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Drug-sensitive kit and preparation method thereof and bacterial drug sensitivity testing method

A technology for detecting kits and bacteria, applied in biochemical equipment and methods, microorganism-based methods, measuring devices, etc., can solve the problems of cumbersome operation, many consumption materials, outdated and single types of drugs, etc.

Active Publication Date: 2020-02-28
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a drug susceptibility kit and its preparation method and bacterial drug susceptibility detection method, which solves the problems of outdated and single types of drugs, cumbersome operations, and excessive consumption of materials during mass detection in commercially available drug susceptibility detection products. Many problems; the present invention has the characteristics of improving detection accuracy, large sample size in a single detection, and is suitable for veterinary clinics, and can meet the detection requirements of various types of bacteria

Method used

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  • Drug-sensitive kit and preparation method thereof and bacterial drug sensitivity testing method
  • Drug-sensitive kit and preparation method thereof and bacterial drug sensitivity testing method
  • Drug-sensitive kit and preparation method thereof and bacterial drug sensitivity testing method

Examples

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Effect test

Embodiment 1

[0067] A veterinary bacterial drug susceptibility detection kit, comprising a drug susceptibility plate, a liquid medium and a solid medium plate;

[0068] The drug sensitive plate includes a plate body and a plate cover; the plate body includes a detection hole, a positive control well, a negative control well and a blank control well; Negative control wells and blank control wells.

[0069] Drug sensitivity panels include Escherichia coli, Staphylococcus aureus, and Streptococcus;

[0070] Escherichia coli susceptibility plate coated with amoxicillin, florfenicol, tilmicosin, enrofloxacin, ceftiofur sodium, oxytetracycline, streptomycin sulfate, neomycin sulfate, doxycycline Trimethoprim, Ciprofloxacin;

[0071] Staphylococcus aureus susceptibility plate coated with penicillin, florfenicol, tilmicosin, enrofloxacin, ceftiofur sodium, streptomycin sulfate, neomycin sulfate, doxycycline, trimethoprim , lincomycin, tylosin;

[0072] Streptococcal susceptibility plate coated...

Embodiment 2

[0077] A method for preparing a veterinary bacterial drug susceptibility detection kit, comprising preparing a drug susceptibility plate, preparing a liquid medium and preparing a solid medium plate;

[0078] The steps for preparing the drug-sensitivity plate are as follows: add the drug suitable for the bacterial drug-sensitivity test and the anti-oxidation coating liquid to the drug-sensitivity plate, heat-dry and vacuum-pack;

[0079] The drug sensitivity plate is a 96-well plate, as follows:

[0080]

[0081] Among them, the A1-H11 area is the detection well, and each column is coated with different drugs suitable for bacterial drug susceptibility testing; A12-B12 is the blank control well, and liquid LB medium is added; D12-E12 is the negative control well, and the inactivated waiting Test bacteria solution; G12-H12 is the positive control well, add the test bacteria solution;

[0082] Positive control: For the detection requirements of multiple strains, you can mix t...

Embodiment 3

[0105] A bacterial drug susceptibility detection method, using the above kit for detection, the specific steps are as follows:

[0106] (1) Aseptically collect the diseased material and inoculate the diseased tissue into the corresponding medium plate, inoculate the bacterial sample in the diseased tissue onto the medium plate, place the inoculated plate at 36-38°C for culture, and observe the bacteria after 12-16 hours Growth form, and pick colonies with morphological characteristics that meet the requirements and inoculate them in liquid LB medium, culture at 36-38°C, 160-180rpm, and obtain bacterial liquid after 10-12 hours;

[0107] (2) Use liquid LB medium to dilute the bacterial solution, adjust the absorbance value of the bacterial solution at 630nm to 0.2-0.4, then take 200 μL of bacterial solution and mix it with 9.8 mL of liquid LB medium to obtain a bacterial concentration of about 1×10 6 The initial bacterial solution for drug susceptibility testing;

[0108] (3) ...

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Abstract

The invention belongs to the technical field of drug testing and discloses a drug-sensitive kit and a preparation method thereof and a bacterial drug sensitivity testing method. The kit comprises a drug-sensitive plate, liquid culture media and solid culture medium panels. Conventional and latest veterinary drugs are selected for making the drug-sensitive plate, and the different culture medium panels are prepared according to different infection bacteria. The prepared drug-sensitive kit has advantages of accurate testing results, low time consumption, simplicity and convenience in operation and low cost and is suitable for drug sensitivity testing for bacterial diseases in large-scale farms and basic veterinary institutions, and accordingly accuracy in animal medication can be realized.

Description

technical field [0001] The invention relates to the technical field of drug detection, in particular to a drug sensitivity kit, a preparation method thereof, and a bacterial drug sensitivity detection method. Background technique [0002] In animal production, some farms abuse antibiotics. For example, in order to improve the preventive and growth-promoting effects of antibiotics, antibiotics are used for a long time and in excess doses, which leads to serious imbalance of bacteria in animals and accelerates the generation and transmission of drug-resistant strains. . With the improvement of people's food safety awareness and the increasing attention to bacterial drug resistance, many antibiotics have been banned from use in livestock and poultry farming. The cost of treatment slows down the emergence of drug-resistant strains. [0003] The methods currently used for microbial susceptibility testing mainly include disk diffusion method and dilution method. The paper diffu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/19C12R1/445C12R1/46
CPCC12Q1/18G01N2333/245G01N2333/31G01N2333/315Y02A50/30
Inventor 郭抗抗李鑫鑫刘勇张彦明
Owner NORTHWEST A & F UNIV
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