invention discloses a strain capable of degrading diuron and application of the strain. The bacterial strain is an Alternaria sp. Bacterial strain QHSH-5, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No. 23258. The strain can be used for degrading pesticide diuron, is beneficial to solving the problem of agricultural diuron residues, and improves the quality safety of agricultural products.

The invention provides a screening method of endophytic fungi for improving drought resistance of quinoa and application of the endophytic fungi. The endophytic fungi are obtained through separation and purification from stems and leaves of oats, fusarium sp O-2, O-51 and O-63 and alternaria sp O-66 are screened, and the endophytic fungi Fusarium sp O-2 and Alternaria sp O-66 which are high in drought resistance and growth promotion capacity are obtained. The endophytic fungi Fusarium sp O-2 and Alternania sp O-66 are inoculated around the root system of the quinoa, the physiological activity, the stress resistance and the yield of the quinoa can be remarkably improved under drought stress, and an efficient and feasible way is provided for solving the problem of quinoa planting in arid regions.

The invention discloses a monoclonal antibody that recognizes Alternaria and a hybridoma cell line thereof. The monoclonal antibody that recognizes Alternaria is secreted by a hybridoma cell strain with a deposit number of CCTCC No: C2019218 ; The hybridoma cell line is named AaA1, and was deposited in the China Center for Type Culture Collection (CCTCC) on October 17, 2019, and the preservation number is: CCTCC No: C2019218. This monoclonal antibody is used for the identification and dynamic monitoring of animal and plant diseases caused by Alternaria infection and the biological research of Alternaria. It can be obtained by intraperitoneal injection of this cell line into BaL b / c mice Compared with polyclonal antibodies, a large number of monoclonal antibodies have the advantages of high purity, strong specificity, and good repeatability.

The invention relates to a preparation method and application of two sesquiterpene hybrid hydroquinone compounds. The sesquiterpene hybrid hydroquinone compounds with novel structures are produced by adopting alternaria sp. SP-32 in a South China Sea sponge sample. Proved by experiments, the compounds have flu virus A H1N1 resistant effect, and have potential for developing flu virus A resistant medicament preparations.

The invention belongs to the technical field of microbial fermentation, and discloses a platycladus orientalis extract as well as a preparation method and application thereof. The platycladus orientalis extract is prepared by fermenting an Alternaria sp. C-1 strain on the basis of conventionally extracting quercitrin from platycladus orientalis leaves, and the yield of quercitrin in the finally obtained platycladus orientalis extract is increased by 25%. The preservation number of the strain of the Alternaria sp. (Alternaria sp.) C-1 is GDMCC No: 65881, and the preservation number of the strain is GDMCC No: 65881. According to the method for fermenting the Alternaria sp., a liquid culture medium is inoculated with a seed solution of the Alternaria sp. C-1 for fermentation culture, the strain has a relatively short growth cycle and a high propagation speed, quercitrin can be continuously produced, the highest quercitrin content in a strain fermentation product can reach 75 mg / L, and the quercitrin content in the strain fermentation product can reach 75 mg / L; the obtained platycladus orientalis extract has the beneficial technical effects of high quercitrin content, stable quercitrin yield, reduction of consumption of the platycladus orientalis leaves, environmental friendliness and the like.

The present invention belongs to the technical field of microbial agents and discloses a strain of Acremonium crossbranchii NtSi1 and its use in preparing a root rot prevention and control agent. The Acremonium crossbranchii NtSi1 bacterial solution provided by the present invention has a wide range of inhibitory effects on plant pathogens and can prevent and control the root rot caused by Fusarium oxysporum ( Fusarium oxysporum )、Fusarium solani( Fusarium solani ), Fusarium fusae ( Fusarium sickle-shaped ), Alternaria ( Alternaria sp. ) or Curvularia spp. Curvularia spicifera ) and other plant root rot. Tests have shown that the biocontrol agent provided by the present invention has a 66.21% control effect on mung bean root rot and a 63.46% control effect on tobacco root rot under potted conditions.

The invention belongs to the technical field of microbial engineering and pharmaceutical engineering and particularly relates to alternarlactam extracted from a solid fermentation material of maubertiana boott endophytic fungi (Alternaria sp.) and a preparation method and anti-tumor effect thereof. The alternarlactam can be prepared by a solid fermentation method and a chemical synthesis method. The alternarlactam has the effects of strongly inhibiting the proliferation of human cervical cancer cells HeLa and human liver cancer cells QSG-7701, so that the alternarlactam can be used as a compound having the anti-tumor effect and is applied to preparing anti-tumor medicaments.