Bacillus amyloliquefaciens strain and application thereof in preventing and controlling powdery mildew of tobacco

A technology for desolvating amyloliquefaciens and bacterial strains, applied in the field of microorganisms, can solve problems such as Bacillus amyloliquefaciens that have not yet been found, and achieve the effects of broad bacteriostatic spectrum, strong bacteriostatic activity, and increased yield

Inactive Publication Date: 2016-07-06
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bacillus amyloliquefaciens that can inhibit and prevent tobacco powdery mildew has not been found yet. A microbial bacterial agent developed by the present invention can achieve effective control of tobacco powdery mildew

Method used

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  • Bacillus amyloliquefaciens strain and application thereof in preventing and controlling powdery mildew of tobacco
  • Bacillus amyloliquefaciens strain and application thereof in preventing and controlling powdery mildew of tobacco
  • Bacillus amyloliquefaciens strain and application thereof in preventing and controlling powdery mildew of tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1: the culture method of D1 bacterial strain

[0062] (1) Preparation of Mabi wood plant tissue: get the root of fresh Mabi wood, rinse it under tap water;

[0063] (2) Surface disinfection and sterility testing: On an ultra-clean workbench, wash the material twice with 1‰ Tween-80 sterile water, then wash with sterile water until there is no foam, and absorb the surface with sterile filter paper Moisture; Soak in 75% alcohol for 4 minutes, rinse with sterile water for 3 times; 0.1% mercury liter surface disinfection for 1 minute, and then rinse with sterile water for 3 times, collect the last rinse water for microbial detection, aseptic only, sterile Filter paper to absorb excess water; cut or shear the material into tissue sections with a diameter of 4mm;

[0064] (3) Separation and purification of endophytic bacteria: inoculate the tissue sections after disinfection in step (2) on a PDA medium plate, and cultivate them at a constant temperature of 22°C fo...

Embodiment 2

[0065] Embodiment 2: the culture method of D1 bacterial strain

[0066] (1) Preparation of Mabi wood plant tissue: get the root of fresh Mabi wood, rinse it under tap water;

[0067] (2) Surface disinfection and sterility testing: On an ultra-clean workbench, wash the material 3 times with 3‰ Tween-80 sterile water, then wash with sterile water until there is no foam, and absorb the surface with sterile filter paper Moisture: Soak in 75% alcohol for 2 minutes, rinse twice with sterile water; disinfect the surface with 0.3% mercuric chloride for 3 minutes, then rinse with sterile water for 4 times, collect the last rinse water for microbial testing, sterile only, sterile Filter paper to absorb excess water; cut or shear the material into tissue sections with a diameter of 6mm;

[0068] (3) Separation and purification of endophytic bacteria: inoculate the tissue sections after disinfection in step (2) on a PDA medium plate, and cultivate them at a constant temperature of 28°C f...

Embodiment 3

[0069] Embodiment 3: the culture method of D1 bacterial strain

[0070] (1) Preparation of Mabi wood plant tissue: get the root of fresh Mabi wood, rinse it under tap water;

[0071] (2) Surface disinfection and sterility testing: on an ultra-clean workbench, wash the material 3 times with 5‰ Tween-80 sterile water, then wash with sterile water until there is no foam, and absorb the surface with sterile filter paper Moisture: Soak in 75% alcohol for 3 minutes, rinse with sterile water for 3 times; disinfect the surface with 0.5% mercury liter for 3 minutes, then rinse with sterile water for 4 times, collect the last rinse water for microbial detection, aseptic, sterile Filter paper to absorb excess water; cut or shear the material into tissue sections with a diameter of 6mm;

[0072] (3) Separation and purification of endophytic bacteria: inoculate the tissue sections after disinfection in step (2) on a PDA medium plate, and cultivate them at a constant temperature of 30°C fo...

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Abstract

The invention discloses an antagonistic strain separated and screened from a medicinal plant nothapodytes pittosporoides. According to microbial taxonomy identification, the strain is named as a bacillus amyloliquefaciens D1 strain, and a microbial agent capable of preventing and controlling powdery mildew of tobacco is prepared from the fermentation liquid of the strain. The remarkable feature of the invention is providing the new bacillus amyloliquefaciens D1 strain, the microbial agent prepared from the fermentation liquid realizes a good prevention and control effect on the powdery mildew of tobacco, and the strain is an important microbial resource in the plant growing industry.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the application of a strain of Bacillus amyloliquefaciens and the microbial agent prepared therefrom in the prevention and treatment of tobacco powdery mildew. Background technique [0002] Tobacco (Nicotianatabacum) belongs to the family Solanaceae and is an annual herb. At present, the national tobacco planting area has reached 1 million hectares 2 -1.2 million hm 2 , The output of tobacco leaves is 1.6-2.2 million tons, ranking first in the world. Tobacco powdery mildew (Erysiphecichoracearum) is a common disease, commonly known as "upper ash" and "lower frost", and develops from the bottom of the plant upwards. The incidence rate of plants is often 100%, and the incidence rate of leaves is generally 10%-20%. It can be serious Up to 60% or more. The main symptoms are as follows: small white powdery spots appear at the beginning of the infection, and the spots expand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/02C12N1/20C12N1/205C12R2001/07
Inventor 雷帮星张涛文晓鹏乔光康冀川钱一鑫
Owner GUIZHOU UNIV
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