The invention relates to a rapid freehand sectioning method for main vein supporting leaves. The rapid freehand sectioning method comprises the steps of putting collected mature, healthy and complete leaves with no pests and diseases into clear water, washing, soaking leaf stalks into the clean water, taking out leaves, trimming along two sides of main veins until leaves with the widths of 2mm-6mm along two sides of the main veins and the main veins with the lengths of 2cm-6cm are preserved, averagely cutting leaves into two sections, putting two sections of epidermises containing the main veins of the leaves face to face, holding the material by virtue of an index finger and a thumb of a left hand in states that the index finger is horizontal, the thumb is slightly lower than the index finger and the material is vertical to the horizontal plane, holding a double-edge blade by a right hand in states that the blade is horizontal and is above the index finger of the left hand, and pulling the blade from left front direction to right rear direction in parallel, so as to obtain leaf sections containing the main veins. The method has the beneficial effects that the mixing of clamping materials can be avoided; the consumed time is short, the cost is low, the operation is simple, the sections with uniform thickness can be easily obtained, and the cell change of a fresh leaf sample can be conveniently observed; and furthermore, a solvent used in the process is few, and the harm is low.