Sea-buckthorn endophytic fungi, preparation method of its extract product and application thereof

A technology of endophytic fungi and extracts, applied in the field of microorganisms, can solve problems such as damage, and achieve the effects of improving drought resistance, less dosage, and good drought tolerance

Active Publication Date: 2015-09-16
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, using phytochemical regulation measures to improve turfgrass growth and enhance resistance is a relatively active field in turfgrass research. Due to the variety of growth regulators, the use of There are also differences, a little carelessness in the process of application can easily cause unnecessary damage

Method used

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  • Sea-buckthorn endophytic fungi, preparation method of its extract product and application thereof
  • Sea-buckthorn endophytic fungi, preparation method of its extract product and application thereof
  • Sea-buckthorn endophytic fungi, preparation method of its extract product and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A strain CBS1 that can induce turfgrass to improve drought resistance was isolated from endophytic fungal samples collected from wild seabuckthorn. The strain grows fast and can improve the drought resistance of turfgrass under simulated drought conditions. According to its morphological and genetic analysis, the CBS1 strain was identified as Alternaria ( Alternaria Sp ), the deposit number is CGMCC NO.10117, the deposit date is 2014.12.11, the name of the depository unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures; the address of the depository unit: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0032] The production technology of seabuckthorn endophytic mycelia extract of the present invention comprises the following steps:

[0033] Step 1. Isolation of seabuckthorn endophytic fungi: ①Process and wash healthy wild seabuckthorn samples immediately after collection, then cut seabuckthorn roots into sma...

Embodiment 2

[0041] The fungus isolated in the step 2 is connected to the plate PDA medium, cultivated at 20°C for 8 days; cultivated at 25°C, 150r / min on a rotary shaker for 4 days as seeds; at 25°C, rotated at 150r / min Cultivate on the bed for 5 days and stop the fermentation.

[0042] In step 3, the mycelium is washed and dried at 50° C., extracted for 28 hours, extracted 4 times, and ultrasonically oscillated for 0.8 hours. Other steps are the same as in Example 1.

Embodiment 3

[0044] The fungus isolated in the step 2 is connected to the flat PDA medium, cultured at 28°C for 4 days; cultivated at 30°C, 100r / min on a rotary shaker for 2 days as seeds; at 30°C, rotated at 100r / min The bed was cultivated for 2 days, and the fermentation was terminated.

[0045] In step 3, the mycelium is washed and dried at 70° C., extracted for 20 hours, extracted twice, and ultrasonically oscillated for 1.5 hours. Other steps are the same as in Example 1.

[0046]Taking perennial bluegrass as the experimental material, the changes of morphological and physiological and biochemical indicators of turfgrass sprayed with alcohol extract of endophytic fungus CBS1 under 4 concentrations of PEG-6000 simulated drought stress were studied. The results showed that under the stress of different concentrations of PEG-6000, CBS1 could alleviate the effect of drought stress to a certain extent, and the effect of reducing the effect was the most significant when spraying 20% ​​PEG-...

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Abstract

The invention relates to a strain of fungi in wild sea-buckthorn root from Changbai Mountain and an application of the fungi for increasing turfgrass drought tolerance. The sea-buckthorn endophytic fungus is Alternaria Sp, a preservation number is CGMCC No. 10117, the preservation date is 11th, Dec, 2014, and the endophytic fungus is preserved by China General Microbiological Culture Collection Center. The concentration of a mycelia extract product is nanogram level, so that the endophytic fungi extract product has high activity, and the endophytic fungus can induce the turfgrass to obtain good drought tolerance by spraying the turfgrass with 0.5ng / ml concentration.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a fungus derived from the root of wild seabuckthorn in Changbai Mountain and its application in improving the drought tolerance of turfgrass. Background technique [0002] With the acceleration of urban ecological construction, people's requirements for urban green space construction are also increasing. As an excellent greening species, turfgrass has been widely used in urban green space construction. However, due to my country's vast territory and complex geographical and climatic conditions, the same turfgrass often has completely different growth performances in different regions. Turfgrass plants show a certain limit of tolerance in adverse environments such as cold, high temperature, drought, waterlogging and saline-alkali. However, when the adversity stress exceeds the tolerance range of turfgrass, the enzymes in turfgrass are destroyed, and the normal metab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P1/02A01N63/02A01P21/00C12R1/645
CPCA01N63/30C12P1/02C12N1/145C12R2001/645
Inventor 肇莹肖军马晓颖王红陈珣杨涛杨镇王岩任志莹
Owner LIAONING ACAD OF AGRI SCI
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