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Entomopathogenic fungus conidium collection process

A technology of pathogenic fungi and conidia, which is applied in the field of conidia collection technology, can solve the problems of entomopathogenic fungi conidia flying around, affecting the air quality in the operating area, and endangering the health of personnel, so as to avoid air pollution and endanger the operation The health of personnel, the effect of enhancing the insecticidal effect, and improving the recovery rate

Pending Publication Date: 2017-07-14
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sub-sieving method or the cyclone collection method are mainly used to collect the conidia of entomopathogenic fungi and obtain spore powder. Using these two methods will easily cause the conidia of entomopathogenic fungi to fly around, which will affect the air quality in the operating area and even endanger the health of personnel.
In addition, spore powder needs to be stored in a dry environment, which is inconvenient to store

Method used

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  • Entomopathogenic fungus conidium collection process
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Examples

Experimental program
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Effect test

Embodiment 1

[0021] Conidial culture of Metarhizium anisopliae, the CQMa421 strain of Metarhizium anisopliae was inoculated on a 1 / 4 strength Savoy liquid medium and cultured at 26°C for 3 days to obtain the liquid strain of Metarhizium anisopliae; The liquid strain was inoculated on the sterilized rice culture medium (the rice was soaked in tap water at 26°C for 4 hours, then drained, sterilized and cooled to room temperature to prepare the rice culture medium), and the rice culture medium was placed Fermented at 26°C for 15 days until the sporulation matures. The conidia of Metarhizium anisopliae were collected, and the rice medium containing mature spores and soybean salad oil were mixed at a weight ratio of 1:2.5 (100Kg of mature spore-forming rice medium and 250Kg of soybean salad oil) at 15°C Under the process temperature, stir at 20 rpm for 10 minutes to obtain a dispersion system; place the aforementioned dispersion system on a 120 um screen and filter, and first use a vacuum dryer ...

Embodiment 2

[0023] Conidial culture of Metarhizium anisopliae, inoculate the CQMa102 strain of Metarhizium anisopliae on a 1 / 4 strength Savoy liquid medium and culture it at 27°C for 4 days to obtain the liquid strain of Metarhizium anisopliae; The liquid strain was inoculated on the sterilized rice culture medium (the rice was soaked in tap water at 27°C for 4 hours and then drained, then sterilized and cooled to room temperature to prepare the rice culture medium), and the rice culture medium was placed Fermented at 27°C for 15 days until the spores mature. Conidia of Metarhizium anisopliae were collected, and the rice medium containing mature spores and soybean salad oil were mixed at a weight ratio of 1:4 (100Kg of mature spore-containing rice medium and 400Kg of soybean salad oil) at 17°C Under the process temperature, stir at 20rpm for 10 minutes to obtain a dispersion system; place the aforementioned dispersion system in a 150um screen and filter, and the obtained filtrate is first ...

Embodiment 3

[0025] For conidia culture of Beauveria bassiana, the CQBb101 strain of Beauveria bassiana was inoculated on a 1 / 4-strength Savoy liquid medium and cultured at 25°C for 3 days to obtain a liquid strain of Metarhizium anisopliae; The aforementioned liquid strains were inoculated on the sterilized wheat bran medium (the wheat bran and tap water were mixed according to the weight ratio=2:1, then mixed well, and then sterilized and cooled to room temperature to prepare the wheat bran medium). The wheat bran medium was fermented at 25°C for 15 days until the sporulation matured. The conidia of Beauveria bassiana were collected, and the wheat bran medium containing mature spores and rapeseed oil were mixed at a weight ratio of 1:5 (100Kg rice medium containing mature spores and 500Kg rapeseed oil). Under the process temperature of ℃, stir at 5 rpm for 10 minutes to obtain a dispersion system; place the aforementioned dispersion system on a 120 um screen and filter, and use a vacuum d...

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Abstract

The invention provides an entomopathogenic fungus conidium collection process. An entomopathogenic fungus strain is inoculated into Sabouraud liquid medium and cultured, so that an entomopathogenic fungus liquid strain is obtained; the liquid strain is then inoculated into rice medium or bran medium and cultured until spore production are mature; after being mixed, the medium containing the mature spore production and vegetable oil or mineral oil are uniformly stirred under the process temperature of 3 DEG C to 30 DEG C, so that a dispersed system is obtained; the dispersed system is put onto a 120mu m to 150mu m screen and filtered, after obtained filtrate is dewatered under vacuum condition and then centrifugally filtered or filtered by screening, conidium ointment is collected. The conidium ointment which is collected by the screening process combined with stirring under the specific temperature (3 DEG C to 30 DEG C) not only can prevent conidia from being damped, but also can be directly used for preparing pesticide products, such as powder and suspension.

Description

Technical field [0001] The invention relates to a collection process of conidia, which is used for collecting conidia of insect pathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae. Background technique [0002] Beauveria is a Deuteromycete, Neurospora, Neurosporidae, Fungi, Metarhizium, Ascomycota, Hypocrea, Ergotaceae, Fungi, Beauveria and Metarhizium are The most representative insect pathogenic fungus. [0003] Entomopathogenic fungi are widely parasitic on grubs, houseflies, scale insects, whitefly, aphids, thrips, potato leaf beetles, cockroaches, locusts, grasshoppers, crickets, boll weevil, cotton bugs, corn borers, longhorn beetles, and sugarcane Insects such as scarabs can cause disease to death. The pathogenic principle is: conidia (also known as spores) produced by insect pathogenic fungi in the process of asexual reproduction invade the insect (host) body and pass Insects (hosts) continue to grow and reproduce in their bodies to consume the nutrient...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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