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Bacterial strain capable of degrading diuron and application of bacterial strain

A technology of diuron and bacterial strains, applied in the field of microorganisms, can solve problems such as adverse effects, achieve the effect of solving residue problems and improving the quality and safety of agricultural products

Active Publication Date: 2022-04-12
NINGXIA MEDICAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have shown that Diuron is an endocrine disruptor, which will have adverse effects on the human body after exposure

Method used

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  • Bacterial strain capable of degrading diuron and application of bacterial strain
  • Bacterial strain capable of degrading diuron and application of bacterial strain
  • Bacterial strain capable of degrading diuron and application of bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, isolation, purification and screening of bacterial strain QHSH-5

[0054] 1. Isolation and purification of strains

[0055] Diuron-contaminated soil was collected from Pingluo County, Shizuishan City, Ningxia in July 2020, and 2g was mixed in 50mL sterile water. After the solution was left to clarify, the supernatant was diluted to 10 by gradient dilution -1 、10 -2 、10 -3 、10 -4 、10 -5 Equal gradient, uniformly spread on commercial Martin solid medium, cultivate at 28°C, purify by plate streak separation method, and obtain multiple single purified microbial strains.

[0056] 2. Screening of diuron-degrading bacteria

[0057] Prepare commercial Martin's liquid medium (without adding agar), 250mL Erlenmeyer flask contains 150mL medium, inoculate multiple strains of single purification into the medium, 28°C, 180r / min shaking culture. After the strain grows to the logarithmic growth phase, the substrate Diuron is added at a concentration of 50mg / L, and the...

Embodiment 2

[0062] Embodiment 2, identification of bacterial strain QHSH-5

[0063] (1) Colony morphology

[0064] Inoculate QHSH-5 on a commercial Martin medium plate, the colony is round, dry, opaque, fluffy, with white hyphae on the surface. After the gray-white hyphae germinate in the initial stage, they spread to the medium in a circular manner, and after forming a circle, the middle color is gray-white and the periphery gradually becomes darker. (See figure 1 )

[0065] (2) Strain identification

[0066] Insert QHSH-5 into liquid commercial Martin medium, culture at 28°C, 180r / min, on a shaker for 3 days, and take a small amount of bacteria after centrifugation. Genomic DNA of QHSH-5 was extracted using the "Fungi Genomic DNA Extraction Kit" (Wuxi Biotek Biotechnology Co., Ltd., article number: B004009020), and the extraction steps were referred to the instructions of the fungal genomic DNA extraction kit. Using QHSH-5 genomic DNA as a template, PCR amplification was performed ...

Embodiment 3

[0075] Embodiment 3, the degradation assay of degrading bacteria QHSH-5

[0076] (1) Growth curve measurement

[0077] Take the slant preservation test tube where the degrading bacteria QHSH-5 is preserved, add 3mL liquid commercial Martin's medium, blow and suck repeatedly, and wash off the bacterial lawn, which is the mycelium eluate, and then transfer it to another sterile test tube with a straw , diluted 10 times with sterile water to make 10 mL of bacterial suspension. Configure 15 bottles of liquid commercial Martin’s medium, each 250mL Erlenmeyer flask contains 150mL of liquid commercial Martin’s medium, inoculate each bottle with 0.5mL of QHSH-5 bacterial suspension, and cultivate at 28°C and 180r / min constant temperature shaking. Set 3 groups in parallel, take samples on days 2, 3, 4, 5, 6, 7, 8, and 9, keep the mycelium, dry it to constant weight, weigh it, and measure the growth curve by dry weight method (see image 3 ). Growth curve such as image 3 As shown, ...

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Abstract

The invention discloses a strain capable of degrading diuron and application of the strain. The bacterial strain is an Alternaria sp. Bacterial strain QHSH-5, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No. 23258. The strain can be used for degrading pesticide diuron, is beneficial to solving the problem of agricultural diuron residues, and improves the quality safety of agricultural products.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a bacterial strain capable of degrading diuron and an application thereof. Background technique [0002] Diuron (Diuron) is a selective herbicide, hardly soluble in water and most organic solvents, chemically stable in the air, can be decomposed when encountering strong acid and alkali, non-flammable, non-corrosive, Molecular weight: 233.0945, molecular formula: C 9 h 10 Cl 2 N 2 O, the chemical structural formula is as follows: [0003] [0004] As my country has become a major pesticide producer, the production and use of Diuron in China has also grown rapidly. Diuron is used in cotton, corn, peanuts, sugar cane, and fruit trees; Annual weeds such as quinoa, also have a good control effect on perennial weeds, such as bermudagrass and Cyperus cyperi. At present, the demand for diuron for sugarcane in my country is about 1,500 tons. Due to the extensive use of diuro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C02F3/34B09C1/10C12R1/645A62D101/04A62D101/22A62D101/26A62D101/28C02F101/30C02F101/34C02F101/36C02F101/38
Inventor 吴秀丽王芳郑如亮马鹏生刘成周丽焦美娟
Owner NINGXIA MEDICAL UNIV
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