42 results about "Beauveria amorpha" patented technology

The present invention relates to microencapsulated preparations comprising biopesticide, bio-fungicide, and plant growth promoting agents and uses thereof, as well as to methods for controlling pests and for producing said preparations for managing insect pests. In particular, the invention relates to microencapsulated preparations comprising at least one insect-pathogenic (entomopathogenic) fungus selected from Metarhizium spp. and Beauveria bassiana, and at least one pathogenic Bacillus spp. bacterial strain, preparations and particles comprising these, their use on plant material, methods for controlling pests therewith, and processes for their production.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The present application belongs to the technical field of preparation of white-backed caterpillar. More particularly, it relates to a Beauveria bassiana agent for greatly improving the time concentration of death of domestic silkworm and a high-quality white-backed caterpillar production method. The present application provides a Beauveria bassiana agent containing a strain of Beauveria bassiana and a fermentation product of another strain of Beauveria bassiana without bacteria bodies. The use of the agent in the present application to infect domestic silkworm can shorten the time of complete death of domestic silkworm, and concentrate the death time of domestic silkworm at the 6th day after inoculation, with a time span of only 24 hours, which helps to improve the production efficiency of white-backed caterpillar, effectively reduces the labor of feeding and picking silkworms during the death stage, and reduces the production cost. In addition, the method for producing white-backed caterpillar in the present application can achieve good infection effect by spraying the agent once, reducing the process of white-backed caterpillar production. And the white-backed caterpillar produced by the method in the present application is uniform in size, the consistency of product quality parameters is good, and the quality is stable, which helps to improve the clinical application effect.

The invention belongs to the technical field of pesticides, and particularly relates to a biopesticide for preventing and treating citrus red mites, the active component of the biopesticide is formed by compounding beauveria bassiana and cyflumetofen, or is formed by compounding beauveria bassiana and guadipyr; the number of spores in the beauveria bassiana is 5 billion spores per gram. The compounded combination of the biopesticide has a synergistic effect on the citrus red mites within a certain mass ratio range, especially when the compounding mass ratio of the beauveria bassiana to the guadipyr is 1: 30 and 1: 20, the co-toxicity coefficient reaches 418.133 and 317.078, the synergistic effect is remarkable, and the prevention and treatment effect on the citrus red mites can be improved. Besides, the biopesticide is prepared by compounding two insecticidal active components, and the insecticidal action mechanisms are different, so that the risk of drug resistance is reduced, and an effective direction can be provided for comprehensive treatment of drug-resistant pests. Secondly, the biopesticide provided by the invention is beneficial to reducing the dosage and frequency of the pesticide, so that the cost of preventing and treating the citrus red mites is reduced, and the environmental pressure is relieved.

The application provides a compound agent for preventing and treating termites based on Serratia marcescens, which comprises 7.75x10 8 ~2.63x10 12 CFU / mL of Serratia marcescens, and a pathogenic fungus or a biological pesticide or an insect growth regulator or a chemical agent; the pathogenic fungus is Beauvaria bassiana or Metarhizium anisopliae; the biological pesticide is ivermectin; the insect growth regulator is lufenuron or buprofezin or diflubenzuron; and the chemical agent is emamectin benzoate or indoxacarb. The compound agent has obvious synergistic effect on preventing and treating termites, reduces the use amount of single agent, is lower in cost, more efficient, stable and green.

The invention provides a microbial preparation, a preparation method and application in pest control, and relates to the technical field of microorganisms, the microbial preparation is mainly prepared from the following raw materials by mass: 40-50% of a complex microbial inoculant; 50%-60% of an additive; wherein the complex microbial inoculant is composed of a main microbial inoculant and an auxiliary microbial inoculant, and the main microbial inoculant is composed of 35% of verticillium lecanii, 35% of beauveria bassiana and 15% of insect viruses based on the mass percentage of the complex microbial inoculant; the auxiliary fungicide is prepared from 10% of metarhizium anisopliae and 5% of beauveria brongniartii. According to the microbial preparation, various bacteria and insect viruses are scientifically compounded, the synergistic interaction effect between the bacteria and between the bacteria and the viruses is exerted, and the good control effect on piercing-sucking mouthpart pests is achieved.

The application discloses a Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis, which is obtained by compounding a Beauveria bassiana 20251932 spore suspension and a Beauveria bassiana 2008658 spore suspension; the concentration of the Beauveria bassiana 20251932 spore suspension is preferably 1.928*10 6 spores / ml, and the concentration of the Beauveria bassiana 2008658 spore suspension is 8.711*10 6 spores / ml; the compounding volume ratio of the Beauveria bassiana 2008658 spore suspension and the Beauveria bassiana 20251932 spore suspension is preferably 1:10, 1:5, 1:2, 1:1 and 2:1 to achieve a synergistic effect; and the Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis has high pathogenicity when used for preventing and treating Bactrocera dorsalis adults after eclosion.

The invention belongs to the technical field of biological control, and particularly relates to application of protein coded by a prodenia litura gloverin gene in combination with beauveria bassiana in insect control. The technical problem to be solved by the invention is to provide a new method for biological control of agricultural pests. The technical scheme of the invention is the application of the protein coded by the prodenia litura gloverin gene in insect prevention and control. The amino acid sequence of the protein is shown as SEQ ID No.2. According to the method disclosed by the invention, the insects are effectively prevented and controlled by reducing the expression of the gloverin gene in the insects; meanwhile, the death rate of the insects can be further increased by combining the reduction of the expression of the gloverin gene in the insects with the combination of Beauveria bassiana.

The invention discloses a complex microbial inoculant for preventing and treating carya illinoensis perwing moths and application of the complex microbial inoculant, and belongs to the technical field of pest prevention and treatment. The complex microbial inoculant for preventing and treating the carya illinoensis pernicifuge is prepared from the following raw materials in parts by mass: 2 to 3 parts of beauveria bassiana bacterial liquid, 3 to 5 parts of bacillus thuringiensis bacterial liquid, 4 to 8 parts of brevibacillus laterosporus bacterial liquid, 5 to 6 parts of bacillus cereus bacterial liquid, 8 to 10 parts of sodium alginate, 3 to 5 parts of seaweed extract, 1 to 2 parts of liquorice root extract and 3 to 4 parts of soybean extract. The complex microbial inoculant disclosed by the invention has an excellent prevention and treatment effect on the perfond moth, and the problems that the existing chemical agent is easy to generate drug resistance and pesticide residue, destroys ecological balance, threatens food safety and human health and the like are solved.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The invention relates to the technical field of prevention and control of bactrocera cucurbitae, in particular to high-temperature-resistant beauveria bassiana WZS-5 and application thereof to prevention and control of bactrocera cucurbitae, and the preservation number of the high-temperature-resistant beauveria bassiana WZS-5 is GDMCCNO: 66358. The optimal growth temperature of the strain is 28-30 DEG C, and the strain still keeps certain growth and reproduction capability at the high temperature of 35 DEG C. Biological assay results show that under the conditions that the temperature is 30 DEG C and the relative humidity is 70%, the prevention and treatment effect of the strain on beauveria bassiana is remarkably better than that of common beauveria bassiana, and the accumulative death rate of bactrocera cucurbitae adults under the concentration of 1 * 10 < 9 > spores / mL reaches 89%. Results show that the strain has excellent high-temperature adaptability and strong pathogenic activity, can efficiently control bactrocera cucurbitae populations in tropical and subtropical high-temperature environments, and provides new microbial resources and technical approaches for green prevention and control of fruit and vegetable pests.

The invention discloses beauveria bassiana QXNYCJSZX2025-08 and application of the beauveria bassiana QXNYCJSZX2025-08 in a tobacco field, and belongs to the technical field of plant protection. The problems that in existing tobacco planting, pesticide residues exceed the standard, the pesticide resistance of pests is enhanced and the ecological environment is damaged due to the fact that chemical pesticides are relied on for preventing and controlling underground pests (such as grubs and cutworms) for a long time, and existing biocontrol bacteria (such as single growth-promoting bacteria or insecticidal bacteria) can only achieve the single function of growth promotion or insect killing are solved. The invention provides an environment-friendly alternative scheme which is characterized in that continuous biological control is realized through endophytic colonization of the beauveria bassiana QXNYCJSZX2025-08 strain, and triple functions of promoting tobacco plant growth (increasing yield and improving quality), enhancing disease resistance and preventing and controlling underground pests are integrated into a whole; efficient utilization of resources and field synergistic interaction are realized. Meanwhile, the invention further develops a matched field application scheme, and empirical support is provided for technical landing.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.

The invention relates to the technical field of microorganisms, in particular to beauveria bassiana QZ and application thereof. The collection number of the beauveria bassiana QZ is GDMCC No: 66669. The beauveria bassiana QZ provided by the invention has strong pathogenicity to coffee berry bark beetles, the strain is modified to express and deliver double-stranded RNA-dsDorsal, the pathogenicity is stronger, laboratory data shows that the fatality rate is almost 100% within 8 days, and the lethal ability is improved by 20% compared with that of a non-transgenic strain.

The application discloses a Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis, which is obtained by compounding a Beauveria bassiana 20251932 spore suspension and a Beauveria bassiana 2008658 spore suspension; the concentration of the Beauveria bassiana 20251932 spore suspension is preferably 1.928*10 6 spores / ml, and the concentration of the Beauveria bassiana 2008658 spore suspension is 8.711*10 6 spores / ml; the compounding volume ratio of the Beauveria bassiana 2008658 spore suspension and the Beauveria bassiana 20251932 spore suspension is preferably 1:10, 1:5, 1:2, 1:1 and 2:1 to achieve a synergistic effect; and the Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis has high pathogenicity when used for preventing and treating Bactrocera dorsalis adults after eclosion.

The invention relates to the technical field of fertilizer preparation, in particular to a fertilizer containing a natural insect repelling component and a preparation method thereof.The fertilizer is prepared from, by weight, 20-80 parts of an organic matrix, 0.5-10 parts of the natural insect repelling component, 3-35 parts of an inorganic nutrient component, 0.05-2.0 parts of a microbial agent, 0.05-1.0 part of beauveria bassiana and 0.1-6 parts of auxiliaries; the natural parasite expelling components and the beauveria bassiana are compounded to form a synergistic parasite expelling system, and the parasite expelling system, the organic matrix, the inorganic nutrient components, the microbial agent and the auxiliaries have a synergistic effect, so that integration of parasite expelling, nutrient supply and soil improvement is realized.

The application provides a Beauveria bassiana strain Bb-YY for preventing and controlling various diseases and pests, a derivative product and application thereof, and belongs to the technical field of functional microorganisms. Beauveria bassiana The strain Bb-YY is screened from the rhizosphere soil of a peanut healthy plant planted in a serious continuous cropping obstacle plot, and is identified as Beauveria bassiana (Bb) by molecular identification, has high efficient prevention and control effects on white grubs, peanut fruit rot pathogens and peanut root rot pathogens, and has certain inhibition effects on Bacterospora coriacea causing tobacco black foot disease and Pyricularia oryzae causing rice blast. It can be seen that the Bb-YY strain can also play a prevention and control effect on tobacco, rice and other crops in addition to peanut planting, and can effectively reduce the use of chemical pesticides, and provides a strong support for green prevention and control of peanut diseases and pests.

The invention belongs to the technical field of biological control of agricultural pests, and discloses a method for controlling diaphorina citri through beauveria bassiana based on efficient targeting spore powder and application of the method, and the method comprises the steps that the efficient targeting spore powder is prepared, the collection number of a beauveria bassiana strain is CGMCC No.40957, the spore content is larger than or equal to 10 billion / g, the viable spore rate is larger than or equal to 90%, the beauveria bassiana strain is mixed with a functional auxiliary agent according to the weight ratio of 100: (1-5), and the efficient targeting spore powder is prepared; the functional additive comprises a biochemical permeation promoter, an environment shielding and targeting adhesive and an insect behavior synergist; applying the spore powder to the diaphorina inhabitation area of the citrus plant in a targeted manner by using an electrostatic powder spraying device; and arranging a light insect trapping plate to induce the phylloxera to be in contact with the spore powder. According to the method, the pathogenicity and stress resistance of the beauveria bassiana conidial powder are improved by adding the permeation promoter and the protective agent, and the electrostatic powder spraying and light inducing technologies are combined to realize precise targeting and efficient prevention and treatment of diaphorina citri and reduce the use of chemical pesticides.

The invention relates to the technical field of agricultural microorganism application, in particular to a beauveria bassiana Bb455 strain with efficient nitrogen fixation capacity and application of the strain in promotion of corn growth, and the beauveria bassiana Bb455 strain is suitable for green planting of grain crops such as corn and the like, can reduce application of chemical nitrogen fertilizer and can promote sustainable development of agricultural ecology.

The invention belongs to the technical field of biocontrol microorganism genetic engineering, and particularly relates to an sfgA gene for regulating and controlling growth and development of beauveria bassiana and application of the sfgA gene. The invention discloses an sfgA gene for regulating and controlling growth and development of beauveria bassiana, the nucleotide sequence of the sfgA gene is as shown in SEQ ID NO.1, and the amino acid sequence of encoded protein of the sfgA gene is as shown in SEQ ID NO.2. By systematically researching the effect of the development signal protein sfgA in the asexual spore production process of the beauveria bassiana and the regulation and control mechanism of the development signal protein sfgA, the growth and development of the beauveria bassiana can be regulated and controlled. A theoretical basis is laid for researching a filamentous fungus asexual sporulation molecular mechanism, and a new scientific basis and gene resources are provided for mining and genetic improvement of biocontrol potentials of biocontrol fungi.

The invention discloses a beauveria bassiana insecticidal composition with high pathogenicity to bactrocera dorsalis. The beauveria bassiana insecticidal composition is prepared by compounding a beauveria bassiana 20251932 spore suspension and a beauveria bassiana 2008658 spore suspension, preferably, the concentration of the spore suspension of the beauveria bassiana 20251932 is 1.928 * 10 < 6 > spores / ml, and the concentration of the spore suspension of the beauveria bassiana 2008658 is 8.711 * 10 < 6 > spores / ml; the synergistic effect is achieved when the compounding volume ratio of the beauveria bassiana 2008658 spore suspension to the beauveria bassiana 20251932 spore suspension is preferably 1: 10, 1: 5, 1: 2, 1: 1 and 2: 1; the beauveria bassiana insecticidal composition with high pathogenicity to bactrocera dorsalis has high pathogenicity when being used for preventing and treating adult bactrocera dorsalis after eclosion.

The present invention provides stable, non-aqueous biopesticides. The present invention relates to biopestic formulations of entomopathogenic fungi or their spores. In particular, the present invention discloses oil dispersions containing entomopathogenic fungi or their spores, especially Beauveria bassiana. The present invention also relates to methods for preparing the formulations and their use.

The invention discloses a strain of Beauveria bassiana YJ-YC1 and an application of the Beauveria bassiana YJ-YC1 in prevention and treatment of lepidoptera pests, and particularly discloses a strain of Beauveria bassiana YJ-YC1. The beauveria bassiana YJ-YC1 provided by the invention is obtained by separating from the female ozostoma canarium bombyx batryticatus collected from a mangrove forest in Guangdong province, the preservation number of the beauveria bassiana YJ-YC1 is GDMCC NO: 66725, the beauveria bassiana YJ-YC1 has very strong pathogenicity to larvae and pupae of a plurality of outbreak lepidoptera pests (female ozostoma canarium, teak camel moth and heortia vitessoides), and the beauveria bassiana YJ-YC1 has the advantages of high bombyx batryticatus rate, no toxic or side effect and no toxic or side effect. The strain is a biological control bacterium with potential value in the control of lepidoptera pests, and has good application prospects.

The present application relates to the field of microorganisms, and particularly relates to a preparation method and application of a Beauveria bassiana BC-01 mycelium polysaccharide, wherein the Beauveria bassiana BC-01 has been preserved in the China General Microbiological Culture Collection Center on January 18, 2024, and the preservation number is CGMCC No. 41044. The strain produces mycelium polysaccharide with an IC 50 value of 0.5302 mg / mL for inhibiting tyrosinase; and a polysaccharide concentration of 1.5 mg / mL shows good whitening effect. The polysaccharide promotes the expression of cytokines IL-6 and TNF-alpha, and shows immune activity. The polysaccharide has the effects of whitening, antioxidation, and immune activation, and can promote the proliferation of Staphylococcus epidermidis and inhibit the proliferation of Propionibacterium acnes, and can be used as a new whitening and skin microecological material for developing cosmetics. The preparation method of the Beauveria bassiana mycelium polysaccharide is simple and easy to operate, the extraction process does not use organic reagents such as ethanol and methanol, and the production is safe and low in price.

The invention discloses a high-virulence beauveria bassiana Zeb-01 strain and application of the high-virulence beauveria bassiana Zeb-01 strain to control of palm pests, the high-virulence beauveria bassiana Zeb-01 strain is beauveria bassiana, and the preservation name of the high-virulence beauveria bassiana Zeb-01 strain is Beauveria bassiana; the strain is preserved in the China General Microbiological Culture Collection Center, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is November 15, 2025; the preservation number of the strain is CGMCC (China General Microbiological Culture Collection The separated beauveria bassiana Zeb-01 strain has extremely high pathogenicity to rhynchophorus ferrugineus and rhinoceros rhinoceros, the lethal effect of the strain is remarkably higher than that of commercially available beauveria bassiana preparations, the strain has more outstanding advantages under the low-concentration condition, the lethal time of the strain to the two pests is remarkably shorter than that of commercially available strains, the pest hazard period is effectively shortened, and the environment pollution is reduced. And the insecticidal composition has the characteristics of high toxicity, high insecticidal rate and wide range of applicable pests.

The present application relates to the field of steroid drugs and biotechnology, and relates to a novel steroid hydroxylase CYP68BE1 from Beauveria bassiana, a coding gene thereof and application. Specifically, the present application provides a novel steroid hydroxylase CYP68BE1 from Beauveria bassiana, a nucleic acid molecule coding the enzyme, a vector containing the nucleic acid molecule, and a recombinant bacterium containing the vector or having the nucleic acid molecule integrated therein. Through recombination of yeast bacteria or microsomal transformation of steroids, it is found that the CYP68BE1 enzyme can catalyze the hydroxylation reaction of C11α and / or C6β and / or C1β and / or C1α of steroid compounds. Among them, the transformation products include various steroid drug intermediates and / or steroid active compounds, and the present application has great application potential and economic value.

The application provides a method for preparing Beauveria bassiana protoplast by using lyticase. The method comprises the following steps: obtaining Beauveria bassiana mycelium and washing the mycelium with a NaCl solution; mixing the washed mycelium with an enzyme solution according to a ratio of 1g:50mL and oscillating the enzyme solution for enzymolysis; after the enzymolysis is completed, post-treating the enzyme solution to obtain the Beauveria bassiana protoplast; and the concentration of the lyticase in the enzyme solution is 5-20g / L per gram. Under the optimal enzymolysis condition, the yield of the Beauveria bassiana protoplast reaches 2.56*10 7 6 / mL, which is about 2.56 times higher than that of the prior art.

The invention relates to application of silkworm excrement as a raw material of a culture medium for producing beauveria bassiana, and a method for producing the beauveria bassiana by utilizing silkworm excrement waste, belonging to the field of fermentation. The invention also provides a beauveria bassiana solid culture medium which is characterized in that the raw materials of the culture medium contain silkworm excrement. The invention provides a fermentation culture technology using waste silkworm excrement, and aims to improve the production efficiency of beauveria bassiana and provide a new resource utilization mode for the production of bombyx batryticatus. The technology not only can effectively reduce the environmental pollution caused by wastes, but also can improve the production efficiency, save the raw material cost and improve the product quality.

This invention relates to the field of spore collection technology, specifically to a collection device for Beauveria bassiana spores from the medicinal silkworm. It includes a frame, a horizontal vibrating collection frame slidably connected to the frame, and a horizontal drive mechanism on the frame that drives the horizontal vibrating collection frame to vibrate alternately with a first, second, and third amplitude. A vertical vibrating collection frame is slidably connected to the horizontal vibrating collection frame, and a vertical drive mechanism between the two drives the vertical vibrating collection frame to vibrate alternately with a fourth, fifth, and sixth amplitude. A front collection cylinder and a rear collection cylinder are both fixedly connected to the horizontal vibrating collection frame. A collection shaft tube coaxially arranged with the front collection cylinder and a conveying shaft coaxially arranged with the rear collection cylinder are rotatably connected to the vertical vibrating collection frame. The beneficial effect of this invention is that it organically integrates mechanical peeling, airflow purging, and negative pressure suction to form an integrated continuous collection process of peeling, purging, and suction.