Compositions and methods for treating pests
A pest and pest killing technology, applied in the direction of botany equipment and methods, applications, biocides, etc., can solve the problems of not being reusable, losing effectiveness, etc.
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example 1
[0255] Example 1 : Mortality of adult bedbugs when exposed to different entomopathogens
[0256] An adult population of 50% males and 50% females was fed for five days prior to testing. This ensures that the insects are hydrated and their nutritional needs are met.
[0257] These insects were exposed to the strain Metarhizium anisopliae strain F52 (F52) and two strains of Beauveria bassiana: Beauveria bassiana ATCC 74040 (Bb40) and Beauveria bassiana ATCC 74250 (Bb50). One (1) mL dilution of each isolate was made in an aqueous solution of 0.05% Tween 80 and confirmed by a hemacytometer combined with germination counts to correspond to 1 x 10 6 , 1x 10 7 , 1x 10 8 , and 1x 10 9 Viable spores / mL. These 1 mL dilutions were pipetted into 6.0 cm by 1.5 cm Petri dishes (Whitman No. 1, 5 cm diameter) fitted with filter paper. Four (4) bed bugs (2 males and 2 females) were placed in each petri dish and allowed to crawl in the dish for 30 minutes (plate 1). Controls (Chk) were...
example 2
[0275] Example 2: Effects of Different Entomopathogens on Bedbug Eggs and Nymphs
[0276] Bedbug eggs were exposed to the strain Metarhizium anisopliae strain F52 (F52) and two strains of Beauveria bassiana: Beauveria bassiana ATCC 74040 (Bb40) and Beauveria bassiana ATCC 74250 (Bb50). Dilutions of each isolate were made in an aqueous solution of 0.05% Tween 80 and confirmed by a hemocytometer combined with germination counts to correspond to 1 x 10 in 0.05% Tween 80. 7 and 1x 10 8 Viable spores / mL. A solution of 0.05% Tween 80 was used as a control.
[0277] Four (4) bed bug eggs were placed in each 6.0 cm by 1.5 cm petri dish fitted with the 1.5 cm diameter filter paper. Petri dishes were replicated 5 times for each treatment. Shake the dilution well to suspend the microparticles in the test solution. A 0.25 ml sample of each concentration was pipetted into a 6.0 cm x 1.5 cm petri dish containing the eggs. The Petri dish was ventilated for 4 hours to allow excess moi...
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