Rapid freehand sectioning method for main vein supporting leaves

Abstract

The invention relates to a rapid freehand sectioning method for main vein supporting leaves. The rapid freehand sectioning method comprises the steps of putting collected mature, healthy and complete leaves with no pests and diseases into clear water, washing, soaking leaf stalks into the clean water, taking out leaves, trimming along two sides of main veins until leaves with the widths of 2mm-6mm along two sides of the main veins and the main veins with the lengths of 2cm-6cm are preserved, averagely cutting leaves into two sections, putting two sections of epidermises containing the main veins of the leaves face to face, holding the material by virtue of an index finger and a thumb of a left hand in states that the index finger is horizontal, the thumb is slightly lower than the index finger and the material is vertical to the horizontal plane, holding a double-edge blade by a right hand in states that the blade is horizontal and is above the index finger of the left hand, and pulling the blade from left front direction to right rear direction in parallel, so as to obtain leaf sections containing the main veins. The method has the beneficial effects that the mixing of clamping materials can be avoided; the consumed time is short, the cost is low, the operation is simple, the sections with uniform thickness can be easily obtained, and the cell change of a fresh leaf sample can be conveniently observed; and furthermore, a solvent used in the process is few, and the harm is low.

Description

technical field [0001] The invention relates to the field of botany, in particular to a fast freehand slicing method for main vein supporting leaves. Background technique [0002] Freehand slicing technology is a basic experimental technique commonly used in botany experiments. It can cut fresh materials and observe the natural color and living structure of plant tissues. It is widely used in botany experimental teaching and scientific research. This method is often used to observe the structure of plants and identify the chemical components of plant cell tissues. In recent years, it is also commonly used to detect the expression of reporter genes in gene plants. It is usually required that the material has a suitable hardness and size. For materials that are too hard or too soft, it is relatively difficult to slice by hand. Among them, the blade is relatively difficult to slice by hand, because the blade is relatively soft, and most of them cannot be directly sliced ​​by ha...

AI Technical Summary

Problems solved by technology

The advantage is that it is very universal, and the disadvantage is that it is easy to mix the materials of the clamping objects; the second is to use three blades to bundle, and the middle blade is indented by a few millimeters, and the blade is cut into thin slices by using the indented gap. The advantage is to avoid The disadvantage of mixing clamp materials is that three blades are required, which is expensive, and multiple blades are bundled, which also increases safety risks. The thickness is rated as the thickness of the blade, and the thickness cannot be adjusted according to needs, so there are certain requirements for the material

The third is to use tweezers to hold the material for freehand slicing. This method is not very operable and it is not easy to cut thinly; the fourth is to cut in parallel with the index finger, which is similar to the usual method of cutting vegetables. By cutting back and forth , the small retraction of the index finger to obtain the thin slice of the specimen, the advantage is that no clamp is required; the disadvantage is that the safety risk is high, it is not easy to cut thin, and there are also requirements for materials

If it is a leaf, you can roll it tightly. The cut material should not be too long, about 2-2.5cm. At this time, use the index finger, middle finger and thumb to pinch the material together. Do not use too much force when pinching the young material. In order not to damage the tissue cells, hold one side of the double-sided blade with the thumb and index finger of the right hand, keep the wrist flat, and keep the front end of the blade vertically connected to the material to be cut, first cut the material flat, and then slice continuously inward with the wrist , as long as the blade catches the material, it can slice vertically. The advantage is that no clamp is needed; the disadvantage is that the cost is high, and the operation is difficult, it is not easy to master, and there are requirements for the material

The sixth type is for leathery leaves, you can stack multiple leaves of the same size together, pinch them, and cut them together. The disadvantage is that they are easy to loosen and not easy to cut thinly; In a cylindrical shape, pinch and cut together, the disadvantage is that it is not easy to cut thin and even cut

The eighth method is the paraffin section method. Although the obtained specimens can be stored permanently, the production process is long, time-consuming, costly, low in acquisition rate, and uses a large amount of volatile organic reagents, which is harmful to the human body and the environment. In addition, highly toxic and strong solvents are used in the process of making tablets, and many small structures or components are dissolved, which are easily lost in the process of dehydration and transparency

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