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Endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and application thereof to biocontrol

A technology of Serratia and ZF05, which is applied in agricultural biological control, from the endophytic antagonistic bacteria of Fenghuang Dancong tea tree and its application in biological control, can solve the problems that there are not many researches on Fenghuang Dancong tea, and achieve strong Inhibition effect, effect of good application prospect

Inactive Publication Date: 2016-09-28
HANSHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Fenghuang Dancong tea mainly focuses on the variety identification, genetic diversity analysis, quality chemical characteristics, physiological activity, quality safety and greening process. There are not many studies on the endophytic bacteria of Fenghuang Dancong tea tree.

Method used

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  • Endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and application thereof to biocontrol
  • Endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and application thereof to biocontrol
  • Endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and application thereof to biocontrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation, screening and identification of Serratia sp. ZF05GDMCC No: 60015

[0038] 1. Isolation and screening of strains

[0039] The Serratia sp. used in the present invention is sampled from the leaves of Phoenix Dancong tea leaves in Fubin Town, Raoping County, Chaozhou, Guangdong Province by Hanshan Teachers College. In Fubin Town, Raoping County, Chaozhou, the trees are 15, 25 and 50 years old, and the planting altitude is 750m. The tea tree variety is Fenghuang Dancong tea narcissus, which is organically planted. After using the homogenate coating method to isolate the endophytic bacteria in the leaves, using different culture temperatures, pH values, and medium as the enrichment conditions, a batch of well-growing bacterial strains were screened out through optimization, and one strain numbered as Strains of ZF05.

[0040] Separation steps:

[0041] (1) Separation medium adopts: beef extract peptone liquid medium is beef extract 5g, peptone 10g, s...

Embodiment 2

[0056] Example 2: Molecular level identification of Serratia sp. ZF05GDMCC No: 60015

[0057] The bacterial DNA genome rapid extraction kit was used to extract the DNA of the target strain.

[0058] The primers are bacterial universal primers, bacterial 16SrDNA universal primer sequence:

[0059] 27F: 5′-AGAGTTTGATCCTGGCTCAG-3′

[0060] 1492R: 5'-GGTTACCTTGTTACGACTT-3'

[0061] Primer 27F, primer 1492R, and 2×TapPCR Master mix used in this application were all purchased from Biological Engineering (Shanghai) Co., Ltd.

[0062] The 20 μL PCR amplification system used to extract genomic DNA: 1.0 μL each of primer 27F and primer 1492R, 7.0 μL of 2×TapPCCR Master mix, 1.0 μL of endophytic bacterial DNA mixture, and 10.0 μL of double distilled water.

[0063] PCR amplification program: pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 30s, annealing at 55°C for 30s, extension at 72°C for 40s, 30 cycles from denaturation to extension, extension at 72°C for 7 minutes,...

Embodiment 3

[0067] Example 3: Growth Factors of Serratia sp. ZF05GDMCC No: 60015

[0068] Table 2: Effects of temperature, pH and NaCl on the growth of strain ZF05

[0069] temperature(℃)

4

10

15

20

25

30

37

40

growing situation

+

+

++

++

+++

++++

+

pH

4

5

6

7

8

9

growing situation

-

+

++

+++

+

-

NaCl concentration

6％

5％

4％

3％

2％

1％

growing situation

-

+

+

+

++

+++

[0070] Serratia sp. (Serratia sp.) ZF05GDMCC No: 60015 is cultivated according to the above method. The culture conditions of the bacteria are as follows: the culture medium is beef extract peptone liquid medium and nutrient agar medium, and the culture conditions: pH7.0, temperature Cultivate for 20 h at 37°C.

[0071] It can be concluded from Table 2 that strain ZF05 is the most suit...

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Abstract

The invention discloses endophytic antagonistic Serratia sp. ZF05 from Fenghuang Dancong tea plants and an application thereof to biocontrol. Sampling from Fenghuang Dancong tea plant leaves, separation and purification, screening and cultivation are carried out in order to obtain a strain of Serratia sp. ZF05 GDMCC No:60015 with good biocontrol effects. The bacterial strain is obviously different from common Serratia sp. in the aspects of physiological-biochemical characteristics and molecular level, so the bacterial strain is a typical new bacterial strain. The bacterial strain ZF05 has good inhibition effects for Alternaria alternate, Walnut blight, Alternaria sp., Rhizoctonia solani, and Pythium aphanidermatum; mycelium growth inhibition rates are as follows: 73.02%, 73.70%, 78.51%, 84.56% and 20.06%; Serratia sp. ZF05 is a biocontrol bacterium with obvious typicality and high specificity, and has good application prospects for developing new biopesticides or biological control microbial inoculum; Serratia sp. ZF05 has wide application values for biocontrol.

Description

technical field [0001] The invention relates to the technical field of agricultural biological biocontrol, in particular to the technical field of an endophytic antagonistic bacteria from Fenghuangdancong tea tree and its application in biological control. Background technique [0002] Endophyte refers to a class of microorganisms that survive in various tissues or intercellular spaces of healthy plants during part or all of their life history, without causing obvious pathological symptoms to the host. Antagonistic endophytes refer to microorganisms capable of producing antagonistic effects. Plants are a complex micro-ecological system. Healthy plants contain a large number of endophytic bacteria (Endophytic Bacteria), which have been isolated and identified from more than 50 kinds of plants such as wheat, cotton, rice, peanut, potato, tomato, lemon, and citrus. There are more than 50 genera of endophytic bacteria, and almost all healthy plants contain endophytic bacteria. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/425
CPCA01N63/00C12N1/205C12R2001/425
Inventor 傅力蔡丽欧燕清章斌王忠合
Owner HANSHAN NORMAL UNIV
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