Method for evaluating antimicrobial susceptibility of drugs

A sensitive and pharmaceutical technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve problems such as low sensitivity and reproducibility, expensive experiment cost, complicated operation steps, etc.

Active Publication Date: 2012-07-11
INST OF CHEM CHINESE ACAD OF SCI
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods overcome the shortcomings of long-term cultivation in traditional methods, complex instrument requirements, low sensitivity and reproducibility, complicated operating steps, and expensive experimental costs limit the further high-throughput application of these methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating antimicrobial susceptibility of drugs
  • Method for evaluating antimicrobial susceptibility of drugs
  • Method for evaluating antimicrobial susceptibility of drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] Synthesis of compound PPP shown in embodiment 1, formula I and compound OPV shown in formula II

[0091] Add 5.4mL of toluene and 3.6mL of 2M aqueous potassium carbonate solution into a 25mL two-necked flask, and after bubbling nitrogen gas for 30min, add 269mg of 2,5-dibromo-1,4-bis(4-bromobutoxy)benzenediol, 165mg 1,4-Neopentyldiboronate and 20 mg of tetrakis(triphenylphosphine)palladium were stirred and reacted at 95°C under nitrogen for 24 hours. After cooling, chloroform and water were added, and the chloroform layer was washed with water and dried with anhydrous magnesium sulfate. Concentrate by rotary evaporation, and acetone precipitates to obtain a light yellow solid; dissolve the solid in 20 mL of dichloromethane, add 1 mL of trimethylamine, stir at room temperature for 24 h, centrifuge, collect the precipitate and dry to obtain 176 mg of polymer product. Characterization of the product: M n =3.5×10 4 ; 1 H NMR (400MHz, DMSO): δ (ppm) 6.8-7.6 (m, 6H), 3.9-4...

Embodiment 2

[0094] Embodiment 2, PPP and OPV are used for evaluating the antimicrobial susceptibility of ampicillin sodium to escherichia coli

[0095] One, the acquisition of pathogenic bacteria solution and the cultivation of pathogenic bacteria in the culture medium containing antibiotic ampicillin sodium:

[0096] 1) Obtaining of pathogenic bacteria solution:

[0097] Take Escherichia coli TOP10 strain (Beijing Biomed Science and Technology Development Co., Ltd., CC0101) and add it to 5 mL LB medium, and cultivate overnight at 37°C. Take a certain volume of bacterial liquid, centrifuge at 3500g for 5 minutes to remove the culture medium, wash with double distilled water for 3 times, and finally adjust the concentration of the bacterial liquid to OD in double distilled water 600 =1.0, the pathogen solution used was obtained.

[0098] 2) Pathogenic bacteria (Escherichia coli TOP10 strain (Beijing Bomed Technology Development Co., Ltd., CC0101)) were cultured in a medium containing the...

Embodiment 3

[0112] Embodiment 3, PPP and OPV are used for evaluating ampicillin sodium to ampicillin-resistant escherichia coli (Amp r E.coli) antimicrobial susceptibility

[0113] One, the acquisition of pathogenic bacteria solution and the cultivation of pathogenic bacteria in the culture medium containing antibiotic ampicillin sodium:

[0114] 1) Obtaining the pathogenic bacteria solution

[0115] Ampicillin-resistant Escherichia coli (Amp r E.coli, TOP10 transfection pcDNA3 ampicillin resistance plasmid) add 5mL containing 100μg.mL -1 Incubate overnight at 37°C in ampicillin-sodium LB medium. Take a certain volume of bacterial liquid, centrifuge at 3500g for 5 minutes to remove the culture medium, wash with double distilled water for 3 times, and finally adjust the concentration of the bacterial liquid to OD in double distilled water 600 = 1.0.

[0116] The pathogenic bacteria are cultured in a medium containing the drug to be tested, ampicillin sodium

[0117] Add sterilized ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
wavelengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for evaluating antimicrobial susceptibility of drugs. The method utilizes compounds represented by general formulas (I) and (II) to assist to give quantitative susceptibility data of antibiotics against pathogens. The method has the advantages of being sensitive (as low as 2*104 c f u), quick (4-5hours), accurate, visible and the like. Besides, the method is applicable to high throughput screening of antibiotics. Compared with various systems with advanced technologies, the method needs not complicated instruments and equipment, sample treatment is simple, cost is low, outside non-specific effects are few, and antibiotic concentration in inhibition percentage can be optionally provided. In clinic, the method can be used for quick evaluation of antimicrobial susceptibility to instruct doctors to perform drug administration on patients more pertinently, thereby not only treatment efficiency is improved, but also drug resistance caused by incorrect use of the antibiotics is lowered. Meanwhile, the method for evaluating antimicrobial susceptibility of drugs can also have profound effects in terms of food safety, environment detecting, and the like. Formula I and formula II are as followed.

Description

technical field [0001] The invention relates to a method for evaluating drug antibacterial susceptibility. Background technique [0002] With the extensive and irrational use of antibiotics, the drug resistance of bacteria has gradually increased, and more and more single-drug and multidrug-resistant bacteria have been reported, such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin Drug-resistant enterococci (VRE), drug-resistant strains of Mycobacterium tuberculosis (MDR-TB), and recently the Enterobacteriaceae "superbugs" carrying New Delhi metallo-β-lactamase-1 (NDM-1) have attracted widespread attention. The emergence of these drug-resistant bacteria poses a severe challenge to human public health safety, so it is particularly important to develop a fast, simple and high-throughput method for the evaluation of antimicrobial susceptibility. Among them, the minimum inhibitory concentration (MIC) as a quantitative index is widely used in the evaluation of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08G61/10G01N21/64
Inventor 王树朱春雷杨琼
Owner INST OF CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap