49 results about "Antimicrobial susceptibility" patented technology

According to the device and the reagent for extracting the pathogen, the pathogen product separated from the uncultured sample extracted by using the device and the reagent can be subjected to a drug sensitivity test after simple culture and bacteria collection treatment, so that the culture process is shortened compared with pathogen diagnosis and identification of a clinical infected sample, and a drug sensitivity result can be obtained more quickly; and a more timely diagnosis and treatment reference is provided for clinicians.

Described herein are systems, methods, and devices for antimicrobial susceptibility testing (AST) directly from blood samples. A system for enriching samples includes a housing configured to receive sample containers, sample preparation cartridges, and AST cartridges, for processing and testing samples using one or more centrifuges, pipetting systems, a controller, AST subsystem, magnet station, microscope, and heater disposed inside the housing. The system is configured to transfer a sample from a blood sample container into a processing tube in a sample preparation cartridge using a septum and needle-based pipetting system. Upon transfer of the sample, the system performs steps to separate, enrich, and concentrate pathogens in the sample for rapid detection. The system then dispenses aliquots of the enriched sample into antimicrobial-containing reaction wells in an AST cartridge, acquires images of each reaction well after incubation, and determines pathogen susceptibility to antimicrobials based on analyzing the acquired images.

Systems and Methods for determining response of microbe cultures to antimicrobials by measuring properties of individual microbes and / or other culture particles. Methods include incubating microbes in liquid cultures which may contain antimicrobials and growth media, detecting individual microbes and particles, and measuring their properties, which may include the mass of individual microbes and particles. Measurement data, such as masses of a plurality of individual microbes or particles, may be collected after an incubation duration. These data may be analyzed to determine susceptibility of microbes to one or more antibiotics. Measurement may be performed after incubation durations shorter than required to produce growth by microbe replication, and analysis may include assessment of metrics other than the growth of the culture. The method enables measurement of susceptibility at earlier times than needed for sufficient microbe replication required by many existing techniques enabling accurate results with relatively minimal sample volumes of a culture.

The invention belongs to the technical field of disease control in aquaculture, and particularly relates to a hippocampus enteritis pathogen separation and identification and medication screening method based on a high-throughput sequencing technology, which comprises the following steps: collecting intestinal tissues of a diseased hippocampus and a healthy hippocampus, respectively preparing tissue homogenate, and freezing for preservation; the method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of microorganisms of an intestinal tissue sample, performing 16SrDNA high-throughput sequencing, analyzing intestinal flora composition, and determining dominant bacteria; selecting and screening a culture medium according to dominant bacteria, and performing pathogenic bacterium separation and purification on intestinal tissues of the diseased hippocampus; dNA of the purified strain is extracted, 16S rDNA amplification and sequencing are carried out, and the species of pathogenic bacteria are identified through sequence comparison; according to the method, the diversity and abundance differences of microorganisms in the sick hippocampus and the healthy hippocampus are compared through a high-throughput sequencing technology, and targeted separation, identification and drug sensitivity screening are further carried out by taking statistically significant difference bacteria as the guidance of potential pathogenic bacteria.

Disclosed is a device for performing growth detection, identification, and antimicrobial susceptibility testing (AST) functions for microbial species to achieve a sample-to-answer result. Methods for performing microbial growth, microbial species identification, and AST using such a device are also disclosed. The device may include a housing configured to receive a sample plate, a sample port configured to receive a sample, a fluid distribution system constructed and arranged to introduce the sample into one or more sample wells of the sample plate, a sample plate imaging system, and a controller configured to collect data from the sample plate imaging system. The controller may detect growth of the microbial species, identify the microbial species, and perform AST on the microbial species once the data is collected.

A method for performing antimicrobial susceptibility testing, comprising activating protein biosynthesis in microorganisms obtained from a biological sample in an acclimatization buffer; exposing the microorganisms to a library that includes various antimicrobials at predetermined concentrations, wherein exposure either kills the microorganisms or blocks protein biosynthesis in the microorganisms that are sensitive to one or more of the antimicrobials at one or more of the predetermined concentrations; labeling newly biosynthesized proteins produced by the microorganisms that survive exposure to the antimicrobials with a non-canonical amino acid (ncAA); tagging the labeled proteins with a detectable element attached to the ncAA, to create an amount of detectable signal; and comparing the amount of detected signal to a positive control, wherein an absence of or a decrease in the amount of signal relative to the positive control indicates effectiveness of one or more of the antimicrobials at one or more of the predetermined concentrations.

The present invention belongs to the field of biotechnology. More specifically, it relates to a scalp care composition having the effects of inhibiting bacteria, removing dandruff and relieving itching, and its preparation method and application. In particular, the composition contains the extract of Alpinia katsumadai and piroctone olamine. The extract of Alpinia katsumadai in the present invention has good antibacterial activity against Malassezia globosa. The combined drug sensitivity test shows that the combination of the extract of Alpinia katsumadai and piroctone olamine has a synergistic effect in inhibiting Malassezia globosa.

This application proposes a method and related apparatus for detecting antimicrobial susceptibility based on the physical morphological characteristics of single bacteria. The method includes: S1, establishing an independent detection unit; S2, drug processing; S3, single-bacterial imaging; S4, single-bacterial segmentation; S5, multidimensional feature extraction; S6, single-index performance evaluation; S7, joint modeling and performance evaluation; and S8, result output. This application can establish a rapid drug susceptibility discrimination process for common Gram-negative bacteria in the early single-bacterial morphological changes after antibiotic treatment, thereby improving the interpretability, standardization potential, and clinical translational applicability of the method while shortening the detection time.

The present disclosure relates to a microfluidic circuit comprising a drug inlet port; an outlet port; a drug inlet main channel fluidically connecting the drug inlet port and the outlet port, where said drug inlet main channel comprises (i) a plurality of serpentine mixers and (ii) a plurality of dead-end first microchamber sets; a negative inlet port; a negative inlet main channel fluidically connecting the negative inlet port and the drug inlet main channel; a plurality (n) of ladder channels, where each of the plurality (n) of the ladder channels is fluidically connected to both the drug inlet main channel and the negative inlet main channel; and an outlet channel fluidically connected to the drug inlet main channel between the drug inlet port and the outlet port. Also disclosed is a microfluidic device comprising a microfluidic circuit of the present disclosure and a method for performing an assay.

The invention relates to the field of drug sensitivity diagnosis, in particular to a swine-derived broad-spectrum enrichment medium as well as a preparation method and application thereof. The pig-derived broad-spectrum enrichment culture medium is prepared from the following raw materials in parts by weight: 5.0 to 10.0 parts of peptone, 3.0 to 8.0 parts of yeast extract powder, 5.0 to 8.0 parts of sodium chloride, 5.0 to 10.0 parts of lactose, 0.5 to 2.5 parts of cholate, 0.002 to 0.005 part of crystal violet, 0.002 to 0.010 part of neutral red, 15.0 to 20.0 parts of agar, 1.0 to 3.5 parts of glucose, 1.0 to 3.5 parts of dipotassium phosphate, 2.5 to 5.0 parts of disodium hydrogen phosphate, 0.2 to 0.5 part of sodium thiosulfate and 1000 parts of distilled water. 50-100 parts of filtered and sterilized newborn calf serum and 1.0-2.5 parts of NAD (Nicotinamide Adenine Dinucleotide) with the mass fraction of 1%. According to the invention, the formula of the enrichment culture medium is optimized, the liquid culture medium is used as the enrichment culture medium for the drug sensitive test, and a new acid-base indicator is selected according to the biochemical characteristics of bacteria, so that the process of the drug sensitive test is simplified.

The invention belongs to the technical field of drug sensitivity detection, and particularly relates to a method for increasing the drug sensitivity detection speed of saccharomycetes and application of the method. The method comprises the steps of bacterial liquid preparation, sample adding, incubation, thallus enrichment, color developing agent adding and result interpretation, ethylenediamine tetraacetic acid or salt thereof needs to be added before thallus enrichment, and drug action and color developing are carried out separately. The detection method provided by the invention can effectively accelerate the developing speed of resazurin, remarkably shortens the drug sensitivity detection time, and is beneficial to early accurate diagnosis and treatment of clinical fungal diseases.

Disclosed in the present invention is a positive blood culture bottle processing device, comprising a feeding and uprighting mechanism, a sterilization mechanism, a code scanning mechanism, a glass slide placement mechanism, a translation and inverting mechanism, a plate placement mechanism, a transfer mechanical arm, an antimicrobial susceptibility disk dispenser, a needle supply mechanism, a plate incubation mechanism, and a plate loading device, wherein the sterilization mechanism and the feeding and uprighting mechanism are spaced apart from each other; the code scanning mechanism and the feeding and uprighting mechanism are spaced apart from each other; the translation and inverting mechanism is arranged on the side of the code scanning mechanism away from the feeding and uprighting mechanism; the plate placement mechanism is arranged on one side of the translation and inverting mechanism in the length direction of the translation and inverting mechanism, and the glass slide placement mechanism is spaced apart from the plate placement mechanism in the length direction of the translation and inverting mechanism; and the transfer mechanical arm is arranged on one side of the feeding and uprighting mechanism, and the transfer mechanical arm is used for moving a positive bottle from the feeding and uprighting mechanism to the code scanning mechanism, moving the positive bottle from the code scanning mechanism to the translation and inverting mechanism, and puncturing the positive bottle, thereby realizing automatic processing of the positive bottle.

Various apparatus, systems, and methods for determining a susceptibility of a bacteria to an antibiotic are disclosed. In one aspect, a reader comprises at least one reader module configured to receive a well plate covered by a sensor array lid. Wells of the well plate contain aliquots of a sample comprising the bacteria. The wells also comprise test wells containing the antibiotic and at least one control well without the antibiotic. The reader module further comprises conductive connectors configured to contact conductive pads of the sensor array lid. One or more processors of the reader are programmed to determine the susceptibility of the bacteria to the antibiotic based on any changes in a solution characteristic of the aliquots within the test wells compared to the control well. The reader is also configured to perform certain internal quality checks when determining the susceptibility of the bacteria to the antibiotic.

Systems and Methods for determining response of microbe cultures to antimicrobials by measuring properties of individual microbes and / or other culture particles. Methods include incubating microbes in liquid cultures which may contain antimicrobials and growth media, detecting individual microbes and particles, and measuring their properties, which may include the mass of individual microbes and particles. Measurement data, such as masses of a plurality of individual microbes or particles, may be collected after an incubation duration. These data may be analyzed to determine susceptibility of microbes to one or more antibiotics. Measurement may be performed after incubation durations shorter than required to produce growth by microbe replication, and analysis may include assessment of metrics other than the growth of the culture. The method enables measurement of susceptibility at earlier times than needed for sufficient microbe replication required by many existing techniques enabling accurate results with relatively minimal sample volumes of a culture.

The invention relates to methods and kits for the detection and determination of the susceptibility of microorganisms to therapeutic agents. The invention relates to the rapid determination of bacterial susceptibility to antibiotics. The invention also extends to methods of sample preparation and microorganism extraction from a biological sample, and to methods of identifying microorganisms, and to kits and panels specifically designed to determine the susceptibility of extracted microorganisms to antimicrobial agents. The invention is especially useful in clinical diagnostic and veterinary medicine.

Devices for performing growth detection, identification and antimicrobial susceptibility testing (AST) functions in connection with a microbial species to achieve sample-to-answer results are disclosed. Methods of performing microbial growth, microbial species identification, and AST using such devices are also disclosed.

The present invention concerns a method of measuring antimicrobial susceptibility of microbes using a suitable device or system. Also provided are a device and a system suitable for measuring antimicrobial susceptibility of microbes and use of the device and system to measure the antimicrobial susceptibility of microbes.

A microorganism positive bottle processing apparatus includes an input vertical mechanism, sterilization mechanism, scanning mechanism, glass slide placement mechanism, translation and turning mechanism, plate placement mechanism, transferring robotic arm, antimicrobial susceptibility disc dispenser, needle supply mechanism, plate incubation mechanism and plate loading apparatus. The sterilization mechanism is spaced from the input vertical mechanism; the scanning mechanism is spaced from the input vertical mechanism; the translation and turning mechanism is on the scanning mechanism remote side; the plate placement mechanism is on one side of the translation and turning mechanism, and the glass slide placement mechanism is spaced from the plate placement mechanism lengthwise from the translation and turning mechanism; the transferring robotic arm is on one side of the input vertical mechanism; the transferring robotic arm moves a positive bottle from the input vertical mechanism to the scanning mechanism, then to the translation and turning mechanism, and for puncturing.

The present document relates to a calorimetric method for determining the antimicrobial sensitivity of microorganisms in a sample, such as a clinical tissue sample. According to the method, a microbial sample is exposed to an antibacterial agent when the sample has reached a specific metabolic activity and differences in calorimetric signal (s) are compared relative to the sample incubated in the absence of the antibacterial agent to determine whether the microorganism (s) in the sample are sensitive or insensitive to the antibacterial agent (s).

A method for identifying a pathogen includes obtaining a biological specimen. The method may include dividing the specimen into at least a first sample and a second sample. The method may include identifying at least one pathogen in the first sample via genetic analysis. The method may include exposing the second sample to a panel of antimicrobial agents at known concentrations based on the at least one pathogen. The method may include performing a polymerase chain reaction (PCR) assay on the second sample after exposure of the second sample to the panel of the antimicrobial agents, wherein the PCR assay includes oligonucleotides that amplify a nucleic acid sequence for one or more of the identified pathogens. The method may include analyzing a result of the PCR assay.

Systems and methods for quickly determining antibacterial or antimicrobial susceptibility using an innovative growth dynamic model are disclosed. A system includes an image collection subsystem constructed and arranged to generate a plurality of images of a microbial sample. The system also includes an image analysis subsystem comprising a non-transitory computer-readable medium storing thereon sequences of computer-executable instructions for determining the susceptibility of the microbial species from the image of the microbial sample that, when executed by one or more processors, cause the one or more processors to perform operations that calculate the susceptibility of the microbial species by determining one or both of microbial species replication and microbial species stasis in the presence of the antimicrobial agent from the manipulation of the changing pixel intensities. A non-transitory computer-readable medium storing thereon instructions for determining antibacterial or antimicrobial susceptibility are also disclosed.

Methods and systems for antibacterial susceptibility testing of a bacterium are provided. The method includes exposing a bacterium to an antimicrobial agent. A series of images of the bacterium is captured over time after exposure The series of images are captured during an imaging period. For each image of the series of images, the method includes extracting a value of each feature in a set of morphological features of the bacterium. The set of morphological features includes one or more of area, aspect ratio, length, circularity, perimeter, angularity, curvature, ferret, pole, roundness, sinuosity, width, trajectory, morphology, orientation, solidity, and z-score. A rate of change is calculated for each feature of the set of morphological features during the imaging period. An inhibition status of the bacterium is determined using a machine-learning classifier applied to input data.

Various apparatus, systems, and methods for determining a susceptibility of an infectious agent to an anti-infective are disclosed. In one aspect, a system comprises a well plate, a sensor array lid configured to cover the well plate, and a reader for receiving the well plate covered by the sensor array lid. The well plate comprises wells including test wells comprising an anti-infective and at least one control well devoid of the anti-infective. Each of the wells is configured to receive and contain a sample. The sensor array lid comprises a plurality of sensor units. Each of the sensor units is configured to extend into a well of the well plate. Each of the sensor units comprises an active electrode and a reference electrode used by the reader to detect any changes in the solution characteristics within the wells to determine the susceptibility of the infectious agent to the anti-infective.

To provide a multi-function instrument for automatically and simultaneously carrying out a variety of tests including identification of targets in specimens and antimicrobial susceptibility testing thereof.SOLUTION: Application-specific cartridges are pre-loaded with all required reagents and allow tests to be performed in a single testing device without specimen preparation to, for example, rapidly detect infections, identify infectious pathogens, and analyze their susceptibility to various antimicrobial agents. An instrument includes various stations for carrying out test steps that can be randomly accessed in any order instructed by a computer-controlled instrument.SELECTED DRAWING: None

An excellent drug that increases the antimicrobial susceptibility of multidrug-resistant Pseudomonas aeruginosa. A VHH antibody or a dimer or trimer of the VHH antibody that recognizes loop 1 or loop 2 in OprM of an RND-type multidrug efflux pump of Pseudomonas aeruginosa.

The invention relates to a phase-change space-limited microcarrier coupled with a microdroplet digital CRISPR (clustered regularly interspaced short palindromic repeats) technology, a preparation method of the phase-change space-limited microcarrier, a detection method of drug sensitivity and application of the phase-change space-limited microcarrier coupled with the microdroplet digital CRISPR technology. The kit is used for realizing on-demand separation and mixing of recombinase-assisted amplification (RAA) and CRISPR / Cas12a reaction, so that genome DNA copy number change of bacteria after drug exposure is efficiently detected, and rapid and accurate antibacterial drug sensitivity test is realized. The space-limited microcarrier prepared by the invention is low in cost, diversified in function, simple in instrument and operation, and suitable for popularization. The detection method can be effectively and conveniently applied to multiple fields of health and sanitation, public safety and the like, and has profound social and clinical values.

The disclosure provides cartridges that are pre-loaded with reagents for performing antimicrobial susceptibility testing (AST) and FISH testing. Cartridges of the disclosure include various incubation wells loaded with different antimicrobial agents for differential growth analysis. Imaging wells with species-specific microbial probes, fluorescent tags, magnetic particles and dye-cushion layers allow for tagging and imaging of target microbes for differential growth analysis.

This invention proposes a method, system, device, and storage medium for drug susceptibility detection based on single-bacterial segmentation and deep learning. The method includes: acquiring bacterial microscopic images and fusing their bright-field and fluorescence channels to obtain a fused microscopic image; delineating and masking single bacteria in the fused image to form a single-bacterial mask dataset; using this dataset for supervised training based on a single-cell segmentation framework, and iteratively optimizing to obtain an optimized segmentation model; using the optimized segmentation model to automatically segment bacterial images and adaptively adjust thresholds, extracting single-bacterial regions and performing quality control to obtain a qualified single-bacterial image set; performing hierarchical multi-fold cross-validation on this image set, training multiple deep learning models, and selecting a core model based on the average performance of the validation set; predicting the image of the target bacterial strain using the core model and outputting the drug susceptibility detection result. This invention can rapidly identify the drug susceptibility status of bacteria, improving detection accuracy and stability.

A method for performing antimicrobial susceptibility testing, comprising activating protein biosynthesis in microorganisms obtained from a biological sample in an acclimatization buffer; exposing the microorganisms to a library that includes various antimicrobials at predetermined concentrations, wherein exposure either kills the microorganisms or blocks protein biosynthesis in the microorganisms that are sensitive to one or more of the antimicrobials at one or more of the predetermined concentrations; labeling newly biosynthesized proteins produced by the microorganisms that survive exposure to the antimicrobials with a non-canonical amino acid (ncAA); tagging the labeled proteins with a detectable element attached to the ncAA, to create an amount of detectable signal; and comparing the amount of detected signal to a positive control, wherein an absence of or a decrease in the amount of signal relative to the positive control indicates effectiveness of one or more of the antimicrobials at one or more of the predetermined concentrations.