Beauveria bassiana XNBb-04 strain and culture method thereof

A technology of Beauveria bassiana and its culture method, applied in the field of Beauveria bassiana XNBb-04 strain and its cultivation, can solve the problem of green dairy industry, alfalfa root nodule larvae are difficult to work, and no Beauveria bassiana has been found Bacterial strains and other issues, to achieve the effect of environmental protection

Active Publication Date: 2015-12-23
XINJIANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of a large number of chemical pesticides not only pollutes the environment and alfalfa grass, which goes against the original intention of the development of the green dairy industry, but also is difficult to be effective against alfalfa root nodule larvae
The use of Beauveria bassiana to control Rhizobium meliloti is of great significance to control the increase of its population and reduce environmental pollution. In the aspect of biological pesticide control, no Beauveria bassiana strains have been found to control Rhizobium meliloti

Method used

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  • Beauveria bassiana XNBb-04 strain and culture method thereof
  • Beauveria bassiana XNBb-04 strain and culture method thereof

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Effect test

Embodiment 1

[0019] Example 1, the Beauveria bassiana XNBb-04 strain was deposited in the Type Culture Collection Center on March 6, 2014, and the preservation number is CCTCCM2014075.

Embodiment 2

[0020] Example 2, the morphological characteristics of the Beauveria bassiana XNBb-04 strain are as follows: the colony is white velveteen at the beginning, and then becomes milky yellow powder, and under the potato agar glucose medium culture conditions, the back of the colony is colorless or Yellow; conidiophores are short, colorless, without septa, the top extends in a zigzag shape, solitary or aggregated into spore bundles, the wall is smooth and transparent, the base of the phialide is spherical or quasi-elliptic and enlarged, the upper part is slender, the size is 96 μm to 142 μm × 4.9 μm to 7.3 μm, with an average size of 120 μm × 5.8 μm; conidia are spherical, oval, elliptical, colorless, single cell, with a size of 2.47 μm to 5.21 μm × 1.95 μm to 3.63 μm, The average size is 3.16 μm × 2.65 μm, without chlamydospores. as attached figure 1 shown.

[0021] Specifically, the isolation and acquisition method of Beauveria bassiana XNBb-04 bacterial strain of the pres...

Embodiment 3

[0062] Embodiment 3, the cultivation method of this Beauveria bassiana XNBb-04 bacterial strain, carries out according to the following steps:

[0063] The first step is to expand the cultivation of the first-level strains: put the XNBb-04 strain of Beauveria bassiana into a test tube equipped with PDA slant medium, put it into an incubator, and place it at a temperature of 24°C to 28°C and a relative humidity of Cultivate under the condition of 70% to 85%, and obtain the first-class strain culture after the hyphae cover the slope and produce spores;

[0064] The second step, secondary bacterial strain expansion cultivation: under aseptic conditions, scrape off the strain spore powder of the cultivated primary bacterial strain culture, and prepare a concentration of 5 × 10 with sterile water. 7 spore / mL suspension, suck 1mL and insert it into a 250mL Erlenmeyer flask filled with 50mL PDB culture solution, so that the spore concentration in the culture solution is 10 ...

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Abstract

Relating to technical field of microorganisms, the invention discloses a beauveria bassiana XNBb-04 strain and a culture method thereof. The beauveria bassiana XNBb-04 strain is preserved in China Center for Type Culture Collection on March 6, 2014, and the preservation number is CCTCC M 2014075. The beauveria bassiana XNBb-04 strain provided by the invention belongs to Deuteromycota, Moniliales, Moniliaceae, Beauveria. The beauveria bassiana XNBb-04 strain is a native entomopathogenic fungus of China, can be further used for preparation of living biopesticides, has good pathogenic effect on nodulated alfalfa weevils, can effectively kill nodulated alfalfa weevils, has brand new action mechanism different from existing chemical insecticides, and has no environmental pollution or residue. The beauveria bassiana XNBb-04 strain provided by the invention can be applied to various ecological environments in terms of pest control, and is conducive to environmental protection.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and relates to a Beauveria bassiana XNBb-04 strain and a cultivation method thereof. Background technique [0002] Alfalfa is the most important leguminous forage in the world. It is known as the "king of forage". Due to its strong adaptability, high grass yield, and rich in protein, it is widely cultivated in the world. In addition to being used as forage grass for livestock, planting alfalfa can also improve the soil and fertilize the field, improve the yield and quality of subsequent crops, and at the same time maintain water and soil and improve the ecological environment. Xinjiang is the hometown of alfalfa planting and one of the main production areas of alfalfa planting in my country. Alfalfa production is one of the important pillar industries for the development of animal husbandry in Xinjiang. [0003] Medicago clover nodule belongs to Coleoptera (Coleoptera), weevils (Curculion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01P7/04C12R1/645
Inventor 李克梅赵莉
Owner XINJIANG AGRI UNIV
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