6 results about "Antimicrobial susceptibility" patented technology

This application proposes a method and related apparatus for detecting antimicrobial susceptibility based on the physical morphological characteristics of single bacteria. The method includes: S1, establishing an independent detection unit; S2, drug processing; S3, single-bacterial imaging; S4, single-bacterial segmentation; S5, multidimensional feature extraction; S6, single-index performance evaluation; S7, joint modeling and performance evaluation; and S8, result output. This application can establish a rapid drug susceptibility discrimination process for common Gram-negative bacteria in the early single-bacterial morphological changes after antibiotic treatment, thereby improving the interpretability, standardization potential, and clinical translational applicability of the method while shortening the detection time.

This invention proposes a method, system, device, and storage medium for drug susceptibility detection based on single-bacterial segmentation and deep learning. The method includes: acquiring bacterial microscopic images and fusing their bright-field and fluorescence channels to obtain a fused microscopic image; delineating and masking single bacteria in the fused image to form a single-bacterial mask dataset; using this dataset for supervised training based on a single-cell segmentation framework, and iteratively optimizing to obtain an optimized segmentation model; using the optimized segmentation model to automatically segment bacterial images and adaptively adjust thresholds, extracting single-bacterial regions and performing quality control to obtain a qualified single-bacterial image set; performing hierarchical multi-fold cross-validation on this image set, training multiple deep learning models, and selecting a core model based on the average performance of the validation set; predicting the image of the target bacterial strain using the core model and outputting the drug susceptibility detection result. This invention can rapidly identify the drug susceptibility status of bacteria, improving detection accuracy and stability.

The present invention relates to compositions and methods for the use of polymerase chain reaction (PCR) as a reporter assay for rapid and simultaneous bacterial identification and phenotype testing for antimicrobial susceptibility (AST). The current invention uses a strategy that has shown the ability for multiplexing and for handling polymicrobial samples for antimicrobial susceptibility testing.

This invention relates to the field of microbial detection technology and discloses a partitioned antimicrobial susceptibility testing plate and its application. The plate comprises a plate body and a plurality of microwell reaction units disposed on the plate body. The plate body is spatially divided into at least two independent detection zones, including a first detection zone and a second detection zone. A first selective culture system is pre-installed within the microwell reaction units of the first detection zone. The first selective culture system includes neomycin and at least one antimicrobial drug concentration gradient targeting Gram-negative bacilli. The partitioned antimicrobial susceptibility testing plate provided by this invention enables direct and simultaneous antimicrobial susceptibility testing of mixed samples of Gram-negative bacilli and Gram-positive cocci.