A tetracycline-resistant Escherichia coli and its application

A technology of Escherichia coli and tetracycline, applied in the biological field, can solve the problems of easy contamination of strains, long time-consuming, cumbersome operation, etc., and achieve good pathogenicity, short time-consuming, and clear-cut effects

Inactive Publication Date: 2020-11-13
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the present application provides a tetracycline-resistant E. Escherichia coli and its application

Method used

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  • A tetracycline-resistant Escherichia coli and its application
  • A tetracycline-resistant Escherichia coli and its application
  • A tetracycline-resistant Escherichia coli and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The isolation and identification of embodiment 1 bacteria

[0034] 1. Isolation of bacteria

[0035] (1) Isolation, culture and morphological observation of bacteria

[0036] Take the dead duck liver tissue and aseptically inoculate it on the MacConkey agar plate medium, culture it at a constant temperature of 37°C for 18-24 hours to observe the growth of the bacteria; pick a single colony that turns pink on the medium for Gram staining microscopy, Then pick the same single colony and inoculate it on the MacConkey agar plate medium for further separation and purification, and re-inoculate the isolated and purified bacteria on the agar slant, coded as A, and save it for future use.

[0037] (2), strain A tetracycline susceptibility test

[0038] Strain A was evenly spread and inoculated on the nutrient agar medium, and the tetracycline susceptibility test was carried out according to the K-B disk diffusion method (Shasha, Song Zhenhui. Animal Microbiology Experiment Tu...

Embodiment 2B3

[0060] Example 2B 3 Strain animal regression test

[0061] Will B 3 The strain was inoculated in the nutrient broth medium, shaken and cultured at 37°C for 12 hours, and the bacterial liquid was subjected to McFarland turbidity to make the bacterial liquid content 1×10 8 .cfu / mL (0.5 McFarland concentration). 10 healthy ducklings in the test were divided into 2 groups, respectively, the test group and the control group, 5 in each group; the test group ducklings were intramuscularly injected with 1mL / of the inoculated bacteria solution, and the control group of ducklings were intramuscularly injected with normal saline, 1mL / , and observed 1 week, the incidence and mortality of ducklings were recorded.

[0062] Sensitive bacteria group A and resistant bacteria B 3 The test ducks in the group all showed depression, loss of appetite, unsteady standing, and thin stool after 24 hours of inoculation, died after 36 hours, and all died after 96 hours. Necropsy of dead ducklings s...

Embodiment 3

[0066] In vitro inhibitory effect of embodiment 3 Chinese medicine on tetracycline-resistant B3 bacteria

[0067] (1) Preparation of the tested Chinese medicine solution: take 4 g of five Chinese medicine extracts (each g extract is equivalent to 10 g of the original medicinal material) of Scutellaria baicalensis, rhubarb, Folium Folium, Radix Isatidis and Forsythia, and put them into different beakers respectively. , add 20ml of distilled water, stir to dissolve, filter, and sterilize the filtrate in a vial by steam under reduced pressure (115°C; 20min) for later use. At this time, the concentration of the medicinal solution was 2 g / ml.

[0068] (2) Bacterial solution preparation: inoculate the bacterial strain B3 preserved in glycerol at -20°C on ordinary agar medium, incubate at 37°C for 24 hours, pick typical colonies and inoculate them in ordinary broth medium, and incubate at 37°C for 18-24 hours ,spare.

[0069] (3) Antibacterial test of traditional Chinese medicine b...

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Abstract

The invention discloses tetracycline resistant Escherichia coli and application. The Escherichia coli is preserved in General microbiological center of China microbe preservation management committeeon July 25, 2017, with the preservation number being CGMCC No.14456. The invention further discloses application of the tetracycline resistant Escherichia coli to the preparation of tetracycline resistant products. The Escherichia coli is stable in drug resistance, and can still show drug resistance after half a year in a drug sensitive test performed on the Escherichia coli, and the drug resistant strain is stable and difficult to reverse.

Description

technical field [0001] The application belongs to the field of biotechnology, and in particular relates to a tetracycline-resistant Escherichia coli and its application. Background technique [0002] Escherichia coli disease is a zoonotic disease. It is very common for animals to suffer from Escherichia coli disease. There are many reasons. One of the reasons is that repeated clinical use of the same antibiotic will easily lead to the emergence of drug-resistant strains. Drug-resistant strains make antibiotics Lose antibacterial activity and fail. Therefore, the acquisition of drug-resistant strains is the key to laboratory research on the mechanism of drug resistance and the search for alternative antibacterial drugs, and it is also a necessary material for effectively controlling the occurrence of colibacillosis in farms. [0003] Current studies have shown that the acquisition of drug-resistant strains is mainly through clinical isolation, environmental acquisition, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12Q1/10C12Q1/18A61K36/539A61K36/708A61K36/855A61K36/704A61K36/315A61K36/195A61K36/634A61P31/04C12R1/19
CPCY02A50/30
Inventor 孙裕光沙莎兰仕梅杨溢欢
Owner SOUTHWEST UNIV
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