97 results about "Entomopathogenic fungi" patented technology

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof.

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof.

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 3 and mutated sequences thereof.

The present invention utilizes fungal spore mass or hyphal fragments in burlap bags or sacks filled with biodegradable materials. The fungi may include saprophytic fungi, including gourmet and medicinal mushrooms, mycorrhizal fungi, entomopathogenic fungi, parasitic fungi and fungi imperfecti. The fungi function as keystone species, delivering benefits to both the microsphere and biosphere. Such fungal delivery systems are useful for purposes including ecological rehabilitation and restoration, preservation and improvement of habitats, bioremediation of toxic wastes and polluted sites, filtration of agricultural, mine and urban runoff, improvement of agricultural yields and control of biological organisms.

The present invention utilizes the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect attractants and / or pathogens. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material, attracting the insect and optionally introducing insect-specific pathogenic fungi. More than one fungus and substrate can be used in combination. The matrix of preconidial fungi can optionally be dried, freeze-dried, cooled and / or pelletized and packaged and reactivated for use as an effective insect attractant and / or biopesticide. Attractant extracts of the preconidial entomopathogenic mycelium are disclosed.

Disclosed herein are pest controlling compositions (i.e., biopesticides) comprising one or more entomopathogenic fungi which. Further disclosed are methods of using such compositions for controlling invasive pests, particularly agriculturally relevant pests.

The present invention utilizes extracts of the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect and arthropod attractants and / or pathogens and can be employed to limit the zoonotic diseases they transmit. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material and extracts can be made thereof. More than one fungus and substrate can be used in combination with one or more antimicrobial, antiprotozoal, antiviral, and genetically modified agents that result in reduced spread of contagions and lessens the damage they inflict on animals, and plants.

The present invention utilizes fungal spore mass or hyphal fragments in landscaping cloths, fiber substrates, paper products, hydroseeders and agricultural equipment. The fungi may include saprophytic fungi, including gourmet and medicinal mushrooms, mycorrhizal fungi, entomopathogenic fungi, parasitic fungi and fungi imperfecti. The fungi function as keystone species, delivering benefits to both the microsphere and biosphere. Such fungal delivery systems are useful for purposes including ecological rehabilitation and restoration, preservation and improvement of habitats, bioremediation of toxic wastes and polluted sites, filtration of agricultural, mine and urban runoff, improvement of agricultural yields and control of biological organisms. The invention allow for a variety of methods and products including the use of cardboard boxes as a delivery system for fungi with or without the combination with plant seeds for starting gardens, for controlling insects, or for the process of ecological recovery.

The present invention provides a novel Bacillus amyloliquefaciens strain that exhibits broad antifungal activity. The present invention also provides the use of the Bacillus amyloliquefaciens strain or an antifungal composition comprised of the novel strain for control of a broad range of fungal plant pathogens.

The present invention utilizes extracts of the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect and arthropod attractants and / or pathogens and can be employed to limit the zoonotic and plant diseases they transmit. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material and extracts can be made thereof. More than one fungus and substrate can be used in combination with one or more antimicrobial, antiprotozoal, antiviral, or genetically modified agents that result in reduced spread of contagions and lessens the damage they inflict on animals and plants.

Relating to technical field of microorganisms, the invention discloses a beauveria bassiana XNBb-04 strain and a culture method thereof. The beauveria bassiana XNBb-04 strain is preserved in China Center for Type Culture Collection on March 6, 2014, and the preservation number is CCTCC M 2014075. The beauveria bassiana XNBb-04 strain provided by the invention belongs to Deuteromycota, Moniliales, Moniliaceae, Beauveria. The beauveria bassiana XNBb-04 strain is a native entomopathogenic fungus of China, can be further used for preparation of living biopesticides, has good pathogenic effect on nodulated alfalfa weevils, can effectively kill nodulated alfalfa weevils, has brand new action mechanism different from existing chemical insecticides, and has no environmental pollution or residue. The beauveria bassiana XNBb-04 strain provided by the invention can be applied to various ecological environments in terms of pest control, and is conducive to environmental protection.

The invention discloses a traditional Chinese medicine washing agent used for fruits and vegetables. The traditional Chinese medicine washing agent is prepared via mixing of 40.0 to 60.0 parts of a traditional Chinese medicine extract, 2.0 to 3.0 parts of an emulsifying solubilizer, 4.0 to 6.0 parts of a surfactant, 0.1 to 0.2 part of disodium EDTA, 1.0 to 2.0 parts of sodium chloride, 0.06 to 0.1 part of isothiazolinone,50 to 65 parts of purified water; wherein the traditional Chinese medicine extract is prepared by taking radix sophorae flavescentis, lonicera japonica, fructus forsythia, herba schizonepetae, scutellaria baicalensis, cordate houttuynia, flos chrysanthemi, and pericarpium citri reticulatae as raw bulk drugs. The traditional Chinese medicine washing agent possesses excellent inhibition and killing effects on a plurality of bacteria and pathogenic fungi, and excellent degreasing performance, insect repellent performance, and biological degradability; is capable of removing dirt and pesticide residues on fruit and vegetable surfaces, and accords with life concept of modern people on green and environmental-friendly life.

The invention discloses an Isaria javanicanicus mould strain and application thereof. The high-efficiency Isaria javanicanicus (Friedrichs & Bally) strain Ij01 is classified to belong to Corynespora, Hyphomycetaceae, Hyphomycetales, Hyphomycetes, Mitosporic Fungi, Eumycota. The strain has been preserved in China Center for Type Culture Collection (CCTCC), the preservation date is Oct 27, 2008, and the preservation number is CCTCC M 208171. The high-efficiency Isaria javanicanicus mould is entomopathogenic fungi to have high efficiency for preventing and controlling prodenia litura, has the characteristics of brand-new action mechanism different from the prior chemical insecticides, and no environmental pollution and residue when taken as a living biological pesticide, and meets the requirement of organic food production.

Disclosed herein are pest controlling compositions (i.e., biopesticides) comprising one or more entomopathogenic fungi which. Further disclosed are methods of using such compositions for controlling invasive pests, particularly agriculturally relevant pests.

Disclosed are peptide and DNA compositions surprisingly found to be effective in generating immune responses against the pathogenic fungi Coccidioides spp., the causative agents of coccidioidomycosis and Valley Fever. The invention thus provides peptides and DNA constructs, combinations and related biological compositions, and prophylactic and therapeutic methods of using such components to generate effective immune responses against Coccidioides spp., including C. immitis.

Microsclerotia of entomopathogenic fungi, including Metarhizium species, Beauveria species, and Lecanicillium species, may be produced. These microsclerotia are effective for the control of a wide variety of insect pests, particularly soil-dwelling insect pests.

The present invention relates to the new use of an already known biomolecule methyl-beta-orcinol carboxylate of formula 1 isolated from a lichen (Everniastrum cirrhatum), for treating pathogenic fungal infections of humans that are resistant to polyene and azole antibiotics such as amphotericin B, nystatin, clotrimazole etc.

The invention discloses a high-efficiency Metarrhizium anisopliae strain and applications thereof. The strain MaB08 belongs to Eumycota, Mitotic spore fungi, Hyphomycetes, Hyphomycetales, Trichosporon and Metarrhizium, and is preserved in China Center for Type Culture Collection with the preservation date of October 27th, 2008 and the preservation number of CCTCC M 208172. The high-efficiency Metarrhizium anisopliae is an entomopathogenic fungus that has high efficiency for controlling brontispa longissima, and as a living biological pesticide, the high-efficiency Metarrhizium anisopliae has brand new action mechanism that is different from the existing chemical pesticides, has characteristics of non-pollution and non-residue to environment, and can fit for the requirements of organic food production.

The invention discloses an antibacterial detergent for tableware and a preparation method thereof. The method comprises the following steps: adding 0.5 t0 2 parts of traditional Chinese medicine concentrate, 8 to 12 parts of fatty alcohol polyoxyethylene ether sodium sulfate, 4 to 6 parts of lauryl sodium sulfate, 2 to 4 parts of laurate dihydroxyethylamide, 4 to 7 parts of dodecylbenzene sulfonic acid, 0.5 to 1.5 parts of sodium hydroxide, 0.2 to 0.6 part of sodium chloride, 0.06 to 0.1 part of Kazon and 0.1 to 0.2 part of essence into 100 parts of water by weight, wherein the traditional Chinese medicine concentrate is a traditional Chinese medicine extracting solution (d is between 1.5g / ml and 1.8g / ml) which is extracted by taking 85 to 95 parts of isatis root, 15 to 25 parts of mentha haplocalyx and 10 to 20 parts of flos caryophylli as raw materials and water as an extracting solution. The antibacterial detergent has the function of inhibiting various pathogenic fungi and can be used for rapidly removing various food blots and greases. The tableware cleaned by using the antibacterial detergent is free of stains.

The present invention relates to a method for manufacturing a fungal granule for controlling insects. The invention provides a method for preparing an entomopathogenic fungi sodium alginate granule and a granular formulation prepared through the method. Compared with the prior art, the invention not only preserves the normal advantages of long-time shape keeping of algae colloidal particle and growth recovering of fungus in the particles, but also has other innovations and improvements on other aspects. The algae colloidal particles containing fungus are used for being sprinkled to the farmland or woodland for controlling insects.

The disclosure provides compositions and dosage forms for promoting healthy microbial flora in a mammal, for example, a human. The disclosure further provides compositions and dosage forms for disrupting biofilm or preventing formation of biofilm containing pathogenic bacteria and / or pathogenic fungi in a particular region of a subject. The dosage forms contain (i) an isolated non-pathogenic fungal strain that is viable in the region of the subject, (ii) an isolated non-pathogenic bacterial strain that is viable in the region of the subject, and optionally (iii) an enzyme capable of disrupting the biofilm. The present disclosure also provides methods of disrupting a biofilm or preventing formation of a biofilm with such a composition or dosage form, methods of identifying a subject suitable for treatment with such a composition or dosage form, and methods of improving nutrient absorption using such a composition or dosage form.

Described herein is a method for blastospore-based insect control products of entomopathogenic fungi including either B. bassiana or / . fumosowsea that produces high concentrations of stable, effective spores by identifying nutritional and environmental conditions required for the rapid production of high concentrations of a stable and infective yeast-like blastospore composition.

Barrier treatment compositions using conidia of entomopathogenic fungi and methods for applying and using the same for bed bug population control and prevention are disclosed. The compositions and methods of the invention provide residual biopesticides control for bed bug populations using barrier treatment compositions transferring conidia of entomopathogenic fungi to a population of bed bugs providing effective kill within about 24 hours to about 10 days. The compositions and methods provide significantly enhanced horizontal transmission of the conidia of the entomopathogenic fungi providing enhanced bed bug control without detrimental effects of bed bug tolerance, resistance and / or negative impact on humans or animals contacting the residual biopesticides.

The invention discloses application of evodiamine and rutaecarpine in preparation of a medicine for enhancing the activation of NLRP3 inflammasomes. The evodiamine and the rutaecarpine can generate active Caspase-1 by enhancing activiation of macrophage inflammasomes so as to release manure inflammatory mediators such as IL-1beta and promote pyroptosis of sensitized macrophages. The activity of the evodiamine and the rutaecarpine can be used for enhancing the inflammatory response intensity of a human body and treating related diseases of insufficient activation of inflammasome including pulmonary tuberculosis and oral tuberculosis caused by intracellular infection of mycobacterium tuberculosis, diseases caused by listeria monocytogenes, related diseases caused by other intracellular infection bacteria, related diseases caused by pathogenic fungal infection, furunculosis, dental-root periapical diseases, chronic gastritis, chronic enteritis, dysentery and diarrhea and the like.

The invention discloses a highly pathogenic biocontrol bacteria Cordyceps javanica and application thereof. The study finds that Cordyceps javanica has strong insecticidal and insecticidal effects onbemisia tabaci and citrus sinensis, a highly pathogenic Cordyceps javanica strain GZQ-1 is screened and deposited in the Guangdong Provincial Microbial Culture Collection Center on the 20th of August,2018, and s strain collection number is GDMCC No: 60437. The strain is entomopathogenic fungus, can be used as a living biological pesticide, and has very strong application potential in the biological control of the bemisia tabaci and the citrus sinensis and on the disease control caused by germ spread of the bemisia tabaci and the citrus sinensis.

The invention provides an entomopathogenic fungi transgenic strain which contains a homologous or heterologous acidic trehalase constitutive exogenous gene. The preparation method of the entomopathogenic fungi transgenic strain comprises the following steps: firstly building an acidic trehalase full-length cDNA gene constitutive expression vector; establishing a submerged conidia transformation system; and finally selectively screening to obtain the entomopathogenic fungi transgenic strain. The invention also provides application of Entomopathogenic fungi acidic trehalase transgenic strain in preparing fungal pesticide. The entomopathogenic fungi transgenic strain has high efficiency of expressing trehalase; and the preparation method has the advantages of easy operation, good repeatability and good reproducibility. The entomopathogenic fungi transgenic strain can be applied to preparing the fungal pesticide. When the fungal pesticide acts on insects, through the overexpression of the acidic trehalase gene via the transgenic strain, the activity of acidic trehalase in an insect host is improved, utilization of the trehalase is accelerated and growth of entomopathogenic fungi in the insect host is promoted, thus effectively improving the insecticidal activity of the fungal pesticide such as metarhizium anisopliae, beauveria bassiana and the like.

The invention relates to antimicrobial peptide and application thereof, and aims to provide a defensin gene of antimicrobial peptide of bemisia tabaci (Gennadius), the antimicrobial peptide encoded by the defensin gene and a preparation method for the defensin gene. A nucleotide sequence of the gene of the antimicrobial peptide is shown as SEQ ID NO.1; and an amino acid of the antimicrobial peptide is shown as SEQ ID NO.2. A protease Xa factor is introduced into an expression vector, so that recombinant fusion protein can be effectively digested, and defensin polypeptide is obtained through rapid separation in an ultrafiltration mode according to the magnitude of protein molecules; the whole process is quick and simple; and the obtained antimicrobial peptide has antifungal activity, and can remarkably inhibit the growth of entomopathogenic fungi such as Beauveria bassiana, Metarhizium anisopliae, Botrytis cinerea and the like.

The invention provides an entomopathogenic fungus conidium collection process. An entomopathogenic fungus strain is inoculated into Sabouraud liquid medium and cultured, so that an entomopathogenic fungus liquid strain is obtained; the liquid strain is then inoculated into rice medium or bran medium and cultured until spore production are mature; after being mixed, the medium containing the mature spore production and vegetable oil or mineral oil are uniformly stirred under the process temperature of 3 DEG C to 30 DEG C, so that a dispersed system is obtained; the dispersed system is put onto a 120mu m to 150mu m screen and filtered, after obtained filtrate is dewatered under vacuum condition and then centrifugally filtered or filtered by screening, conidium ointment is collected. The conidium ointment which is collected by the screening process combined with stirring under the specific temperature (3 DEG C to 30 DEG C) not only can prevent conidia from being damped, but also can be directly used for preparing pesticide products, such as powder and suspension.

Disclosed herein are pest controlling compositions comprising entomopathogenic fungi which are horizontally transmissible across pest populations and control target pests at various life stages. Further disclosed are methods of using such compositions for controlling pests, including, bed bugs and other invasive parasitic pests.

The present invention provides insecticidal lipids and lipid fractions from fungi of the phylum Ascomycota, together with compositions comprising such lipids and lipid fractions and methods of preparing same. Methods for the biological control of insects, such as phytopathogenic insects, using the lipids and lipid fractions or compositions comprising said lipids optionally together with one or more insecticidal or entomopathogenic agents including entomopathogenic fungi, are also provided.