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44 results about "Horse chestnut" patented technology

Chestnut (horse anatomy) The chestnut, also known as a night eye, is a callosity on the body of a horse or other equine, found on the inner side of the leg above the knee on the foreleg and, if present, below the hock on the hind leg.

Method for extracting purified aescine from horse chestnut

The invention discloses a method for extracting purified aescine from horse chestnut, which comprises the following steps: carrying out reflow degreasing on raw materials by adopting diethyl ether, then putting the obtained dregs of a decoction in an extractor; adding ethanol into the extractor to carry out ultrasonic extraction; after the ultrasonic extraction is completed, carrying out centrifugal separation on the obtained product so as to obtain aescine extracting solution; carrying out decoloration on the aescine extracting solution by active carbons; carrying out microfiltration and ultrafiltration on the aescine extracting solution subjected to decoloration; concentrating the obtained filter liquor until the concentration of the filter liquor is lower than that of ethanol; carrying out adsorption on the obtained filte with water and alcohol; collecting aescine fractions and recycling reagent; and standing the aescine fractions for crystallization and carrying out recrystallization on the obtained product by acetone and aether. The method is suitable for scale-up production.
Owner:NANJING ZELANG MEDICAL TECH

Skin-care beatifying cream

The invention discloses a skin-care beatifying cream comprising deionized water, octadecanol, triple pressed stearic acid, hydrogenated lanolin, a non-ionic emulsifier, a natural essence, monoglyceride, a ginseng extracting solution, a horse chestnut extracting solution, a Chinese pearl barley extracting solution, a chrysanthemum extracting solution, an angelica root extracting solution, propylene glycol, an aloe extract, a novel amino-acid whitening agent, hyaluronic acid and propolis. The product of the skin-care beatifying cream has the advantages of mild texture, safety, reliability and no toxic or side effect, does not cause anaphylaxis, is suitable for all kinds of people, has the effects of promoting blood circulation of a skin surface layer, enhancing the functions of epidermis cells and enhancing the trophism, metabolism, tenderness, activity and elasticity of cells, achieves the purposes of moisturizing skin, deferring senility, softening cuticle, removing color fleck, enabling the skin to be tender, white and elastic, and preventing the occurrence of skin diseases, and has an obvious effect on improving skin diseases such as acnes, color flecks, freckles, chloasma and the like.
Owner:王宝忠

Eye mask with effects of relieving wrinkles and lightening dark eye circles

The invention discloses an eye mask with effects of relieving wrinkles and lightening dark eye circles. The eye mask is mainly prepared from the following components: horse chestnut extract, glucosylhesperidin and peony root extract. According to the eye mask, the effect of promoting generation of collagen and elastic fibers in the skin dermis can be achieved by utilizing two small-molecular active peptides, including palmitoyl tripeptide-5 and carnosine, and small-molecular hyaluronic acid, and the effects of improving skin moisture degree, improving skin elasticity, reducing skin wrinkles and relieving senescence can be achieved; the three plant extracts including horse chestnut extract, glucosyl hesperidin and peony root extract are matched to realize functions of promoting blood circulation of eyes, improving the luster of skin around the eyes, lightening the dark eye circles and reinforcing the product effects of lightening fine lines and resisting free radicals; and the mild performance and use comfortable feeling of the eye mask can be promoted by utilizing the characteristics of efficient preservation and low irritation of anions.
Owner:SHANGHAI YUEMU COSMETICS

Cultivation method of interplanting of horse chestnut and rivier giantarum rhizome

The invention discloses a cultivation method of interplanting of horse chestnut and rivier giantarum rhizome. The method comprises the steps that amorphophallus konjac varies which are locally planted for several years, can be better suitable for the local ecological condition and are good in disease resistance are selected, suitable horse chestnut woodland is selected, through a series of technical measures such as sowing at the right time, disinfection treatment of rivier giantarum rhizome seeds, scientific fertilization, comprehensive control of diseases and pests, and digging and harvesting through classification, advantages of the horse chestnut woodland are sufficiently developed, diseases and pests are lowered, and high yield and economic benefit are furthest obtained. Compared with the prior art, the rivier giantarum rhizome is planted under the horse chestnut, the horse chestnut can sufficiently use fertilizer and pesticide for planting the rivier giantarum rhizome again, the input of the fertilizer and pesticide is reduced, meanwhile, weeding is not needed, and the labor input is lowered; the horse chestnut creates the growth environment suitable for the rivier giantarum rhizome, the diseases of the rivier giantarum rhizome are reduced, the yield is improved, and the income is increased.
Owner:HANZHONG CITY AGRI TECH PROMOTION CENT

Planting method of horse chestnut

The invention provides a planting method of a horse chestnut and belongs to the field of tree planting. The method includes planting steps of planting field selection, spring seedling sowing and growing, sapling growing and seed collection. Compared with the prior art, the planting method has the advantages of adopting the high-efficiency multi-dimensional developing mode, conducting multilevel greening and protecting ecology. Quality of leaf buds, leaves, flowers and roots and seed output of the planted horse chestnut are improved due to the fact that plant diseases and insect pests of the horse chestnut are reduced and soil is table and nutrient.
Owner:南京宏冠生物科技有限公司

Simple process for obtaining beta-aescin from indian horse chesnut (aesculus indica)

InactiveUS20060030697A1Easy and convenient commercial extraction processThe process is simple and convenientSugar derivativesGlycosidesAqueous alcoholOrganic layer
The present invention relates to development of a simplified process for purification of β-aescin from Indian horse chestnut (Aesculus indica) by aqueous alcohol extraction, solventsolvent partition, treatment with base followed by passing the organic layer through acidic alumina in 2.0-3.0% yield of purity. minimum 90%.
Owner:COUNCIL OF SCI & IND RES

Method for preparing agent for preventing and treating red spider of Chinese chestnut using extract from vervain family flowers

InactiveCN101322503AAbundant resourcesThe direct extraction method is simpleBiocideAnimal repellantsDiseaseFruit tree
The invention relates to a method for prevention and control of diseases and insect pests in fruit trees, in particular to the method for preparing a medicament for prevention and control of spider mites on chestnut by using an extract from flower of vitex negundo. The method comprises the following steps: the flower of the vitex negundo is arranged in a container, ethanol with the concentration of more than 75 percent is added in the container for extraction, the still placement is carried out for 10 to 14 hours, and the mixture is then filtered to remove the residues, thereby preparing the medicament of the flower of the vitex negundo. The flower of the vitex negundo is widely distributed in the areas such as Hebei, Shanxi, Henan, Shanxi, Gansu, Sichuan and Yunnan provinces and is a wild plant, the resources are abundant, the direct extraction method by using the ethanol is simple and the extract is safe to human and livestock. As the medicament contains a great amount of volatile substances, the medicament has great potential as a plant acaricide.
Owner:吴桂军

Collagen peptide spray

The invention discloses a collagen peptide spray. The collagen peptide spray is prepared from the following components in parts by weight: water, butanediol, a rice fermentation product filtrate, a hydrolyzed sweet almond protein, a fomes officinalis extract, acetyl hexapeptide-8, glycine betaine, bio-saccharide gum-1, a coffee tree extract, 1,2-hexanediol, a mirabilis jasminum extract, arginine,N-Acetyl-D(+)-glucosamine, a horse chestnut extract, creatine, trehalose, 1,3-propylene glycol, dipotassium glycyrrhizinate, collagen peptide, caprylhydroxamic acid, a centella asiatica root extract,a scutellaria baicalensis root extract, a polygonum cuspidatum root extract, a tea extract, a chamomilla recutita flower extract, a liquorice root extract, a rosemary leaf extract, an organic iris extract, a false chinese swertia herb extract, jojoba oil and vitamin E. According to the collagen peptide spray, the fomes officinalis extract, the N-Acetyl-D(+)-glucosamine, hexopeptides and the hydrolyzed sweet almond protein are added, so that the moisturizing and skin-brightening effects and the skin tightening and improving effects for the instant feeling are good.
Owner:珠海水信生物科技有限公司

Euonymus japonicus endophytic bacterium bacillus methylotrophicus and application thereof

The invention discloses a strain of euonymus japonicus endophytic bacterium bacillus methylotrophicus HYEB 5-6 which was preserved in China Center for Type Culture Collection on Dec 8th, 2015, and the preservation serial number is CCTCC No: M2015728. The bacillus methylotrophicus HYEB 5-6 is an euonymus japonicus endophytic bacterium and can delay germination of euonymus japonicus anthrax conidium, inhibit extension of a germ tube, inhibit germination of the conidium, induce hypha to generate an expanded body and cause malformation of the hypha; it is proved by inoculation experiment results that after HYEB 5-6 thallus is sprayed, the index of decease infection of euonymus japonicus anthracnose is remarkably inhibited, and scabs of euonymus japonicus anthracnose are reduced; the HYEB 5-6 strain has a certain antagonistic effect on euonymus japonicus colletotrichum, alternaria spp, cytospora chrysosperma, higgins dish bacteria, magnaporthe oryzae, rhizoctonia, horse chestnut fusicoccin and fusarium solani.
Owner:NANJING FORESTRY UNIV

Hyaluronic acid BB cream with multiple effects and preparation method

InactiveCN107737062ASynergistically moisturizingBrighten skin colourCosmetic preparationsBody powdersDracaena dracoAdditive ingredient
The invention provides hyaluronic acid BB cream with multiple effects. The composition comprises the following ingredients by weight percent: 0.01-3.0% of hyaluronic acid, 1.0-5.0% of plant sucrose clear paste, 0.5-3.5% of a function compound and 89.0-98.4% of substrate auxiliary materials, wherein the plant sucrose clear paste is prepared by mixing eight active plants of an aloe smooth agent, herba leonuri, horse chestnut, lophatherum gracile, white willow barks, portulacae herba, dracaena draco linn and witch hazel and sucrose solution; the function compound is prepared by mixing nicotinamide, allantoin and polyglutamic acid at a ratio. The hyaluronic acid BB cream not only has the basic effects of covering, adjusting skin color, sunscreening, and refining pores, but also has the multiple effects of preserving moisture, brightening skin tone, being anti-allergy, diminishing inflammation, being antibacterial and anti-aging and the like; meanwhile, the hyaluronic acid BB cream is natural and simple in ingredients, excellent in water resistance and strong in setting capacity, so that the hyaluronic acid BB cream is a novel hyaluronic acid BB cream which is safe, practical, durable and stable and has the multiple effects.
Owner:杭州惠博士生物科技有限公司

Chinese herbal medicine fungicide suitable for pachyrhizua angulatus preservation

InactiveCN106889181ARich in flavonoidsHelps prevent discolorationFruit and vegetables preservationFungicideAdditive ingredient
The invention provides a Chinese herbal medicine fungicide suitable for pachyrhizua angulatus preservation. The Chinese herbal medicine fungicide is prepared from 9-15parts of honeysuckle flowers, 8-12 parts of dandelion, 8-10 parts of clove, 8-10 parts of horse chestnut, 8-10 parts of golden larch bark, 8-10 parts of nutmeg, 6-9 parts of radix stemonae, 6-9 parts of dalmatian insect flower, 3-6 parts of coptis chinensis, 6-9 parts of willow branches, 5-9 parts of fennel, 3-5 parts of Sichuan pepper, 5-9 parts of cassia bark, 6-9 parts of shaddock peel and 3-8 parts of broken-wall rape pollen. The safety performance of the obtained Chinese herbal medicine fungicide is high, and the fungicide has no harm to a human body. The pachyrhizua angulatus preservation effect is good by using the Chinese herbal medicine fungicide, and the preservation time in a pachyrhizua angulatus storage life can be very well prolonged.
Owner:FOSHAN HENGNAN MEDICAL TECH CO LTD

Multi-functional skin care essence and preparation method thereof

The invention relates to the technical field of cosmetics, and in particular relates to a multi-functional skin care essence and a preparation method thereof. The multi-functional skin care essence ismade from 60-90 parts of deionized water, 0.1-0.2 part of sodium hyaluronate, 0.1-0.2 part of hyaluronic acid, 0.15-0.2 part of algae extract, 0.15-0.25 part of chicory root extract, 1-3 parts of trehalose, 0.5-2 parts of palmitoyl pentapeptide-5, 1-2 parts of lecithin, 2-4 parts of amino acid, 2-4 parts of peony flower extract, 2-4 parts of horse chestnut extract, 2-4 parts of yeast extract, 3-5parts of beta-glucan, and 3-5 parts of N-carboxymethylchitosan. In the formula of the multi-functional skin care essence, the added various functional ingredients has good compatibility and synergetic interaction while cooperating with each other, and can focally regulate cells for each cell target on the skin, thereby greatly improving absorption and utilization rate of the product. The multi-functional skin care essence integrates multiple effects such as moisturizing, whitening and wrinkle-removing as a whole, and has long-lasting effect.
Owner:广东瀚森生物科技有限公司

Essence cream capable of preventing generation of striae gravidarum and preparation method of essence cream

The invention discloses essence cream capable of preventing generation of striae gravidarum. The essence cream contains efficacy ingredients from at least one of meadowfoam seed oil, butyrospermum parkii sheabutter, phytosterol, argania spinosa kernel oil, a yarrow extract, horse chestnut sapinogenin, a ruscus aculeata root extract, a centella asiatica extract and palmitoyl tripeptide-5. The efficacy ingredients in the essence cream disclosed by the invention can well exert synergistic effects, so that the essence cream can effectively prevent generation of the striae gravidarum, and has goodeffect of lightening and repairing the striae gravidarum. The essence cream is safe, free from toxic and side effects, convenient to use and low in price. Besides, the invention also discloses a preparation method of the essence cream.
Owner:广州睿森生物科技有限公司

Moisturizing lotion and preparation method thereof

The invention relates to moisturizing lotion, reasonably composed of horse chestnut extract, Siberian solomonseal rhizome extract, Dendrobium officinale extract, ginger root extract and other aids. The moisturizing lotion can provide comprehensive stereo moisturization for corium, epidermis, cuticle and skin surface, and has instant and long-acting moisturizing functions. The moisturizing lotion has multiple bioactivities, such as removing free radicals, resisting inflammations, and fighting skin aging; therefore, in addition to moisturizing, the moisturizing lotion may also act as a cosmeticmaterial with various bioactivities. A preparation method of the moisturizing lotion is simple, complex mechanical equipment is not required, and the cost is low.
Owner:绿之韵生物工程集团有限公司

Horse chestnut extract gel, as well as preparation method and application thereof

PendingCN110038036AReduce vasospasm irritationRelieves mucous membrane irritationOrganic active ingredientsAerosol deliveryAdditive ingredientIrritation
The invention discloses horse chestnut extract gel and a preparation method thereof. The effective ingredients of the horse chestnut extract preparation comprise aescine and L-arginine. According to the horse chestnut extract gel, the mucosal irritation of the horse chestnut extract is reduced by adopting aescine, and the horse chestnut extract gel directly acts on the local part with cervical erosion through locally external administration; and the horse chestnut extract is rich in hydroxyl, has strong moisture absorption, can be used for relieving local swelling of a patient to improve localedema to improve the compliance, and provides a new administration way to patients.
Owner:陕西三八妇乐科技股份有限公司

Eye mask liquid capable of removing dark circles, resisting aging and moisturizing and preparation method thereof

The invention belongs to the technical field of cosmetics, and particularly relates to eye mask liquid capable of removing dark circles, resisting aging and moisturizing and a preparation method thereof. The eye mask liquid is mainly prepared from hyaluronic acid, phytosterol oleate, water-soluble ceramide, palmitoyl tripeptide-5, soluble collagen, a horse chestnut extract, retinyl palmitate, a chondrus crispus extract, bio-glycopeptide, polyglycerylmetharylate and propylene glycol and the like, has remarkable effects of removing dark circles, improving skin elasticity, reducing wrinkles and enhancing skin hydrature, and is less in irritation and high in safety.
Owner:惠州市好好爱日化用品有限公司

Tooth-strengthening toothpaste

The invention discloses tooth-strengthening toothpaste, which is prepared by mixing 10 to 20 weight parts of calcium carbonate, 10 to 15 weight parts of calcium bicarbonate, 1 to 2 weight parts of calcium hydrogen phosphate dihydrate salt, 20 to 30 weight parts of honeysuckle flower, 6 to 10 weight parts of horse chestnut extract liquor, 4 to 6 weight parts of citric acid, 4 to 6 weight parts of menthol crystal, 0.5 to 1 weight part of dimethyl silicone oil, 0.5 to 1.5 weight parts of sodium carbonate, 1 to 2 weight parts of spice, 0.5 to 1weight part of xylitol, 4 to 6 weight parts of cation guar gum and 30 to 40 weight parts of deionized water. The permanent tooth toothpaste has the characteristics of the conventional toothpaste in the market and has a tooth-strengthening effect.
Owner:LINKCONN ELECTRONICS

Method for grafting seedling culture of new horse chestnut variety

The invention relates to a method for grafting seedling culture of a new horse chestnut variety. The method mainly comprises a step (1) of selecting 1-2-year horse chestnut seedlings as grafting stocks; in 20-30 days before grafting, transversely cutting off the stocks at the positions of 15-20 cm on the ground, coating the stock with a stock treating liquid, and then using a preservative film to tighten sections; a step (2) of selecting middle parts of annual branches growing robustly and having good growth vigor as scions, wherein collected scion lengths are 30-50 cm, the scion lengths are controlled to be 3-5 cm, and the thickness is controlled to be 0.6-0.7 cm; dipping the scion treating liquid, and performing grafting while performing dipping; a step (3) of performing grafting: cutting off 0.1-0.2 cm of the upper portions of the stock sections and then instantly performing grafting with the scions through a tongue grafting method; a step (4) of performing wax sealing of the scions; and a step (5) of performing graded temperature management after the grafting. The method has the advantages that the seedling culture period is short, the grafting survival rate is high, the new variety grows quickly after grafting, and the stress resistance is high.
Owner:LIANYUNGANG FORESTRY TECHN GUIDANCE STATION

Seedling cultivation method for rosa cymosa Tratt.

The invention provides a seedling cultivation method for rosa cymosa Tratt., and relates to the technical field of planting of ornamental trees. The seedling cultivation method comprises selection of seed maternal plants, selection of cultivation seeds, selection and processing of a seedling cultivation nursery, sowing, transplanting of seedlings, and control on diseases and pests. The seedling cultivation method for rosa cymosa Tratt. guarantees seed germination, cultivation seed quality and the germination rate. The emergency rate of the seedlings of rosa cymosa Tratt. is high, the seedling survival rate is high, and thus the planting method saves cost and is friendly to environment. The rosa cymosa Tratt. has high output values and extremely high practicability.
Owner:WUHU OUBIAO AGRI DEV

Chinese chestnut growth promoting base fertilizer and preparation method thereof

The invention relates to a Chinese chestnut growth promoting base fertilizer which comprises cow manure, sheep manure, pig manure, a sulfur-based compound fertilizer, diammonium phosphate, urea, calcium superphosphate and borax, wherein the manure is decomposed thoroughly and fermented; and the other components are mixed. During use, the Chinese chestnut growth promoting base fertilizer is applied to a field by means of furrowing between ridges; by the use of cow manure, the water content of Chinese chestnut is reduced and the protein content of Chinese chestnut is increased; by use of sheep manure, the protein content of the Chinese chestnut is effectively increased; borax can promote Chinese chestnut trees to blossom and bear fruit early; and the other components supplement sufficient nutriments to the Chinese chestnut trees and lay a solid foundation for next abundant harvest.
Owner:TIANJIN JIXIAN LVSHUNXING FRUIT & VEGETABLE CULTIVATION PROFESSIONAL COOP

Preparation method of aescin

ActiveCN103130864AIncrease contentSolve the problem of low contentSugar derivativesSteroidsImpurityHorse chestnut
The invention provides a preparation method of aescin. According to the invention, the fruit of horse chestnut is adopted as a raw material, and is subjected to crushing, extracting, concentrating, resin absorbing, and impurity eluting, such that aescin is obtained. Compared with prior arts, the method provided by the invention is simple and is easy to operate. Aescin content in first-time extraction can be higher than 20%, and the aescin content in the entire extraction process is gradually improved. After final extraction, the content can be higher than 88%. According to the invention, aescin extraction is carried out with limited raw material source, and a problem of low aescin content is solved. The method is suitable for industrialized productions.
Owner:南京宏冠生物科技有限公司

Chinese chestnut vinegar

The present invention relates to health-caring vinegar, in particular to Chinese chestnut vinegar which is produced with Chinese chestnuts as main material assisted by broomcorn, wheat bran and rice chaff according to the traditional vinegar production technique. The weight portions of the materials are as follows: 35 to 45 portions of granulated Chinese chestnuts, 25 to 35 portions of broomcorn,15 to 25 portions of rice chaff and 15 to 25 portions of wheat bran. The production method of the vinegar is as follows: according to the total weight of the materials, 45 percent of water is added into the materials and mixed; the mixture is then steamed in a steamer for one hour; the mixture is taken out and spread out to be cooled until the temperature is decreased to 40 DEG C; then, the mixture is added with saccharifying enzyme and saccharified and fermented for 25 to 28 days until thick material is produced; and according to the weight of the thick material, 40 percent to 50 percent of cool boiled water is added into the thick material; and after sprinkling and sterilization, the Chinese chestnut vinegar is produced. The present invention adopts trashed Chinese chestnuts-substandard small Chinese chestnuts-produced in Qianxi as material and then broomcorn, rice chaff, wheat bran and the like are added to produce the health-caring vinegar, which has the advantages of low materialcost and simple method. The edible vinegar produced with Chinese chestnuts is characterized by rich nutrition, sweetness, good taste and spleen invigoration.
Owner:于秀珠

Lotion based on cornua cervi pantotrichum extract and preparation method thereof

The invention discloses a lotion based on cornua cervi pantotrichum extract and a preparation method thereof, the lotion is prepared by the method as follow: preparation of cosmetic raw materials, extraction of the cornua cervi pantotrichum extract, extraction of rose essential oil, extraction of horse chestnut extract concentrate, extraction of gentian extract concentrate, extraction of eyebrightextract concentrate, extraction of water lily extract concentrate, extraction of propolis extract concentrate, extraction of papaya enzyme extract concentrate, extraction of ginseng extract concentrate, and extract treatment and fusion, the cosmetic raw materials comprise by weight: water, cornua cervi pantotrichum, hydrolyzed yeast protein, peony root and bark extract, tremella, giant typhoniumrhizome, radix angelicae, milk, the rose essential oil, common perilla, the horse chestnut extract concentrate, the gentian extract concentrate, aloe vera gel, the eyebright extract concentrate, the water lily extract concentrate, the propolis extract concentrate, the papaya enzyme extract concentrate and the ginseng extract concentrate, the raw materials of the lotion based on the cornua cervi pantotrichum extract are extracted from pure plants, and the lotion is mild, non-irritating, anti-inflammatory, analgesic, and itching-relieving, has the effect of promoting blood circulation to removeblood stasis, and is suitable for sensitive skin.
Owner:王风云

Gastrodia elata seed seedling culture nutritional medium

The invention belongs to the field of gastrodia elata plantation, and particularly relates to a gastrodia elata seed seedling culture nutritional medium. The medium comprises Chinese chestnut leaves,small horse chestnut leaves, oak leaves and orchidaceae symbiotic bacteria, and the weight ratio of Chinese chestnut leaves to small horse chestnut leaves to oak leaves is 3:4:3. A preparing method ofthe medium includes the steps of mixing the Chinese chestnut leaves, small horse chestnut leaves and oak leaves according to the weight ratio of 3:4:3 and conducting fermentation for 2-3 months at the temperature of 15-25 DEG C and the humidity of 50-70%. The germination rate of orchidaceae seeds can be effectively increased, and the rapid growth of orchidaceae plants is promoted.
Owner:保山华大智慧农业科技股份有限公司

Cosmetic composition for caring eye skin and preparation method thereof

The invention provides a cosmetic composition for caring eye skin, and aims to provide a cosmetic composition capable of effectively solving problems of dark circles, under-eye puffiness, fine lines and the like of the eye skin. The technical scheme of the invention is that: the cosmetic composition comprises: 0.1 part to 5 parts of plant extracts, 0.5 part to 3 parts of silanol and 0.05 part to 0.3 part of vitamin; the plant extracts include a sambucus nigra flower extract and a horse chestnut extract; and the cosmetic composition belongs to the technical field of a skin care product.
Owner:GUANGDONG BAWEI BIOLOGICAL TECH CO LTD

Composition for prevention and assisting treatment of haemorrhoids

The invention relates to a composition for prevention and assisting treatment of haemorrhoids. The composition comprises witch hazel extract and horse chestnut extract, and further comprises a bactericide, a humectant, a pH regulating agent and other substances. The composition can be used for preparing medicines or healthy products for prevention and / or assisting treatment of haemorrhoids.
Owner:DEMOTECH INC

Chinese chestnut blight nest-type PCR (polymerase chain reaction) detection kit and use method thereof

The invention discloses a Chinese chestnut blight nest-type PCR (polymerase chain reaction) detection kit and a use method thereof. The detection kit provided by the invention comprises (1) 10*PCR Buffer, wherein the components of the 10*PCR Buffer comprises 100 mmol / L<-1> of Tris-HCl at PH 8.3 and 500 mmol / L<-1> of KCl; (2) 2.5 mmol / L<-1> of dNTP; (3) 25 mmol / L<-1 > of MgCl2; (4) 5U / Mu L<-1> of Taq enzyme; (5) 10 Mu mol / L<-1> of primer ITS1 / ITS4, wherein the sequence of the ITS1 is 5'-TCCGTAGGTGAACCTGCGG-3' and the sequence of the ITS4 is 5'-TCCTCCGCTTATTGATATGC-3'; (6) 10 Mu mol / L<-1 > of premier ITSP1 / ITSP2, wherein the sequence of the ITSP1 is 5'-CCAGATACCCTTTGTGAACT-3' and the sequence of the ITSP2 is 5'-TCAGAGTCTTAGCGAGCC-3'; (7) sterilized double distilled water. According to the invention, the ITS1 / ITS4 and the ITSP1 / ITSP2 are adopted to form the nest-type PCR, 3 f g / Mu L<-1> genome DNA can be detected and the sensibility is increased by 1000 times. According to the adoption of the detection kit provided by the invention, pathogenic bacterium can be accurately detected in Chinese chestnut naturally pathogenetic tissues with the bacterium.
Owner:SICHUAN AGRI UNIV

Toxin-expelling blood-activating slimming cream

InactiveCN109419696APlay a role in eliminating toxinsPlay a role in blood warmingCosmetic preparationsToilet preparationsStearic acidHydroxybenzoate
The invention belongs to the field of slimming products and particularly relates to toxin-expelling blood-activating slimming cream, characterized by comprising, by weight, 3 parts of stearic acid, 1part of triethanolamine, 8 parts of sunflower oil, 4 parts of horse chestnut extract, 1 part of kola nut extract, 4 parts of arnica ethylene glycol extract, 5 parts of isopropyl myristate, 6 parts ofcetyl alcohol, 1 part of methyl 4-hydroxybenzoate, 8 parts of glycerol monostearate, and 60 parts of distilled water. The toxin-expelling blood-activating slimming cream also includes 2 parts of perilla herb extract, as well as 3 parts of argy wormwood leaf extract. The toxin-expelling blood-activating slimming cream provided herein can permeate the skin to act on the same so as to consume deep accumulated fat, can expel toxins and activate blood to warm the interior.
Owner:陈丹娜
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