Mycobacterium tuberculosis drug sensitivity test method as well as application and solid culture medium of indicator

Abstract

The invention discloses a mycobacterium tuberculosis drug sensitivity test method, application and a solid culture medium of an indicator for the mycobacterium tuberculosis drug sensitivity test method as well as a heating thermostat unit and a culture box used for the mycobacterium tuberculosis drug sensitivity test method. The drug sensitivity test method thereof comprises the following steps of adding a liquid enriched medium to carry out enriched culture after subjected to pre-treatment on a supplied sample, warming and melting the solid culture medium to prepare a liquid solid culture medium, mixing the sample subjected to enriched culture and the liquid solid culture medium, then cooling and solidifying to prepare a solid drug sensitivity test sample with a drug sensitivity paper scraps, culturing the solid drug sensitivity test sample for 5-15 days, observing a drug inhibition zone through indication of the indicator, and determining the drug sensitivity. The invention has the advantages of short testing process time, little investment, low use cost and safe and convenient use with an average testing time of about 10 days.

Description

technical field [0001] The invention relates to the field of medical testing for testing the drug resistance of Mycobacterium tuberculosis, specifically a method for drug susceptibility testing of Mycobacterium tuberculosis, and the application of an indicator used in the method for drug susceptibility testing of Mycobacterium tuberculosis And solid culture medium, and the heating constant temperature machine and culture box that are used in the method for the drug susceptibility test of Mycobacterium tuberculosis. Background technique [0002] Drug resistance in tuberculosis is a growing public health problem that seriously threatens tuberculosis control. Zhong Nanshan, an academician of the Chinese Academy of Engineering, said during the two sessions that there are 4.5 million active tuberculosis patients in China, and 550 million carriers, and 10% of carriers have a chance of developing the disease in their lifetime. In April 2009, Chen Zhu, Minister of Health of China, ...

AI Technical Summary

Problems solved by technology

The advantages of the existing disc test method are convenient operation, simple equipment, and small investment; but it has the following disadvantages: (1) The time required for the entire test is too long: about 1 month is required for the proliferation or isolation culture stage, and drug sensitivity The inspection stage takes about 1 month, and the whole test process takes about 2 months

If the drug treatment is used after the results of the drug susceptibility test are obtained, it will seriously affect the timing of treatment

In clinical practice, the use of drug treatment without drug susceptibility testing may lead to serious consequences of drug resistance of Mycobacterium tuberculosis; secondly, it is difficult to achieve good therapeutic effect on tuberculosis patients infected with drug-resistant Mycobacterium tuberculosis

(2) Unsafe

The advantage of this inspection method is that the equipment is simple and the investment is small; but it has the following disadvantages: (1) the required time is long

However, its main disadvantages are that the price of instruments and equipment is high, and the investment is large; the cost of use is high. First, the use cost of instruments and equipment is high. It is difficult for hospitals to bear and popularize

[0005] As can be seen from the above detailed analysis of the existing Mycobacterium tuberculosis drug susceptibility test method, there are following shortcomings in the existing disc test method and concentration test method: (1) the required time of the whole inspection process is long

(2) troublesome operation

(3) Unsafe

(4) prone to false detection

However, the method of using instruments to identify drug sensitivity requires a large investment, high cost of use, and is difficult to popularize.

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