Brevibacillus laterosporus for inhibiting riziocotinia solani, and application thereof

A technology of rice sheath blight and Brevibacterium, which is applied in the field of microbial control of rice sheath blight, and can solve the problem that there is no B. Dosage, etc.

Active Publication Date: 2017-09-12
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that although the domestic research on the biocontrol of Brevibacillus spp. for plant diseases has been carried out in recent years, the relevant research is still in the initial stage, and there is no microbial sterilization with Bacillus spp. as the main component. There is no relevant report about the inhibitory effect of Brevibacillus spp. on rice sheath blight

Method used

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  • Brevibacillus laterosporus for inhibiting riziocotinia solani, and application thereof
  • Brevibacillus laterosporus for inhibiting riziocotinia solani, and application thereof
  • Brevibacillus laterosporus for inhibiting riziocotinia solani, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Screening, isolation and identification of strains

[0027] Isolation and cultivation of bacterial strains: the medium is beef extract-peptone agar medium (NA): 3g of beef extract, 5g of peptone, 5g of sodium chloride, 20g of agar, 1000ml of distilled water, pH 7.0-7.2.

[0028] Separation by dilution coating method. Weigh 10g of newly collected soil (or the soil after drying in the shade), pour it into a triangular flask filled with 90ml of sterile water, shake it for 10min to form a suspension with a concentration of 1 / 10, bathe in 80°C water for 20min, and take 1ml of the supernatant solution, prepared as 10 -7 or 10 -8Concentration dilutions are available for use. Pour the melted NA into a sterilized petri dish. After solidification, draw 50 μl of the diluent and drop it on the surface of the solidified medium, spread it evenly with a sterile applicator stick, and place the petri dish upside down in a 28°C incubator for cultivation 1~2d. After the gr...

Embodiment 2

[0030] Example 2: Determination of the inhibitory effect of bacterial strain SNB10 on rice sheath blight pathogen by confrontation culture

[0031] Take a dish of rice sheath blight bacteria with a diameter of 8 mm and put it in the center of a petri dish filled with PDA. At a distance of 2 cm from the dish, use an inoculation ring stained with strain SNB10 to draw two parallel straight lines, and incubate at 28 ° C for 3 days , measure the width of the inhibition zone, and observe the growth of rice sheath blight mycelia in the inhibition zone by microscopic observation ( figure 2 ); continue to cultivate for 30 days until the rice sheath blight fungus produces sclerotia, and observe the persistence of the inhibitory effect. The test results showed that the strain SNB10 could strongly inhibit the growth of the rice sheath blight fungus, and microscopic observations showed that the hyphae of the rice sheath blight fungus were deformed and broken, the cell membrane changed, th...

Embodiment 3

[0032] Embodiment 3: the mensuration of antibacterial activity of bacterial strain SNB10 fermented liquid

[0033] Preparation of sterile fermentation filtrate: Transfer the strain SNB10 to fresh slant medium, culture it at 28°C for 1 day, inoculate it into a 250ml Erlenmeyer flask with a liquid volume of 50ml, rotate it at 28°C with a speed of 150r / min Vibrated on a shaker for 40 hours to obtain a fermentation stock solution, which was centrifuged at 10,000 r / min for 20 minutes and placed in a Filtrated in a bacterial filter to obtain a sterile fermentation filtrate to determine its antibacterial activity.

[0034] Fermentation medium: 3g beef extract, 5g peptone, 5g sodium chloride, 1000ml distilled water, pH 7.0-7.2.

[0035] The antibacterial activity of strain SNB10 aseptic fermentation filtrate on rice sheath blight was determined by the cup-and-disc method: the rice sheath blight bacteria dish with a diameter of 8 mm was placed upside down in the center of the PDA pla...

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Abstract

The invention discloses a strain of brevibacillus laterosporus SNB10 for inhibiting riziocotinia solani. The brevibacillus laterosporus SNB10 was preserved in China General Microbiological Culture Collection Center on May 17, 2017, and the preservation number is 14171. The brevibacillus laterosporus SNB10 has the following characteristics of high inhibition effect on the riziocotinia solani and good prevention and treatment effect on rice sheath blight when being prepared into a biocontrol aqueous solution. The brevibacillus laterosporus SNB10 can produce chitinase, is wide in antibacterial spectrum, and has a certain inhibition effect on various plant pathogenic fungi and pathogenic bacteria. The brevibacillus laterosporus SNB10 does not have toxicity and pathogenicity, is safe for human and livestock, environmentally friendly, high in stress resistance and stable and durable in bacteriostatic activity, and has potential application prospect. The brevibacillus laterosporus SNB10 provides a microbial strain and a pesticide product thereof, wherein the microbial strain is non-toxic for human and livestock and friendly for the ecological environment, and has a good effect of preventing the rice sheath blight.

Description

technical field [0001] The invention relates to the technical field of microbial control of rice sheath blight, in particular to a strain of Brevibacillus orthospora inhibiting rice sheath blight and its application. Background technique [0002] Rice sheath blight (Rhizoctonia solani) is one of the most serious and difficult fungal diseases at home and abroad. It is widely distributed and seriously damaged, causing huge economic losses to agricultural production every year. Due to the strong saprophytic ability and wide range of hosts of sheath blight, no high-resistance varieties have been found at home and abroad. At present, Jinggangmycin and some chemical pesticides are mainly used to prevent and control rice sheath blight in my country. The long-term repeated use of antibiotics and chemical agents has caused the pathogenic bacteria to produce obvious drug resistance, resulting in a decrease or even loss of control effect, and the dosage of pesticides continues to incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 赵秀香吴元华赵柏霞夏博安梦楠夏子豪
Owner SHENYANG AGRI UNIV
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