The invention discloses a seedling substrate for garden plants and a preparation method of the seedling substrate. The seedling substrate is prepared from the following raw materials: saw dust, corncob, soybean straw, peanut shells, pine sawdust, cedarwood dust, distillers' grains, pyrethrum straw, bagasse, rice husk, peat soil, vermiculite, medical stone, lintonite, sphagna, carbendazim, cow dung, a zymophyte solution, inorganic fertilizers, gentle willow twigs, pine needles, tobacco leaves, wormwood, camphor leaves, agastache rugosus leaves and water. The seedling substrate for the garden plants, which is disclosed by the invention, is not only rich in various plant active substances and beneficial to growth and development of seedlings, but also very good in disease resistance, the insect expelling effect can be maintained for a long time, no pesticide product is added, soil and ambient environments can be protected, the seedling substrate can be very well fused with the soil and the environment after a certain time, adverse influence caused by pesticides can be reduced, prevention and treatment of insects and diseases in later management can be facilitated, and labor cost and economic cost of daily maintenance can be reduced.