95 results about "Beauveria amorpha" patented technology

The invention relates to the technical field of microorganisms, in particular to a method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn and application thereof.The method for promoting corn growth to improve the resistance of plants to northern leaf blight of corn comprises the following specific steps that S1, a test field is subjected to scribing treatment and divided into four test fields, each test field is divided into four ridges, the ridge width is 0.61 m, and the ridge width is 0.6 m; the ridge length is 15 m, the aisle between each cell is 2 m, and the front and rear protection rows of the test area are 10 m respectively; s2, a first area is a blank control area for normal planting of corn plants, a second area is a second area for drip irrigation of beauveria bassiana planting (Bb) for the corn plants, a third area is a third area for northern leaf blight inoculation planting (Et) for the corn plants, and a fourth area is a fourth area for drip irrigation of beauveria bassiana planting (Bb + Et) after northern leaf blight inoculation for the corn plants. Beauveria bassiana applied in a drip irrigation mode has good effects on corn growth and resistance.

The present invention relates to microencapsulated preparations comprising biopesticide, bio-fungicide, and plant growth promoting agents and uses thereof, as well as to methods for controlling pests and for producing said preparations for managing insect pests. In particular, the invention relates to microencapsulated preparations comprising at least one insect-pathogenic (entomopathogenic) fungus selected from Metarhizium spp. and Beauveria bassiana, and at least one pathogenic Bacillus spp. bacterial strain, preparations and particles comprising these, their use on plant material, methods for controlling pests therewith, and processes for their production.

The invention provides a compound microbial agent capable of resisting diseases and insect pests and a preparation method thereof, and the compound microbial agent consists of the following substances in parts by mass: 50-100 parts of bacillus subtilis, 40-80 parts of beauveria bassiana, 35-75 parts of trichoderma harzianum, 20-50 parts of modified straw powder, 10-15 parts of a humic acid-diatomite complex, 10-15 parts of trehalose, 5-10 parts of chitin powder and 5-10 parts of shellac. According to the invention, the modified straw powder and the humic acid-diatomite complex are adopted as a core carrier, so that the defects of poor adsorbability and nutrition deficiency of the traditional diatomite or synthetic carrier are overcome, and a high-porosity and nutrient-rich living environment is provided for bacillus subtilis, beauveria bassiana and trichoderma harzianum; compared with the prior art, the method provided by the invention has the advantages that the cost is low, the field planting rate and the field survival rate of viable bacteria in the microbial inoculum are remarkably improved, meanwhile, controllable coating is performed by utilizing an ethanol solution of shellac, a refractory synthetic polymer coating material is replaced, thalli are protected from environmental stress, and meanwhile, the risk of lasting residual pollution of a chemical coating material in soil is avoided.

The invention provides a genetically engineered bacterium for producing R-2-(4-hydroxyphenoxy) propionic acid at high yield as well as a construction method and application of the genetically engineered bacterium. Based on metabolic engineering and efflux transport engineering strategies, transmembrane transport protein coding genes selected from an NPF protein family and an SWEET protein family are constructed into beauveria bassiana capable of producing R-2-(4-hydroxyphenoxy) propionic acid, and genetic engineering bacteria capable of stably and highly producing R-2-(4-hydroxyphenoxy) propionic acid are constructed. Compared with a control strain, an experimental group has the advantages that the yield of R-2-(4-hydroxyphenoxy) propionic acid subjected to liquid fermentation is remarkably increased, and the fermentation period is shortened. The method for constructing the high-yield R-2-(4-hydroxyphenoxy) propionic acid gene engineering strain by transforming the beauveria bassiana on the basis of efflux transport engineering provides an important target for efficiently producing the R-2-(4-hydroxyphenoxy) propionic acid by transforming the beauveria bassiana, and has an industrial application prospect.

The invention discloses a bacterial strain suitable for large-scale production of high-quality stiff silkworm and application of the bacterial strain. The strain is a beauveria bassiana KW2 strain, and is preserved in Guangdong Microbial Culture Collection Center on November 19, 2024, and the preservation number is GDMCC NO: 65502. When the KW2 strain is used for producing stiff silkworms, the stiff silkworms are pink in color and then gradually fade, so that efficient sorting of the stiff silkworms is facilitated, the quality of the stiff silkworms can be better controlled, the labor cost can be better reduced, and the method is very suitable for large-scale production of the stiff silkworms. Meanwhile, hyphae on the surface of the stiff silkworm are oily and thick, less spore powder is produced, and production environment management and personnel health are facilitated. The KW2 strain has a silkworm death rate of 100%, the bombyx batryticatus is cylindrical and high in quality, and various indexes such as moisture and the like meet standards. Moreover, the content of beauveria bassiana and ammonium oxalate is relatively high, and the medicinal value is high. The KW2 strain has a very good application prospect in improving the large-scale safe production efficiency of high-quality stiff silkworms.

The invention mainly provides Beauveria bassiana ZABb200102 and application of the Beauveria bassiana ZABb200102, the Beauveria bassiana ZABb200102 is preserved in China General Microbiological Culture Collection Center on October 28, 2024, and the preservation number of the Beauveria bassiana ZABb200102 is CGMCC (China General Microbiological Culture Collection Center) No. 41557. The strain is separated from sick lepidoptera cacalidae larva bodies for the first time, and it is found through toxicity determination that the strain has high pathogenicity to tomato leaf miner larvae. In addition, the strain can also be colonized in tomatoes, so that the growth of the tomatoes is promoted, and the resistance of the tomatoes to pests is indirectly improved. Therefore, the strain can be widely applied to development of tomato bacterial fertilizer and green prevention and control of tomato leaf miner which is an important tomato pest.

The present invention relates to a graphene oxide - Beauveria bassiana complex and its application in pest control and improving crop growth. The invention provides a graphene oxide - Beauveria bassiana complex, and optimizes the ratio of graphene oxide to Beauveria bassiana. Graphene oxide at a concentration of 20 μg / ml - 100 μg / ml has a UV - protecting effect on Beauveria bassiana spores. The experimental results show that the graphene oxide - Beauveria bassiana provided in this application can significantly improve the control effect of Beauveria bassiana on pests, and the seed soaking treatment can also significantly improve the growth indexes of crops such as plant height, leaf length, leaf width and leaf area, having unexpected technical effects and very good agricultural application value.

The present invention provides a dsRNA targeting the BcPfy1 gene, a Beauveria bassiana engineered strain Bb-dsPfy1, and their applications. The Beauveria bassiana engineered strain Bb-dsPfy1 uses the Beauveria bassiana QSE-F1 strain as the chassis strain, and this strain can colonize the tomato leaves. The dsRNA is a double-stranded RNA fragment designed with the Botrytis cinerea BcPfy1 gene as the target, which has the functions of interfering with the expression of the BcPfy1 gene and inhibiting the infection of Botrytis cinerea. This fragment is obtained by in vitro synthesis or synthesized in the QSE-F1 strain through two target gene sequences with the same sequence and opposite directions. The engineered strain Bb-dsPfy1 is obtained by using the ATMT technique to transfer a vector containing the target gene sequence into the Beauveria bassiana QSE-F1 strain, and it is an engineered strain capable of synthesizing dsRNA targeting BcPfy1. It is demonstrated by experiments in the present invention that the engineered strain can effectively interfere with the expression of the target gene BcPfy1 and has a significant control effect on the occurrence of tomato gray mold. This engineered strain can be used to prepare biological agents for controlling gray mold and has good market application prospects.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The present disclosure relates to a Beauveria bassiana KNU-101 strain. The Beauveria bassiana KNU-101 strain is entomopathogenic, exhibits an excellent insecticidal effect on controlling thrips, which are soil pests that harm crops, and also has excellent sporulation ability. Thus, the Beauveria bassiana KNU-101 strain may be effectively used as an environmentally friendly means for controlling pests.

The invention provides a secretory protein with insecticidal activity and an application of the secretory protein in pest control, an entomopathogenic fungus beauveria bassiana is used as a research material, and the secretory protein BbTsp1 is identified by using a protein spectrum technology. The protein is prepared and purified by using a prokaryotic expression system. A host blood cavity injection experiment proves that the protein has the activity of killing a host. Through a gene recombination technology, the BbTsp1 gene is introduced into beauveria bassiana, and an overexpression strain of the gene is constructed. Bioassay shows that the insecticidal activity of the strain is remarkably improved. The construction of a recombinant strain of the toxicity related gene identified by the invention improves the pest control efficiency of insecticidal fungi.

The invention discloses a duplex PCR primer group for detecting nosema bombycis and beauveria bassiana. The duplex PCR primer group comprises an upstream primer and a downstream primer which are used for detecting nosema bombycis, and an upstream primer and a downstream primer which are used for detecting beauveria bassiana, wherein the nucleotide sequences of the upstream primer and the downstream primer for detecting the nosema bombycis are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; the nucleotide sequences of the upstream primer and the downstream primer for detecting the beauveria bassiana are respectively shown as SEQ ID No. 3 and SEQ ID No. 4. The invention further provides application, a kit and a detection method. According to the present invention, the requirements of simultaneous diagnosis and monitoring of the nosema bombycis and the beauveria bassiana can be met, and characteristics of strong specificity, high sensitivity, wide detection range, high detection efficiency and rapid and simple operation are provided.

The present application belongs to the technical field of preparation of white-backed caterpillar. More particularly, it relates to a Beauveria bassiana agent for greatly improving the time concentration of death of domestic silkworm and a high-quality white-backed caterpillar production method. The present application provides a Beauveria bassiana agent containing a strain of Beauveria bassiana and a fermentation product of another strain of Beauveria bassiana without bacteria bodies. The use of the agent in the present application to infect domestic silkworm can shorten the time of complete death of domestic silkworm, and concentrate the death time of domestic silkworm at the 6th day after inoculation, with a time span of only 24 hours, which helps to improve the production efficiency of white-backed caterpillar, effectively reduces the labor of feeding and picking silkworms during the death stage, and reduces the production cost. In addition, the method for producing white-backed caterpillar in the present application can achieve good infection effect by spraying the agent once, reducing the process of white-backed caterpillar production. And the white-backed caterpillar produced by the method in the present application is uniform in size, the consistency of product quality parameters is good, and the quality is stable, which helps to improve the clinical application effect.

The invention belongs to the technical field of pesticides, and particularly relates to a biopesticide for preventing and treating citrus red mites, the active component of the biopesticide is formed by compounding beauveria bassiana and cyflumetofen, or is formed by compounding beauveria bassiana and guadipyr; the number of spores in the beauveria bassiana is 5 billion spores per gram. The compounded combination of the biopesticide has a synergistic effect on the citrus red mites within a certain mass ratio range, especially when the compounding mass ratio of the beauveria bassiana to the guadipyr is 1: 30 and 1: 20, the co-toxicity coefficient reaches 418.133 and 317.078, the synergistic effect is remarkable, and the prevention and treatment effect on the citrus red mites can be improved. Besides, the biopesticide is prepared by compounding two insecticidal active components, and the insecticidal action mechanisms are different, so that the risk of drug resistance is reduced, and an effective direction can be provided for comprehensive treatment of drug-resistant pests. Secondly, the biopesticide provided by the invention is beneficial to reducing the dosage and frequency of the pesticide, so that the cost of preventing and treating the citrus red mites is reduced, and the environmental pressure is relieved.

The invention discloses a method for cultivating and producing white muscardine silkworms by using 5-year-old silkworm larvae as a culture medium. The method specifically comprises the following steps: (1) quality improvement and optimization of a mother strain of beauveria bassiana; (2) carrying out stress stimulation culture on the beauveria bassiana solid-liquid mixed leavening agent; (3) carrying out inoculation treatment on a culture medium of 5-year-old silkworm larvae; and (4) white muscardine silkworm spawn running management. According to the method, the silkworm larvae of 5 years old are used as the culture medium to breed and produce the silkworm larva, a new way for preparing the medicinal silkworm larva is provided, and the silkworm larvae of 5 years old can be utilized and converted into the silkworm larva to accumulate nutrition and bioactive components to the greatest extent; the method is not limited by weak infection lethal ability of the beauveria bassiana strain and strong resistance of the hybridized combined silkworm variety, the beauveria bassiana with high bioactive component attribute and the large hybridized combined silkworm variety with rich nutrition and mass can be fully compatible, the quality of the silkworm larva medicinal material is ensured, meanwhile, the yield is remarkably improved, and the method is stable, efficient and wide in development prospect.

The application provides a compound agent for preventing and treating termites based on Serratia marcescens, which comprises 7.75x10 8 ~2.63x10 12 CFU / mL of Serratia marcescens, and a pathogenic fungus or a biological pesticide or an insect growth regulator or a chemical agent; the pathogenic fungus is Beauvaria bassiana or Metarhizium anisopliae; the biological pesticide is ivermectin; the insect growth regulator is lufenuron or buprofezin or diflubenzuron; and the chemical agent is emamectin benzoate or indoxacarb. The compound agent has obvious synergistic effect on preventing and treating termites, reduces the use amount of single agent, is lower in cost, more efficient, stable and green.

The invention discloses a composite agent for preventing and treating rice blast, and belongs to the technical field of crop sterilization agents. The agent comprises a fungicide, an antiviral agent, a disease and pest resistant agent and a compound microbial agent according to a mass ratio. Wherein the fungicide is mainly formed by mixing iprodione, spinosad and cyprodinil, the antiviral agent is formed by mixing glucosamine, ribonuclease and beta-carotene, the disease and pest resistant agent is formed by mixing glyphosate and glufosinate-ammonium, and the compound microbial agent is formed by compounding beauveria bassiana and beauveria bassiana. Through reasonable matching of the components, the synergistic effect is achieved, the resistance of the components is reduced, and the pesticide effect is improved. In addition, the composite agent not only can effectively control the occurrence of rice blast, but also can improve the resistance of plants to the rice blast by enhancing the stress resistance and immune function of the plants, thereby achieving the purpose of prevention and treatment.

The invention provides dsRNA (double-stranded ribonucleic acid) of a targeted BcPfy1 gene, a beauveria bassiana engineering strain Bb-dsPfy1 and application of the beauveria bassiana engineering strain Bb-dsPfy1. According to the Beauveria bassiana engineering strain Bb-dsPfy1, a Beauveria bassiana QSE-F1 strain is used as a chassis bacterium, and the strain can be colonized at tomato leaves. The dsRNA is a double-stranded RNA fragment which is designed by taking a botrytis cinerea BcPfy1 gene as a target and has the functions of interfering BcPfy1 gene expression and inhibiting botrytis cinerea infection, and the fragment is obtained through in-vitro synthesis or is synthesized in a QSE-F1 strain through two target gene sequences which are the same in sequence and opposite in direction. The engineering strain Bb-dsPfy1 is a dsRNA engineering strain capable of synthesizing targeted BcPfy1, which is obtained by transferring a carrier containing a target gene sequence into a beauveria bassiana QSE-F1 strain by utilizing an ATMT technology. Experiments prove that the engineering strain can effectively interfere the expression of a target gene BcPfy1, has a remarkable prevention and treatment effect on tomato gray mold, can be used for preparing biological agents for preventing and treating gray mold, and has a good market application prospect.

The invention provides a microbial preparation, a preparation method and application in pest control, and relates to the technical field of microorganisms, the microbial preparation is mainly prepared from the following raw materials by mass: 40-50% of a complex microbial inoculant; 50%-60% of an additive; wherein the complex microbial inoculant is composed of a main microbial inoculant and an auxiliary microbial inoculant, and the main microbial inoculant is composed of 35% of verticillium lecanii, 35% of beauveria bassiana and 15% of insect viruses based on the mass percentage of the complex microbial inoculant; the auxiliary fungicide is prepared from 10% of metarhizium anisopliae and 5% of beauveria brongniartii. According to the microbial preparation, various bacteria and insect viruses are scientifically compounded, the synergistic interaction effect between the bacteria and between the bacteria and the viruses is exerted, and the good control effect on piercing-sucking mouthpart pests is achieved.

The application discloses a Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis, which is obtained by compounding a Beauveria bassiana 20251932 spore suspension and a Beauveria bassiana 2008658 spore suspension; the concentration of the Beauveria bassiana 20251932 spore suspension is preferably 1.928*10 6 spores / ml, and the concentration of the Beauveria bassiana 2008658 spore suspension is 8.711*10 6 spores / ml; the compounding volume ratio of the Beauveria bassiana 2008658 spore suspension and the Beauveria bassiana 20251932 spore suspension is preferably 1:10, 1:5, 1:2, 1:1 and 2:1 to achieve a synergistic effect; and the Beauveria bassiana insecticidal composition with high pathogenicity to Bactrocera dorsalis has high pathogenicity when used for preventing and treating Bactrocera dorsalis adults after eclosion.

The invention belongs to the technical field of biological control, and particularly relates to application of protein coded by a prodenia litura gloverin gene in combination with beauveria bassiana in insect control. The technical problem to be solved by the invention is to provide a new method for biological control of agricultural pests. The technical scheme of the invention is the application of the protein coded by the prodenia litura gloverin gene in insect prevention and control. The amino acid sequence of the protein is shown as SEQ ID No.2. According to the method disclosed by the invention, the insects are effectively prevented and controlled by reducing the expression of the gloverin gene in the insects; meanwhile, the death rate of the insects can be further increased by combining the reduction of the expression of the gloverin gene in the insects with the combination of Beauveria bassiana.

The invention belongs to the technical field of biological control, and particularly relates to beauveria bassiana TYW001 and application thereof. The invention provides beauveria bassiana TYW001, the Latin name of the beauveria bassiana TYW001 is Beauveria bassiana, the beauveria bassiana TYW001 is preserved in China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 30, 2025, and the preservation number is CGMCC NO.42149. The strain not only can resist high temperature and drought, but also can resist high temperature and drought, but also can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and drought, can resist high temperature and can resist high temperature the soybean megalurothrips can be effectively prevented and treated; moreover, the beauveria bassiana TYW001 can enhance the predation effect of the orius minutus on the megalurothrips megalurothrips, and a reliable green prevention and control technology is provided for prevention and control of the megalurothrips megalurothrips.

The invention discloses a complex microbial inoculant for preventing and treating carya illinoensis perwing moths and application of the complex microbial inoculant, and belongs to the technical field of pest prevention and treatment. The complex microbial inoculant for preventing and treating the carya illinoensis pernicifuge is prepared from the following raw materials in parts by mass: 2 to 3 parts of beauveria bassiana bacterial liquid, 3 to 5 parts of bacillus thuringiensis bacterial liquid, 4 to 8 parts of brevibacillus laterosporus bacterial liquid, 5 to 6 parts of bacillus cereus bacterial liquid, 8 to 10 parts of sodium alginate, 3 to 5 parts of seaweed extract, 1 to 2 parts of liquorice root extract and 3 to 4 parts of soybean extract. The complex microbial inoculant disclosed by the invention has an excellent prevention and treatment effect on the perfond moth, and the problems that the existing chemical agent is easy to generate drug resistance and pesticide residue, destroys ecological balance, threatens food safety and human health and the like are solved.

The invention relates to the field of insect prevention and control, in particular to a high-stress-resistance beauveria bassiana strain CIPPBb075 with high pathogenicity to grassland caterpillars and application of the high-stress-resistance beauveria bassiana strain CIPPBb075. According to the invention, diseased insects parasitized by beauveria bassiana are collected from a field, and the strain is separated and purified, so that the CIPPBb075 strain has relatively high toxicity to grassland caterpillar; the germination rate is increased along with temperature rise, and the spore germination rate is obviously higher than that of other strains; the ultraviolet resistance at 25 DEG C is high; the extracellular protease activity is high; the activity of extracellular chitinase is high. Therefore, the CIPPBb075 strain has relatively strong stress resistance, and can be used as an effective microbial agent for preventing and treating the larvae of the grassland caterpillar compared with other strains.

The invention relates to the technical field of prevention and control of bactrocera cucurbitae, in particular to high-temperature-resistant beauveria bassiana WZS-5 and application thereof to prevention and control of bactrocera cucurbitae, and the preservation number of the high-temperature-resistant beauveria bassiana WZS-5 is GDMCCNO: 66358. The optimal growth temperature of the strain is 28-30 DEG C, and the strain still keeps certain growth and reproduction capability at the high temperature of 35 DEG C. Biological assay results show that under the conditions that the temperature is 30 DEG C and the relative humidity is 70%, the prevention and treatment effect of the strain on beauveria bassiana is remarkably better than that of common beauveria bassiana, and the accumulative death rate of bactrocera cucurbitae adults under the concentration of 1 * 10 < 9 > spores / mL reaches 89%. Results show that the strain has excellent high-temperature adaptability and strong pathogenic activity, can efficiently control bactrocera cucurbitae populations in tropical and subtropical high-temperature environments, and provides new microbial resources and technical approaches for green prevention and control of fruit and vegetable pests.

The invention provides recombinant beauveria bassiana for catalyzing a high-concentration substrate as well as a construction method and application of the recombinant beauveria bassiana. Based on a metabolic engineering and efflux transport engineering strategy, cytochrome P450 enzyme or a transmembrane transport protein coding gene selected from an NPF protein family and an SWEET protein family is constructed into beauveria bassiana for producing R-2-(4-hydroxyphenoxy) propionic acid; the recombinant beauveria bassiana capable of stably catalyzing the conversion of the high-concentration R-2-phenoxypropionic acid into the R-2-(4-hydroxyphenoxy) propionic acid is constructed. Compared with a control strain, an experimental group has the advantages that the tolerance to the concentration of R-2-phenoxypropionic acid is obviously improved, and the fermentation period is shortened. The construction method provided by the invention provides an important target for transforming high-concentration R-2-phenoxypropionic acid into R-2-(4-hydroxyphenoxy) propionic acid under the catalysis of the transformed beauveria bassiana, and has an industrial application prospect.

The invention discloses beauveria bassiana QXNYCJSZX2025-08 and application of the beauveria bassiana QXNYCJSZX2025-08 in a tobacco field, and belongs to the technical field of plant protection. The problems that in existing tobacco planting, pesticide residues exceed the standard, the pesticide resistance of pests is enhanced and the ecological environment is damaged due to the fact that chemical pesticides are relied on for preventing and controlling underground pests (such as grubs and cutworms) for a long time, and existing biocontrol bacteria (such as single growth-promoting bacteria or insecticidal bacteria) can only achieve the single function of growth promotion or insect killing are solved. The invention provides an environment-friendly alternative scheme which is characterized in that continuous biological control is realized through endophytic colonization of the beauveria bassiana QXNYCJSZX2025-08 strain, and triple functions of promoting tobacco plant growth (increasing yield and improving quality), enhancing disease resistance and preventing and controlling underground pests are integrated into a whole; efficient utilization of resources and field synergistic interaction are realized. Meanwhile, the invention further develops a matched field application scheme, and empirical support is provided for technical landing.

The invention discloses a Beauveria bassiana Bbzy2407 strain and application thereof, the strain is Beauveria bassiana, and the strain is classified and named as Beauveria bassiana; the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is December 02, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The beauveria bassiana Bbzy2407 provided by the invention is a biocontrol fungus capable of being used for preventing and controlling major pest Holotrichia crassipes larvae of agriculture and forestry plants, has the characteristics of strong pathogenicity to the Holotrichia crassipes larvae, safety to the environment and difficulty in causing drug resistance of pests, and can be used for biologically preventing and controlling the Holotrichia crassipes larvae.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The present application relates to the field of insect control, in particular to a strong stress-resistant Beauveria bassiana strain CIPPBb075 with high pathogenicity to lema frisoni and its application. The present application is collected from the field by the diseased insects parasitized by the Beauveria bassiana, and the strain is separated and purified, the CIPPBb075 strain has high virulence to lema frisoni; the germination rate increases with the increase of temperature, and the spore germination rate is significantly higher than that of other strains; the ultraviolet resistance is high at 25 DEG C; the extracellular protease activity is high; the extracellular chitinase activity is high. Therefore, the CIPPBb075 strain has strong stress resistance and other strains, and can be used as an effective microbial source drug for controlling lema frisoni larvae.