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Metarhizium flavoviride MFYY090714 and applications thereof

A technology of MFYY090714, Metarhizium anisopliae chrysanthemum, applied in application, fungi, biocide, etc., to achieve the effects of rapid growth, high spore germination rate, and large spore production

Inactive Publication Date: 2014-10-29
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Metarhizium anisopliae has good genetic stability, and no toxicity has been found to humans, livestock, poultry, and plants. There has been no report on the use of this fungus in the control of white-backed planthoppers

Method used

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  • Metarhizium flavoviride MFYY090714 and applications thereof
  • Metarhizium flavoviride MFYY090714 and applications thereof
  • Metarhizium flavoviride MFYY090714 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment one, the isolation of pathogenic bacteria, identification

[0010] 1. Materials and methods

[0011] 1.1 Materials

[0012] Adults of the diseased white-backed planthopper Sogatella furcifera (Horváth) infected by an entomogenic fungus were collected from terraced paddy fields in Yuanyang, Yunnan Province.

[0013] Potato dextrose (PDA) medium: 200g of peeled potatoes, cut into small pieces, add 1L of water, filter with gauze after 20min, add 20g of glucose and 15-20g of agar to the filtrate, heat to dissolve, and set to 1000mL Aseptic operating conditions: all All the utensils and utensils were sterilized in a high-temperature autoclave (121°C, 30min), and the inoculation and other operations were all carried out in an ultra-clean workbench.

[0014] Culture conditions: Culture in a 26°C light (12L:12D) incubator. After the colonies are formed, transfer to the test tube PDA slope, cultivate for 3 to 4 days, and transfer to a 4°C refrigerator for storage. ...

Embodiment 2

[0025] Embodiment two, biological characteristics of metarhizium anisopliae purified strain

[0026] 1. Materials and methods

[0027] 1.1 The strains to be tested Select a plate that grows uniformly and vigorously after purification as the strains to be tested. The hyphae were inoculated on PDA medium again, and cultivated in a constant temperature light (12L:12D) incubator at 26°C.

[0028] 1.2 Determination of colony growth rate and spore production. Take a plate of Metarhizium anisopliae that has been cultured in advance and use an 8mm puncher to punch a hole to get the bacteria, inoculate it on a new medium, do three repetitions, and place it at 26°C Cultured in a constant temperature and light (12D:12L) incubator, the diameter was regularly measured and recorded every day until the colony was full of the culture medium. Use a hole puncher with a diameter of 8mm to take the bacterium cake at the same position of the culture medium, add 1% Tween-80 and 20ml sterile water...

Embodiment 3

[0039] Example 3. Indoor pathogenicity determination of purified strains of Metarhizium anisopliae to white-backed planthopper

[0040] 1. Materials and methods

[0041]1.1 Source of tested insects

[0042] Select white-backed planthopper adults of the same batch, disease-free, and of the same size 24 hours after eclosion as test insects, and place the tested white-backed planthopper adults on rice seedlings planted in insect cages at 26±1°C and 12L: reared under the photoperiod conditions of 12D.

[0043] 1.2 Preparation of spore suspension

[0044] Get the purifying Metarhizium anisopliae that grow well and wash the conidia with sterile water and filter to prepare 10 8 Spores / ml conidia suspension, use a sterile capillary pipette to take a drop of the filtrate onto a hemocytometer, count the spores under a microscope and record the data, and dilute to 10 with sterile water containing 0.5% Tween 80 8 、10 7 、10 6 、10 5 、10 4 The spore suspensions with five concentratio...

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Abstract

The invention provides a Metarhizium flavoviride MFYY090714 and applications thereof. The Metarhizium flavoviride MFYY090714 is preserved in China General Microbiological Culture Collection Center on April 8th, 2014. The preservation number is CGMCC No.9020. The bacterium strain is a novel bacterium strain separated from the body of sogatella furcifera. The culture is simple, the growth is rapid, the spore yield is high, and the germination rate of the spores is high. The pathogenicity of the Metarhizium flavoviride spore suspension liquid on sogatella furcifera is high, moreover, the Metarhizium flavoviride spore suspension liquid is environment-friendly and pollution-free, does not generate any drug resistance, and can be widely used to control sogatella furcifera in practice.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to Metarhizium anisopliae MFYY090714 and its application. Background technique [0002] The white-backed planthopper is an important pest of rice, often referred to as the rice planthopper together with the brown planthopper and the brown planthopper. In recent years, white-backed planthopper has become the dominant species of rice planthopper in southwestern China, especially in Yunnan rice-growing area. Due to its characteristics of fast reproduction, migration, and strong drug resistance, it can cause severe rice yield reduction and even The crop failure seriously restricts the production of rice. The control of white-backed planthopper has always been an important content of pest control research, but for a long time it has mainly relied on chemical pesticide control, and the extensive use of chemical pesticides has threatened human health and the envir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 陈斌杜广祖肖关丽和淑琪张立敏桂富荣李正跃严乃胜
Owner YUNNAN AGRICULTURAL UNIVERSITY
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