Mammalian-derived peptides for the treatment of microbial infection

A mammalian, antimicrobial technique for use in the field of treating microbial infections in mammals

Inactive Publication Date: 2001-01-17
THERAGEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although such modified hemoglobin-based compounds have been administered as blood substitutes, the administration of unmodified hemoglobin, heme-free subunits or fragments, or their equivalents, for the same or other therapeutic purposes has not previously been reported. synthetic peptide

Method used

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  • Mammalian-derived peptides for the treatment of microbial infection
  • Mammalian-derived peptides for the treatment of microbial infection
  • Mammalian-derived peptides for the treatment of microbial infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The antimicrobial activity of human hemoglobin was confirmed by radiation diffusion test, and the results are summarized in Table 1,

[0067] Example 1 and Figures 1-6.

Embodiment 2

[0069] The antimicrobial activity of human hemoglobin was confirmed by the plate test, and the following data were obtained. IC against Candida albicans 50 5-6μg / ml, IC for Escherichia coli 50 It is 8-10μg / ml. IC 50 Indicates the protein concentration required to kill 50% of the detected microorganisms (ie 50% reduction in colony forming units).

Embodiment 3 and 4

[0071] The results of the antimicrobial activity of the hemoglobin α chain without heme are summarized in Table 1 and Example 3, and the results of the antimicrobial activity of the hemoglobin β chain without heme are summarized in Table 1 and Example 4.

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PUM

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Abstract

The present invention provides compositions useful as antimicrobial agents which include mammalian hemoglobin, the alpha and beta chains of hemoglobin free of heme, fragments of the alpha and beta chains that result from cyanogen bromide cleavage of the alpha and beta chains, and synthetic peptides derived therefrom. The compositions exert antimicrobial activity against both bacteria and fungi that is comparable to known antimicrobial peptides from human neutrophils, cathepsin G and azurocidin. Sensitive organisms include Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria such as Staphylococcus aureus and Streptococcus faecalis, and the fungus Candida albicans. Methods for preparing the compositions also are provided.

Description

[0001] This application claims the benefit of US Provisional Application Serial No. 60 / 061,454, filed October 8, 1997 (which provisional application is hereby incorporated by reference in its entirety). field of invention [0002] The invention relates to methods of treating microbial infections in mammals (including humans and other primates); methods of killing bacteria and fungi; A method for treating substances contaminated by microorganisms with polypeptide fragments obtained by cleaving α and β chains with cyanogen bromide or synthetic fragments thereof. The invention also relates to compositions comprising such proteins, polypeptides or fragments. Background of the invention [0003] Antibacterial peptides of natural origin have a long history. In 1939, Dubos confirmed that a certain soil bacillus (later identified as brevibacillus) produced substances that could prevent pneumococcal infection in mice. Subsequently, Hotchkiss&Dubos purified two substances composed o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/50A61K38/00A61K38/42A61L2/00A61L2/18A61L12/14C07K14/805
CPCA61L2/18A61L2/0088C07K14/805A01N63/02A61K38/42Y10S530/827A61L12/14A61L2/00Y10S530/829A61P31/04A61P31/10Y02A50/30A01N63/50A01N63/10
Inventor B·F·哈弗曼B·杜比尼克
Owner THERAGEM
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