35 results about "Antimicrobial susceptibility" patented technology

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

Systems and Methods for determining response of microbe cultures to antimicrobials by measuring properties of individual microbes and / or other culture particles. Methods include incubating microbes in liquid cultures which may contain antimicrobials and growth media, detecting individual microbes and particles, and measuring their properties, which may include the mass of individual microbes and particles. Measurement data, such as masses of a plurality of individual microbes or particles, may be collected after an incubation duration. These data may be analyzed to determine susceptibility of microbes to one or more antibiotics. Measurement may be performed after incubation durations shorter than required to produce growth by microbe replication, and analysis may include assessment of metrics other than the growth of the culture. The method enables measurement of susceptibility at earlier times than needed for sufficient microbe replication required by many existing techniques enabling accurate results with relatively minimal sample volumes of a culture.

The invention belongs to the technical field of disease control in aquaculture, and particularly relates to a hippocampus enteritis pathogen separation and identification and medication screening method based on a high-throughput sequencing technology, which comprises the following steps: collecting intestinal tissues of a diseased hippocampus and a healthy hippocampus, respectively preparing tissue homogenate, and freezing for preservation; the method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of microorganisms of an intestinal tissue sample, performing 16SrDNA high-throughput sequencing, analyzing intestinal flora composition, and determining dominant bacteria; selecting and screening a culture medium according to dominant bacteria, and performing pathogenic bacterium separation and purification on intestinal tissues of the diseased hippocampus; dNA of the purified strain is extracted, 16S rDNA amplification and sequencing are carried out, and the species of pathogenic bacteria are identified through sequence comparison; according to the method, the diversity and abundance differences of microorganisms in the sick hippocampus and the healthy hippocampus are compared through a high-throughput sequencing technology, and targeted separation, identification and drug sensitivity screening are further carried out by taking statistically significant difference bacteria as the guidance of potential pathogenic bacteria.

Disclosed is a device for performing growth detection, identification, and antimicrobial susceptibility testing (AST) functions for microbial species to achieve a sample-to-answer result. Methods for performing microbial growth, microbial species identification, and AST using such a device are also disclosed. The device may include a housing configured to receive a sample plate, a sample port configured to receive a sample, a fluid distribution system constructed and arranged to introduce the sample into one or more sample wells of the sample plate, a sample plate imaging system, and a controller configured to collect data from the sample plate imaging system. The controller may detect growth of the microbial species, identify the microbial species, and perform AST on the microbial species once the data is collected.

The present invention belongs to the field of biotechnology. More specifically, it relates to a scalp care composition having the effects of inhibiting bacteria, removing dandruff and relieving itching, and its preparation method and application. In particular, the composition contains the extract of Alpinia katsumadai and piroctone olamine. The extract of Alpinia katsumadai in the present invention has good antibacterial activity against Malassezia globosa. The combined drug sensitivity test shows that the combination of the extract of Alpinia katsumadai and piroctone olamine has a synergistic effect in inhibiting Malassezia globosa.

This application proposes a method and related apparatus for detecting antimicrobial susceptibility based on the physical morphological characteristics of single bacteria. The method includes: S1, establishing an independent detection unit; S2, drug processing; S3, single-bacterial imaging; S4, single-bacterial segmentation; S5, multidimensional feature extraction; S6, single-index performance evaluation; S7, joint modeling and performance evaluation; and S8, result output. This application can establish a rapid drug susceptibility discrimination process for common Gram-negative bacteria in the early single-bacterial morphological changes after antibiotic treatment, thereby improving the interpretability, standardization potential, and clinical translational applicability of the method while shortening the detection time.

The invention relates to the field of drug sensitivity diagnosis, in particular to a swine-derived broad-spectrum enrichment medium as well as a preparation method and application thereof. The pig-derived broad-spectrum enrichment culture medium is prepared from the following raw materials in parts by weight: 5.0 to 10.0 parts of peptone, 3.0 to 8.0 parts of yeast extract powder, 5.0 to 8.0 parts of sodium chloride, 5.0 to 10.0 parts of lactose, 0.5 to 2.5 parts of cholate, 0.002 to 0.005 part of crystal violet, 0.002 to 0.010 part of neutral red, 15.0 to 20.0 parts of agar, 1.0 to 3.5 parts of glucose, 1.0 to 3.5 parts of dipotassium phosphate, 2.5 to 5.0 parts of disodium hydrogen phosphate, 0.2 to 0.5 part of sodium thiosulfate and 1000 parts of distilled water. 50-100 parts of filtered and sterilized newborn calf serum and 1.0-2.5 parts of NAD (Nicotinamide Adenine Dinucleotide) with the mass fraction of 1%. According to the invention, the formula of the enrichment culture medium is optimized, the liquid culture medium is used as the enrichment culture medium for the drug sensitive test, and a new acid-base indicator is selected according to the biochemical characteristics of bacteria, so that the process of the drug sensitive test is simplified.

The invention belongs to the technical field of drug sensitivity detection, and particularly relates to a method for increasing the drug sensitivity detection speed of saccharomycetes and application of the method. The method comprises the steps of bacterial liquid preparation, sample adding, incubation, thallus enrichment, color developing agent adding and result interpretation, ethylenediamine tetraacetic acid or salt thereof needs to be added before thallus enrichment, and drug action and color developing are carried out separately. The detection method provided by the invention can effectively accelerate the developing speed of resazurin, remarkably shortens the drug sensitivity detection time, and is beneficial to early accurate diagnosis and treatment of clinical fungal diseases.

Disclosed in the present invention is a positive blood culture bottle processing device, comprising a feeding and uprighting mechanism, a sterilization mechanism, a code scanning mechanism, a glass slide placement mechanism, a translation and inverting mechanism, a plate placement mechanism, a transfer mechanical arm, an antimicrobial susceptibility disk dispenser, a needle supply mechanism, a plate incubation mechanism, and a plate loading device, wherein the sterilization mechanism and the feeding and uprighting mechanism are spaced apart from each other; the code scanning mechanism and the feeding and uprighting mechanism are spaced apart from each other; the translation and inverting mechanism is arranged on the side of the code scanning mechanism away from the feeding and uprighting mechanism; the plate placement mechanism is arranged on one side of the translation and inverting mechanism in the length direction of the translation and inverting mechanism, and the glass slide placement mechanism is spaced apart from the plate placement mechanism in the length direction of the translation and inverting mechanism; and the transfer mechanical arm is arranged on one side of the feeding and uprighting mechanism, and the transfer mechanical arm is used for moving a positive bottle from the feeding and uprighting mechanism to the code scanning mechanism, moving the positive bottle from the code scanning mechanism to the translation and inverting mechanism, and puncturing the positive bottle, thereby realizing automatic processing of the positive bottle.

Various apparatus, systems, and methods for determining a susceptibility of a bacteria to an antibiotic are disclosed. In one aspect, a reader comprises at least one reader module configured to receive a well plate covered by a sensor array lid. Wells of the well plate contain aliquots of a sample comprising the bacteria. The wells also comprise test wells containing the antibiotic and at least one control well without the antibiotic. The reader module further comprises conductive connectors configured to contact conductive pads of the sensor array lid. One or more processors of the reader are programmed to determine the susceptibility of the bacteria to the antibiotic based on any changes in a solution characteristic of the aliquots within the test wells compared to the control well. The reader is also configured to perform certain internal quality checks when determining the susceptibility of the bacteria to the antibiotic.

Systems and Methods for determining response of microbe cultures to antimicrobials by measuring properties of individual microbes and / or other culture particles. Methods include incubating microbes in liquid cultures which may contain antimicrobials and growth media, detecting individual microbes and particles, and measuring their properties, which may include the mass of individual microbes and particles. Measurement data, such as masses of a plurality of individual microbes or particles, may be collected after an incubation duration. These data may be analyzed to determine susceptibility of microbes to one or more antibiotics. Measurement may be performed after incubation durations shorter than required to produce growth by microbe replication, and analysis may include assessment of metrics other than the growth of the culture. The method enables measurement of susceptibility at earlier times than needed for sufficient microbe replication required by many existing techniques enabling accurate results with relatively minimal sample volumes of a culture.

Devices for performing growth detection, identification and antimicrobial susceptibility testing (AST) functions in connection with a microbial species to achieve sample-to-answer results are disclosed. Methods of performing microbial growth, microbial species identification, and AST using such devices are also disclosed.

A microorganism positive bottle processing apparatus includes an input vertical mechanism, sterilization mechanism, scanning mechanism, glass slide placement mechanism, translation and turning mechanism, plate placement mechanism, transferring robotic arm, antimicrobial susceptibility disc dispenser, needle supply mechanism, plate incubation mechanism and plate loading apparatus. The sterilization mechanism is spaced from the input vertical mechanism; the scanning mechanism is spaced from the input vertical mechanism; the translation and turning mechanism is on the scanning mechanism remote side; the plate placement mechanism is on one side of the translation and turning mechanism, and the glass slide placement mechanism is spaced from the plate placement mechanism lengthwise from the translation and turning mechanism; the transferring robotic arm is on one side of the input vertical mechanism; the transferring robotic arm moves a positive bottle from the input vertical mechanism to the scanning mechanism, then to the translation and turning mechanism, and for puncturing.

The present document relates to a calorimetric method for determining the antimicrobial sensitivity of microorganisms in a sample, such as a clinical tissue sample. According to the method, a microbial sample is exposed to an antibacterial agent when the sample has reached a specific metabolic activity and differences in calorimetric signal (s) are compared relative to the sample incubated in the absence of the antibacterial agent to determine whether the microorganism (s) in the sample are sensitive or insensitive to the antibacterial agent (s).

A method for identifying a pathogen includes obtaining a biological specimen. The method may include dividing the specimen into at least a first sample and a second sample. The method may include identifying at least one pathogen in the first sample via genetic analysis. The method may include exposing the second sample to a panel of antimicrobial agents at known concentrations based on the at least one pathogen. The method may include performing a polymerase chain reaction (PCR) assay on the second sample after exposure of the second sample to the panel of the antimicrobial agents, wherein the PCR assay includes oligonucleotides that amplify a nucleic acid sequence for one or more of the identified pathogens. The method may include analyzing a result of the PCR assay.

Methods and systems for antibacterial susceptibility testing of a bacterium are provided. The method includes exposing a bacterium to an antimicrobial agent. A series of images of the bacterium is captured over time after exposure The series of images are captured during an imaging period. For each image of the series of images, the method includes extracting a value of each feature in a set of morphological features of the bacterium. The set of morphological features includes one or more of area, aspect ratio, length, circularity, perimeter, angularity, curvature, ferret, pole, roundness, sinuosity, width, trajectory, morphology, orientation, solidity, and z-score. A rate of change is calculated for each feature of the set of morphological features during the imaging period. An inhibition status of the bacterium is determined using a machine-learning classifier applied to input data.

Various apparatus, systems, and methods for determining a susceptibility of an infectious agent to an anti-infective are disclosed. In one aspect, a system comprises a well plate, a sensor array lid configured to cover the well plate, and a reader for receiving the well plate covered by the sensor array lid. The well plate comprises wells including test wells comprising an anti-infective and at least one control well devoid of the anti-infective. Each of the wells is configured to receive and contain a sample. The sensor array lid comprises a plurality of sensor units. Each of the sensor units is configured to extend into a well of the well plate. Each of the sensor units comprises an active electrode and a reference electrode used by the reader to detect any changes in the solution characteristics within the wells to determine the susceptibility of the infectious agent to the anti-infective.

The invention relates to a phase-change space-limited microcarrier coupled with a microdroplet digital CRISPR (clustered regularly interspaced short palindromic repeats) technology, a preparation method of the phase-change space-limited microcarrier, a detection method of drug sensitivity and application of the phase-change space-limited microcarrier coupled with the microdroplet digital CRISPR technology. The kit is used for realizing on-demand separation and mixing of recombinase-assisted amplification (RAA) and CRISPR / Cas12a reaction, so that genome DNA copy number change of bacteria after drug exposure is efficiently detected, and rapid and accurate antibacterial drug sensitivity test is realized. The space-limited microcarrier prepared by the invention is low in cost, diversified in function, simple in instrument and operation, and suitable for popularization. The detection method can be effectively and conveniently applied to multiple fields of health and sanitation, public safety and the like, and has profound social and clinical values.

This invention proposes a method, system, device, and storage medium for drug susceptibility detection based on single-bacterial segmentation and deep learning. The method includes: acquiring bacterial microscopic images and fusing their bright-field and fluorescence channels to obtain a fused microscopic image; delineating and masking single bacteria in the fused image to form a single-bacterial mask dataset; using this dataset for supervised training based on a single-cell segmentation framework, and iteratively optimizing to obtain an optimized segmentation model; using the optimized segmentation model to automatically segment bacterial images and adaptively adjust thresholds, extracting single-bacterial regions and performing quality control to obtain a qualified single-bacterial image set; performing hierarchical multi-fold cross-validation on this image set, training multiple deep learning models, and selecting a core model based on the average performance of the validation set; predicting the image of the target bacterial strain using the core model and outputting the drug susceptibility detection result. This invention can rapidly identify the drug susceptibility status of bacteria, improving detection accuracy and stability.

Provided is a method for preparing a bacterial liquid for antimicrobial susceptibility testing. The method comprises: picking a bacterial colony from a sample for preparing the bacterial liquid; preparing the bacterial colony into a first bacterial liquid, and counting bacteria in the first bacterial liquid to obtain a first bacterial count of the first bacterial liquid; and deducing a first concentration of the first bacterial liquid on the basis of the first bacterial count, and diluting the first bacterial liquid on the basis of the first concentration to obtain a second bacterial liquid, wherein the second concentration of the second bacterial liquid is 105 to 106 / milliliters (cfu / mL); and the second bacterial liquid is the bacterial liquid for the antimicrobial susceptibility testing. By applying a counting method to the preparation of the bacterial liquid for the antimicrobial susceptibility testing, the time for preparing the bacterial liquid is greatly shortened, and the concentration of the sample used is almost lower than 100 times a bacterial liquid amount of 0.5MCF (1.5*108cfu / mL). That is to say, a bacterial liquid for antimicrobial susceptibility testing can be prepared at a low concentration, thus achieving an application innovation.

The present invention belongs to the technical field of bacterial drug resistance analysis, and specifically relates to an antimicrobial drug screening method based on bacterial drug resistance gene promoter strength detection, which includes collecting and isolating drug-resistant strains; determining the drug resistance gene of the strain and its upstream promoter sequence; performing minimum inhibitory concentration value detection based on drug sensitivity experiments; establishing a mathematical analysis model for bacterial drug resistance analysis, performing boundary mathematical representation of the minimum inhibitory concentrations of drug resistance and non-drug resistance; performing a three-dimensional matrix representation of the strain drug sensitivity test results; performing an indicator function representation of the strain drug sensitivity test results; performing dimensionality reduction processing on the three-dimensional matrix of the strain drug sensitivity test results; screening for non-drug-resistant antimicrobial drugs for strains carrying any one drug resistance gene; screening for non-drug-resistant antimicrobial drugs for strains carrying any multiple drug resistance genes, and completing bacterial resistance analysis and antimicrobial drug screening. The present invention more conveniently and effectively guides clinical selection of antimicrobial drugs for drug-resistant bacteria.

Provided are systems, devices, kits, and methods that are used for antimicrobial sensitivity testing (AST). The systems, devices, kits, and methods provide a capillary-based antimicrobial susceptibility testing platform referred to as “cAST” that provides faster determination of antimicrobial susceptibility using lower sample volumes than previous approaches. The cAST approach includes performing AST by measuring colorimetric or fluorescent signal changes in samples in conduits that contain antimicrobial agents and one or more dyes. The conduits are operably linked to a light source and a light detecting component through a mount configured to hold the conduits and thereby determine changes in light that indicates changes in bacteria growth in the present of the antimicrobial agents.

The disclosure provides cartridges that are pre-loaded with reagents for performing antimicrobial susceptibility testing (AST) and FISH testing. Cartridges of the disclosure include various incubation wells loaded with different antimicrobial agents for differential growth analysis. Imaging wells with species-specific microbial probes, fluorescent tags, magnetic particles and dye-cushion layers allow for tagging and imaging of target microbes for differential growth analysis.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and / or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and / or determine antimicrobial susceptibility of such microorganisms.

Various apparatus, systems, and methods for determining a susceptibility of a bacteria to an antibiotic are disclosed. In one aspect, a reader comprises at least one reader module configured to receive a well plate covered by a sensor array lid. Wells of the well plate contain aliquots of a sample comprising the bacteria. The wells also comprise test wells containing the antibiotic and at least one control well without the antibiotic. The reader module further comprises conductive connectors configured to contact conductive pads of the sensor array lid. One or more processors of the reader are programmed to determine the susceptibility of the bacteria to the antibiotic based on any changes in a solution characteristic of the aliquots within the test wells compared to the control well. The reader is also configured to perform certain internal quality checks when determining the susceptibility of the bacteria to the antibiotic.

The invention provides a micro-fluidic chip and a method for detecting drug sensitivity of bacteria. The micro-fluidic chip comprises at least one detection area, and the detection area comprises at least one drug reaction tank in which an antibacterial drug is pre-buried; an antibacterial drug is not embedded in the positive control tank; wherein the drug reaction tank and the positive control tank are respectively in fluid communication with respective fluorescence detection tanks through hydrophobic barrier channels, and a reagent and a fluorescent dye which are used for cracking bacteria are pre-embedded in the fluorescence detection tanks. According to the micro-fluidic drug sensitive chip provided by the invention, the reaction of bacteria and drugs and fluorescence detection are orderly integrated together, through the structural arrangement of the micro-fluidic drug sensitive chip and the corresponding operation mode, the operation is simple, convenient and rapid when a drug sensitive test and fluorescence detection are carried out, and the probability of aerosol and cross contamination can be greatly reduced.

The invention discloses a probiotic A001-A-08 with a cholesterol lowering function, which is derived from a local characteristic food, namely, lactarian milkvetch, of Dali, and is identified as lactobacillus fermentum in phylogenetics. The probiotic A001-A-08 has the advantages that the probiotic A001-A-08 has the cholesterol lowering function, and the probiotic A001-A-08 is prepared from lactobacillus fermentum; in-vitro function evaluation shows that the strain forms a clear precipitation ring on a TDCA / CaCl indication plate, and the activity of bile salt hydrolase is positive; the surface hydrophobicity is higher, and the self-aggregation property is good; in the aspect of tolerance, A001-A-08 still can survive and keep high activity (greater than 99%) in the presence of an acidic condition (pH 2.5-3.0) and sodium taurocholate (about 0.03-0.3%, w / v), and shows a relatively high survival rate in a simulated gastric juice / intestinal juice system; a paper diffusion method drug sensitive test shows that most common antibiotics have a definite inhibition effect on the strain; alpha / beta hemolysis does not appear on 5% Columbia blood agar, and the alpha / beta hemolysis is represented as gamma hemolysis, so that the probiotic safety requirement is met. The strain is safe in source, resistant to acid and bile salt, good in adhesion potential, suitable for development of functional fermented food, dairy products and nutritional and healthy products and capable of achieving the dual health value of'probiotic and lipid regulation '.

The invention provides a rapid microorganism drug sensitivity detection method, application and a product, and belongs to the technical field of microorganism drug sensitivity detection. The method comprises the following steps: S1, carrying out microbiological treatment to obtain a sample bacteria solution; s2, adding a to-be-detected medicine into the sample bacterial liquid, and incubating; s3, after incubation, enriching the thalli; and S4, adding a color developing agent to observe the color change. According to the method, drug sensitivity data can be obtained within several hours, the drug sensitivity detection speed is increased, and the method is suitable for drug sensitivity detection of pure cultures of bacteria and fungi and also suitable for drug sensitivity detection of blood culture positive samples, urine samples and other samples with high bacterium content. The method is of great significance in rapidly guiding clinicians to reasonably take medicines, reducing the death rate of infected patients and saving the lives of the patients.