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Method for identifying rice blast biocontrol bacteria

A technology for auxiliary identification of rice blast fungus, which is applied in the field of identification of rice blast fungus biocontrol, can solve the problems of complex screening method process, long detection and analysis time, low screening utilization rate, etc., and achieve low price, low cost and high control effect small error effect

Inactive Publication Date: 2013-09-04
CHINA AGRI UNIV
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  • Claims
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Problems solved by technology

[0004] The conventional screening method is complex, the detection and analysis time is long, the screening result is quite different from the actual control effect in the field, and the screening utilization rate is low

Method used

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  • Method for identifying rice blast biocontrol bacteria
  • Method for identifying rice blast biocontrol bacteria
  • Method for identifying rice blast biocontrol bacteria

Examples

Experimental program
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Embodiment 1

[0045] Embodiment 1, identification rice blast biocontrol bacteria

[0046] 1. Isolation and activation of test strains

[0047] 1. Isolation of strains to be tested

[0048] Cut the healthy leaves of Rejingyou No. 35 collected in Ciyun Town, Jiangjin District, Chongqing into about 5cm, put it into a 150ml triangular flask filled with 45ml of 75% (volume percentage) alcohol, shake it for 5 minutes; then pour off the alcohol, Add 45ml of sterile water to the triangular flask, soak for 10min, dilute the supernatant according to a certain ratio by dilution separation method, draw 1ml of the diluted solution into a test tube containing 9ml of water, shake and mix well, and obtain 10 -2 diluent, and then prepare 10 respectively according to the above method -3 、10 -4 、10 -5 diluent, respectively take 10 -3 、10 -4 、10 -5 0.2ml of the diluted solution was spread on the NA medium plate, and after drying, it was incubated upside down in a constant temperature incubator at 28°C f...

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Abstract

The invention discloses a method for identifying rice blast biocontrol bacteria. The invention provides a method for identifying or complementarily identifying whether bacteria to be detected are rice blast biocontrol bacteria or not. The method comprises the following steps of: (1) placing a toothpick in a cultivation container paved with wet filtering paper and placing a plant leaf section which is colonized with the bacteria to be detected into the toothpick to be cultivated to obtain a cultivated leaf; (2) puncturing the surface of the cultivated leaf by utilizing the toothpick, wherein a leaf cuticle is not damaged; inoculating a rice blast spore suspension solution to punctured parts to obtain an inoculated leaf; and (3) continually cultivating the inoculated leaf to obtain a leaf to be observed, and observing the leaf to be observed. An experiment of the invention proves that the invention provides the method for identifying or complementarily identifying whether the bacteria to be detected are the rice blast biocontrol bacteria or not; the speed of a testing result of the method is fast; and after the bacteria are inoculated for 48 hours, whether bacillus to be detected resists rice blast bacteria or has the prevention and treatment effects on rice blast or not can be judged.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying rice blast biocontrol bacteria. Background technique [0002] Rice blast is one of the important diseases of rice production in the world. The disease is widely distributed. It occurs in more than 80 countries and regions in the world, and it also occurs in the north and south rice regions of my country. In areas where the disease is prevalent, the yield is generally reduced by 10-20%, and in severe cases it can reach 40-50%. In particularly heavy fields, there is even no harvest. At present, the use of rice blast-resistant varieties and the use of chemical pesticides are still effective measures to control rice blast, but due to the simplification of blast-resistant varieties, the genetic complexity of physiological races of the blast The toxicity of chemical pesticides and the resistance of pathogenic bacteria, rice blast-resistant varieties and the use of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/07C12R1/645
Inventor 王琦沙月霞李燕付学池
Owner CHINA AGRI UNIV
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