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Cunninghamia lanceolata endophytic fungus and biological prevention and treatment application thereof

A technology of endophytic fungi and biological control preparations, which can be used in applications, microorganisms, fungi, etc., and can solve the problems of environmental pollution, human and animal health risks, bacteria resistance, etc.

Active Publication Date: 2020-10-20
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control of Chinese fir anthracnose mainly relies on chemical control, which may easily lead to problems such as environmental pollution, human and animal health risks, and drug resistance of bacteria

Method used

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  • Cunninghamia lanceolata endophytic fungus and biological prevention and treatment application thereof
  • Cunninghamia lanceolata endophytic fungus and biological prevention and treatment application thereof
  • Cunninghamia lanceolata endophytic fungus and biological prevention and treatment application thereof

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Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: Chinese fir endophyte Epicoccum dendrobii SMEL isolation and identification

[0026] In this experiment, healthy Chinese fir branches were collected in Nanjing Forestry University in April 2017 (collector: Huang Lin;

[0027] Tel: 025-85427301), wash with sterile water, and then sterilize the surface with 75% absolute ethanol, remove the epidermis, transfer the internal tissue to a PDA plate, culture at 28°C, and use sterile picking after 7 days The edge part of the colony was picked with a needle and inoculated on a new PDA plate to purify the strain to obtain the endophytic fungus strain SMEL of Chinese fir. The colony of this strain on PDA medium has neat edges, and the aerial mycelium is felt-like to flocculent, flat, white to light brown; the back of the colony is light red, and the center is light brown to brown. The hyphae have a septum, and the width of the hyphae is 1.4-4.1 μm; the subsealets are black, superficial or partially buried in the m...

Embodiment 2

[0029] Embodiment 2: SMEL inhibits the growth of Chinese fir anthracnose

[0030] The SMCG1#C strain of Chinese fir anthracnose was provided by the Forest Pathology Laboratory of Nanjing Forestry University. The strain was isolated from the leaves of Chinese fir infected with anthracnose. Observation. Nanjing Forestry University Journal of Nanjing Forestry University (Natural Science Edition), 41(6):68-72. Open. Inoculate C. fir anthracnose and SMEL onto PDA plates, culture them at 25°C for 5 days and use a sterile puncher to prepare bacteria blocks with a diameter of 5 mm; Simultaneously, the SMEL bacteria blocks were inoculated at positions 30 mm away from the fir anthracnose bacteria cake in the vertical direction, and only the pathogenic fungus cake was inoculated on the control plate. The inoculated plate was cultured in a 25 °C incubator for 5 days, and the antibacterial effect of SMEL on Anthracnose fir was determined. Experiments were repeated three times, with three...

Embodiment 3

[0031] Embodiment 3: SMEL is to the inhibitory action of several kinds of plant pathogenic fungus mycelia growth

[0032] Plant pathogenic bacteria blocks were inoculated in the center of the PDA plate (preparation process is the same as above), and SMEL bacterial blocks (same as above) were inoculated in four vertical directions 30 mm away from the bacterial cakes, and the control plate was only inoculated with pathogenic fungal cakes. The inoculated plates were cultured in a 25°C incubator for 3-5 days, and the antibacterial effect of SMEL on the other five plant pathogenic bacteria was determined. Experiments were repeated three times, with three replicates for each treatment. The result is as Figure 4 , it can be seen from the figure that SMEL has obvious inhibitory effect on the five pathogenic bacteria.

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Abstract

The invention relates to a cunninghamia lanceolata endophytic fungus and a biological prevention and treatment application thereof. The cunninghamia lanceolata endophytic fungus is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M 2019895, and the preservation date is November 4, 2019. The Epicoccum dendrobii SMEL of the invention is the cunninghamialanceolata endophytic fungus, and can inhibit the growth of Cunninghamia lanceolata colletotrichum and reduce the biomass accumulation amount of Cunninghamia lanceolata colletotrichum; the inoculation test result shows that the plaque development of cunninghamia lanceolata anthracnose is significantly inhibited after inoculation with SMEL bacteria; and the SMEL strain has certain antagonistic effects against Cunninghamia lanceolata colletotrichum, Colletotrichum gossypii, Rhizoctonia sp., Cytospora chrysosperm, Fusicoccum aesculi and Alternaria sp. on the plate.

Description

technical field [0001] The invention belongs to the field of microbial pesticides, in particular to an endophytic fungus of Chinese fir and its biological control application. Background technique [0002] Chinese fir( Cunninghamia lanceolata ) is widely cultivated in the Yangtze River Basin and south of the Qinling Mountains in my country, and is one of the most important fast-growing timber tree species in my country due to its advantages of fast growth, straight and straight stem shape, straight wood texture, insect resistance and corrosion resistance, and high economic value. . my country's existing Chinese fir forest is 7.541 million ha 2 , with a storage capacity of 263.7 million m 3 , of which the plantation area is 4.498 million hm 2 , accumulating 149.8 million m 3 , the area of ​​artificial Chinese fir forest accounts for 24.0% of the total area of ​​the national plantation forest, and the stock of artificial Chinese fir forest accounts for 28.3% of the nationa...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 黄麟卞金月方玉兰宋青孙美玲巨云为
Owner NANJING FORESTRY UNIV
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