Recombinant adenovirus expressing mycoplasma hyopneumoniae P102 protein and applications thereof

A recombinant adenovirus, cgmccno.2987 technology, applied in application, virus/phage, recombinant DNA technology, etc., can solve the problems of inactivated vaccine and attenuated vaccine promotion and application, achieve low human pathogenicity and broad application prospects , good stability
CN101659954BInactive Publication Date: 2011-10-12HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2011-10-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a recombinant adenovirus expressing mycoplasma hyopneumoniae P102 protein and applications thereof. The recombinant adenovirus is obtained by firstly carrying out mutation on gene of the mycoplasma hyopneumoniae P102 protein, cloning the whole genome (SEQ ID No:1) after the mutation onto a shuttle vector pShuttle-CMV, obtaining a recombinant shuttle vector, carrying out electro-transformation into competent cells containing an adenovirus backbone vector after linearization and carrying out homologous recombination in bacteria. The indirect immunofluorescence and the Western Blot identification prove that the recombinant adenovirus successfully expresses the P102 protein. The recombinant adenovirus is used for immunizing female Balb / c mice with 6-8 weeks by two waysof muscle injection and nasal dropping, and the recombinant virus can induce the mice to produce the specific humoral immune and the cellular immune response, thereby indicating that the recombinant adenovirus can be applied in the diagnosis, the prevention or the treatment of mycoplasma pneumoniae of swine.
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Description

technical field

[0001] The present invention relates to a rearranged cDNA sequence and a recombinant adenovirus containing the cDNA sequence, in particular to a cDNA sequence obtained by rearranging and optimizing the gene encoding the Mycoplasma hyopneumoniae P102 protein, and the present invention also relates to a recombinant adenovirus containing the cDNA The sequenced recombinant adenovirus and their application in the diagnosis, prevention or treatment of swine mycoplasma pneumonia belong to the field of prevention and treatment of swine mycoplasma pneumonia. Background technique

[0002] Mycoplasma hyopneumoniae (Mycoplasma, hyopneumoniae, Mhp) is the main pathogen of swine mycoplasma pneumonia (MPS), which is also called porcine endemic pneumonia (EPP). . MPS is characterized by contact, highly contagious, chronic, high morbidity, and low mortality. The main manifestations are cough, dyspnea, and fleshy or marbled lesions in the lung tissue can be seen on dissection...

Claims

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