The invention relates to a preparation method of paraffin slices of eggplant root stalk part tissues, and belongs to the technical field of biological histology. The method comprises the eleven steps of fixing, softening, dewatering, transparency treatment, wax soaking, coating and burying, wax trimming and slicing, slice unfolding and slice baking, wax removal, rehydration, dyeing, slice sealing and observation. The method belongs to a fast, convenient, safe and practical eggplant root stalk part tissue paraffin slice method. The result obtained by the method realizes high operability, high repeatability, high stability and high reliability. Through the microscopic observation, the slice quality is high; the effect is good; the tissue structure is clear. The method can be used for studying, observing and judging the form structure change of the eggplant root stalk part tissues, so that the physiological, pathological and morphological study can be performed on the eggplants. The method can also be widely applied to tissue slice experiments of eggplant fruits or even other plants; good scientific research application prospects are realized.