Isaria javanicanicus mould strain and application thereof in preventing and controlling prodenia litura

A technology of Spodoptera litura and Corynebacteria, applied in the directions of application, fungi, botanical equipment and methods, etc.

Inactive Publication Date: 2009-08-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Isaria originally belonged to the genus Paecilomyces. It is an important class of insect pathogens and is widely distributed. It can be used to control nemato...

Method used

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  • Isaria javanicanicus mould strain and application thereof in preventing and controlling prodenia litura
  • Isaria javanicanicus mould strain and application thereof in preventing and controlling prodenia litura
  • Isaria javanicanicus mould strain and application thereof in preventing and controlling prodenia litura

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A highly effective and effective strain against Spodoptera litura was obtained by collecting the carcasses of a susceptible Spodoptera litura in Guangzhou, using conventional general techniques to isolate and purify strains, determining pathogenicity, comparing biological characteristics and production traits, and determining production strains. Isaria javanicus (Friedrichs & Bally) strain Ij01 with good stability.

[0022] Preparation of spore suspension: pick spores from the slant bacteria, insert them into 0.05% Tween-80 solution that has been sterilized, measure the spore concentration with a hemocytometer, and adjust to 10 7 Spores / mL.

[0023] Seed culture: inoculate 2 mL of spore suspension into 98 mL of seed medium, shake the flask at 180 r / min and 26 ° C for 2 to 3 days to form a seed liquid.

[0024] Seed medium composition: Czapek-Dox with 0.5% peptone: Peptone 5g, NaNO 3 3g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, sucrose ...

Embodiment 2

[0028] Embodiment 2 utilizes this bacterial strain to control Spodoptera litura

[0029] With reference to the conidia that the method described in Example 1 harvests, be formulated with Tween-80 solution and be that concentration is 10 6 ~10 7 Suspension of conidia / mL, the suspension is applied to the field by foliar spraying method, and it is applied at the peak of the second instar larvae of Spodoptera litura.

[0030] Toxicity of the Ij01 strain to Spodoptera litura: Under different spore concentrations, the average cumulative mortality of the 2nd instar larvae of Spodoptera litura increased significantly with the increase of treatment concentration and the extension of time, see Table 1, when the concentration was 50000 When conidia / mL, the mortality rate of Spodoptera litura reached 48.33% and 51.26% respectively on the 6th and 8th day after treatment.

[0031] The half-lethal dose was calculated by the logarithmic-probability value analysis method of the observed data...

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Abstract

The invention discloses an Isaria javanicanicus mould strain and application thereof. The high-efficiency Isaria javanicanicus (Friedrichs & Bally) strain Ij01 is classified to belong to Corynespora, Hyphomycetaceae, Hyphomycetales, Hyphomycetes, Mitosporic Fungi, Eumycota. The strain has been preserved in China Center for Type Culture Collection (CCTCC), the preservation date is Oct 27, 2008, and the preservation number is CCTCC M 208171. The high-efficiency Isaria javanicanicus mould is entomopathogenic fungi to have high efficiency for preventing and controlling prodenia litura, has the characteristics of brand-new action mechanism different from the prior chemical insecticides, and no environmental pollution and residue when taken as a living biological pesticide, and meets the requirement of organic food production.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to high-efficiency I. javanica strains and their application in pest control. Background technique [0002] Spodoptera litura (Fabricius) is a worldwide pest of high concern. Its feeding habits are extremely mixed, and it can eat more than 290 kinds of crops in 99 families such as Cruciferae, Solanaceae, and Cucurbitaceae. Spodoptera litura was originally a pest that occurred intermittently, and it occurred once in about 3 to 4 years. After the 1990s, due to the adjustment of agricultural planting structure, the area of ​​economic crops such as vegetables and the area of ​​facility cultivation increased rapidly, increasing the number of twill The feeding, breeding and overwintering places of noctuid moths, as well as the high temperature and less rain in summer and the excessive use of pesticides to kill natural enemies, have caused spodoptera litura disasters to ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 任顺祥胡琼波
Owner SOUTH CHINA AGRI UNIV
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