Highly pathogenic biocontrol bacteria Cordyceps javanica and application thereof

A highly pathogenic and bio-control bacteria technology, applied in the direction of application, biocide, microorganisms, etc., can solve the problems of high toxicity, loss of biodiversity, excessive pesticide residues, etc., to achieve strong infection and insecticidal effect, strong application potential effect

Active Publication Date: 2019-02-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the pesticides are highly toxic and have been banned by the country, and the long-term and large-scale use of chemical pesticides has led to a series of problems such as the death of natural enemy insects, the loss of biodiversity, the generation of different degrees of drug resistance, and the pollution of the ecological environment. Problems also happen from time to time

Method used

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  • Highly pathogenic biocontrol bacteria Cordyceps javanica and application thereof
  • Highly pathogenic biocontrol bacteria Cordyceps javanica and application thereof
  • Highly pathogenic biocontrol bacteria Cordyceps javanica and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Isolation and Identification of Cordyceps Javanese Strain GZQ-1

[0029] 1. Source of materials

[0030] (1) Sample: Cordyceps javanica was preserved by the Biological Control Engineering Research Center of the Ministry of Education, South China Agricultural University. The preserved sample of this strain was purified by single cell isolation.

[0031] (2) Potato Dextrose Agar Medium (PDA): 200g peeled potatoes, cut into small pieces, add 1L of water, boil for 20min, filter with gauze: add 20g of glucose while hot, 18-20g of agar, stir to dissolve and mix, set Fill it into an Erlenmeyer flask and sterilize it in an autoclave (121°C, 30min) for 30min.

[0032] (3) Aseptic operating conditions: All utensils and utensils must be sterilized in a pressure cooker (121°C, 30min), and operations such as inoculation are performed in an ultra-clean workbench.

[0033] (4) Culture conditions: culture in a 25°C light (12L:12D) incubator, transfer to a PDA slope after th...

Embodiment 2

[0057] Example 2 Determination of the pathogenicity of Cordyceps javanica GZQ-1 strain to different ages of citrus psyllids

[0058] 1. Bioassay is one of the effective means to detect the lethality and lethality rate of entomogenic fungi to target pests, and can provide an important reference for comprehensive evaluation of the biological control potential of entomogenic fungi. In this study, the pathogenicity of Cordyceps javanica GZQ-1 strain to different ages of citrus psyllids was determined, in order to screen out the optimal age and concentration for its lethality to citrus psyllids.

[0059] 2. Insects and host plants for testing: citrus psyllids. Adults of citrus psyllids were raised in the net room of the Biological Control Engineering Research Center of the Ministry of Education, and inoculated on clean and healthy Jiulixiang. Each plant of Jiulixiang was inserted with about 20 pairs of adults. A net made of PVC film was used to cover each pot of Murata japonica, an...

Embodiment 3

[0082] Embodiment 3 Java Cordyceps GZQ-1 bacterial strain is to citrus psyllid adult field control

[0083] 1 × 10 with Cordyceps javanica GZQ-1 under field conditions 7 Spore / ml spore suspension was used to treat adult citrus psyllids, and Cordyceps fumosorosea strain IF010 and Metarhi ziumanisopliae strain CNGD7 were used as controls.

[0084] For its 13d cumulative survival rate, see image 3 , as the concentration of Cordyceps javanica spores increased, the lethality rate increased, and after 7 days of infection, the infection rate of Cordyceps javanica strain GZQ-1 was significantly higher than that of Cordyceps fumigatus strain IF010 and Metarhizium anisopliae strain CNGD7 (P <0.05).

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Abstract

The invention discloses a highly pathogenic biocontrol bacteria Cordyceps javanica and application thereof. The study finds that Cordyceps javanica has strong insecticidal and insecticidal effects onbemisia tabaci and citrus sinensis, a highly pathogenic Cordyceps javanica strain GZQ-1 is screened and deposited in the Guangdong Provincial Microbial Culture Collection Center on the 20th of August,2018, and s strain collection number is GDMCC No: 60437. The strain is entomopathogenic fungus, can be used as a living biological pesticide, and has very strong application potential in the biological control of the bemisia tabaci and the citrus sinensis and on the disease control caused by germ spread of the bemisia tabaci and the citrus sinensis.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a highly pathogenic biocontrol fungus Cordyceps javanica and its application. Background technique [0002] Insect damage refers to the damage caused by harmful insects to plant growth. Such as Bemisia tabaci (Gennadius) tomato, cucumber, pepper and other vegetables and cotton and many other crops, Bemisia tabaci directly sucks plant juice, resulting in plant weakness, nymphs and adults can also secrete honeydew, which induces sooty disease When the density is high, the leaves appear black, which seriously affects photosynthesis. In addition, Bemisia tabaci can also transmit more than 70 kinds of viral diseases on 30 kinds of crops. Different biotypes transmit different viruses, and the damage caused by them cannot be underestimated. Another example is the citrus psyllid (Diaphorinacitri Kuwayama), an important insect vector of citrus greening diseas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 邱宝利欧达阿里·肖卡特张利荷
Owner SOUTH CHINA AGRI UNIV
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