Application of Trichothecium roseum TR-1 and fermented product to prevention and treatment of wheat powdery mildew
A technology of trichothecene pink and powdery mildew of wheat, which is applied in the field of microorganisms to achieve the effect of high antagonistic activity and high biocontrol activity
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Embodiment 1
[0021] Embodiment 1: Isolation and identification of Trichothecene pink TR-1 of the present invention
[0022] Rinse the tissues of various parts of the collected fresh plants under running water to remove the sediment attached to the surface, and store them in a refrigerator at 4°C. The separation of endophytic fungi adopts the conventional tissue separation method. The sample is first washed twice with sterile water, cut into 1-2 cm long pieces and soaked in 75% alcohol for 1-2 minutes (the length of time is determined according to the separation site). Rinse with sterile water for 2 to 3 times. Disinfect with sodium hypochlorite with 1.5% available chlorine content for 7 to 10 minutes, rinse with sterile water for 3 times, cut the surface sterilized tissue in a sterilized petri dish with a sterilized blade For 0.2cm×0.2cm fragments, use sterilized filter paper to fully absorb the moisture on the surface of the sterilized tissue block. Finally, the tissue pieces were tran...
Embodiment 2
[0023] Embodiment 2: the different filtrates of TR-1 bacterial strain according to the present invention are to the activity of wheat powdery mildew
[0024] Using the isolated leaf section method, the wheat cultivated to 1 leaf and 1 heart, the first leaf was cut into 30mm long leaf section, placed on 0.5% water agar plate containing 50ug / ml benzimidazole, each plate (diameter 60mm ) to place 8 leaf segments. Add surfactant Tween 20 (final concentration is 2.5 × 10 -4 ) and the fermented liquid are fully mixed, applied in the spray tower, and the spray volume is 4ml. Set up 6 treatments, respectively 4ml, 3ml, 2ml, 1ml, 0.5ml and 0ml of the TR-1 strain filtrate, and make up the remaining 4ml with sterile water. Simultaneous spraying containing 2.5×10 -4 The PDB solution of Tween 20 was used as the negative control, and the water was sprayed as the blank control. After 24 hours, it was inoculated with conidia of powdery mildew of wheat (15 conidia / 10 times visual field). ...
Embodiment 3
[0027] Embodiment 3: described in the present invention TR-1 Determination of the Activity of Fermentation Broth Extracts of Strains on Wheat Powdery Mildew
[0028] After the fermentation was terminated, the fermentation product was filtered, the filtrate was centrifuged at 11000r / min for 10min, the supernatant was taken, and the active fermentation broth was freeze-dried, and the obtained dry powder was sequentially extracted with equal volumes of ether and ethyl acetate for 5 times, each time for 10min. The organic phase was collected, concentrated by rotary evaporation to obtain an extract for later use. Aqueous phase recovery. Take the extract of the fermentation product of LPS-1 strain, make it into 10mg / L, and carry out activity determination. Using the isolated leaf method, the spray volume is 350mL / m 2 , dried naturally, inoculated with fresh wheat powdery mildew bacteria after 24 hours, and solvent treatment was set as negative control and blank control. Place...
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Abstract
Description
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Application Information
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