Application of Trichothecium roseum TR-1 and fermented product to prevention and treatment of wheat powdery mildew

A technology of trichothecene pink and powdery mildew of wheat, which is applied in the field of microorganisms to achieve the effect of high antagonistic activity and high biocontrol activity

Inactive Publication Date: 2016-01-06
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports at home and abroad about the activity of this fungus against wheat powdery mildew

Method used

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  • Application of Trichothecium roseum TR-1 and fermented product to prevention and treatment of wheat powdery mildew
  • Application of Trichothecium roseum TR-1 and fermented product to prevention and treatment of wheat powdery mildew
  • Application of Trichothecium roseum TR-1 and fermented product to prevention and treatment of wheat powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Isolation and identification of Trichothecene pink TR-1 of the present invention

[0022] Rinse the tissues of various parts of the collected fresh plants under running water to remove the sediment attached to the surface, and store them in a refrigerator at 4°C. The separation of endophytic fungi adopts the conventional tissue separation method. The sample is first washed twice with sterile water, cut into 1-2 cm long pieces and soaked in 75% alcohol for 1-2 minutes (the length of time is determined according to the separation site). Rinse with sterile water for 2 to 3 times. Disinfect with sodium hypochlorite with 1.5% available chlorine content for 7 to 10 minutes, rinse with sterile water for 3 times, cut the surface sterilized tissue in a sterilized petri dish with a sterilized blade For 0.2cm×0.2cm fragments, use sterilized filter paper to fully absorb the moisture on the surface of the sterilized tissue block. Finally, the tissue pieces were tran...

Embodiment 2

[0023] Embodiment 2: the different filtrates of TR-1 bacterial strain according to the present invention are to the activity of wheat powdery mildew

[0024] Using the isolated leaf section method, the wheat cultivated to 1 leaf and 1 heart, the first leaf was cut into 30mm long leaf section, placed on 0.5% water agar plate containing 50ug / ml benzimidazole, each plate (diameter 60mm ) to place 8 leaf segments. Add surfactant Tween 20 (final concentration is 2.5 × 10 -4 ) and the fermented liquid are fully mixed, applied in the spray tower, and the spray volume is 4ml. Set up 6 treatments, respectively 4ml, 3ml, 2ml, 1ml, 0.5ml and 0ml of the TR-1 strain filtrate, and make up the remaining 4ml with sterile water. Simultaneous spraying containing 2.5×10 -4 The PDB solution of Tween 20 was used as the negative control, and the water was sprayed as the blank control. After 24 hours, it was inoculated with conidia of powdery mildew of wheat (15 conidia / 10 times visual field). ...

Embodiment 3

[0027] Embodiment 3: described in the present invention TR-1 Determination of the Activity of Fermentation Broth Extracts of Strains on Wheat Powdery Mildew

[0028] After the fermentation was terminated, the fermentation product was filtered, the filtrate was centrifuged at 11000r / min for 10min, the supernatant was taken, and the active fermentation broth was freeze-dried, and the obtained dry powder was sequentially extracted with equal volumes of ether and ethyl acetate for 5 times, each time for 10min. The organic phase was collected, concentrated by rotary evaporation to obtain an extract for later use. Aqueous phase recovery. Take the extract of the fermentation product of LPS-1 strain, make it into 10mg / L, and carry out activity determination. Using the isolated leaf method, the spray volume is 350mL / m 2 , dried naturally, inoculated with fresh wheat powdery mildew bacteria after 24 hours, and solvent treatment was set as negative control and blank control. Place...

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Abstract

The invention relates to the technical field of the microbial technology, an entophytic fungus is separated from strawberries and named Trichothecium roseum TR-1 through microbial taxonomy identification, and the strain is preserved in CCTCC (China center for type culture collection) (address: Wuhan University, Wuhan, China) with the preservation number of CCTCC NO:M 2015344. The Trichothecium roseum TR-1 is significantly characterized in that a strain liquid fermentation broth and an ethyl ether extract have the better inhabitation effect on the wheat powdery mildew. The excellent original strain is provided for further research and development of the Trichothecium roseum, and the strain is an important microbial resource in agriculture.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to the application of endophytic fungi in plants, in particular to endophytic fungi in plants Trichothecium roseum Application of TR-1 or its fermented product in controlling wheat powdery mildew. Background technique [0002] Trichothecenes pink ( Trichothecium roseum link ex Fries) belongs to the phylum Fungi, half-knowledge fungi, the order Pyrichospora, the family Pyremyceae, the subfamily Gemini, and the genus Trichothecenes. T. roseum It is closely related to people's production and life, mainly in several aspects: (1) the bacterium can cause various crop diseases, such as papaya fruit rot, currant pink powder disease, chestnut solid rot, banana and grape red powder disease, cotton powdery mildew, apple moldy core, bagged pear black spot, tomato pink powdery and corn pink polythecene ear rot, etc.; (2) the secondary metabolite produced by the fungus - pink trichothec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00
Inventor 喻大昭龚双军向礼波杨立军
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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