Preparation method of paraffin slices of eggplant root stalk part tissues

A paraffin section and tissue technology, applied in the field of biological histology, can solve the problems of less research on paraffin section technology, incomplete section fragmentation, unsatisfactory effect, etc., and achieves shortened fast green staining time, clear tissue structure, and uniform wax soaking effect. Effect

Inactive Publication Date: 2017-09-15
云南省农业科学院园艺作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are few researches on paraffin section technology on eggplant, and the effect is not ideal (the section is broken and the clarity is poor)

Method used

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  • Preparation method of paraffin slices of eggplant root stalk part tissues
  • Preparation method of paraffin slices of eggplant root stalk part tissues
  • Preparation method of paraffin slices of eggplant root stalk part tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A kind of preparation method of the paraffin section of eggplant rhizome tissue, comprises the steps:

[0051] Step (1) fixation and softening:

[0052] Select fresh eggplant plants, intercept the stem tissue at 1-3 cm above the ground and the main root tissue at 1-3 cm below the ground as test materials for paraffin sections;

[0053] Rinse the selected test materials with running water to remove impurities, cut them into small pieces of 0.48-0.52 cm in length, put them into FAA fixative solution, and fix them at 3°C ​​for 3 days; each 100mL of FAA fixative solution contains 5mL of formalin, ice 5mL of acetic acid, 90mL of ethanol with a volume concentration of 70%; the volume of FAA fixative used for fixation is more than 20 times the volume of the test material.

[0054] Afterwards, add glycerin with a volume equal to 5% of the volume of the fixative solution to the fixative solution to soften the test material for the first time. After 5 days, take the test materia...

Embodiment 2

[0069] A kind of preparation method of the paraffin section of eggplant rhizome tissue, comprises the steps:

[0070] Step (1) fixation and softening:

[0071] Select fresh eggplant plants, intercept the stem tissue at 1-3 cm above the ground and the main root tissue at 1-3 cm below the ground as test materials for paraffin sections;

[0072] Rinse the selected test materials with running water to remove impurities, cut them into small pieces of 0.48-0.52 cm in length, put them into FAA fixative solution, and fix them at 5°C for 5 days; each 100mL of FAA fixative solution contains 5mL of formalin, ice 5mL of acetic acid, 90mL of ethanol with a volume concentration of 70%; the volume of FAA fixative used for fixation is more than 20 times the volume of the test material.

[0073] After that, add glycerol with a volume of 5% of the volume of the fixative solution to the fixative solution to soften the test material for the first time. After 7 days, take the test material out an...

Embodiment 3

[0090] A kind of preparation method of the paraffin section of eggplant rhizome tissue, comprises the steps:

[0091] Step (1) fixation and softening:

[0092] Select fresh eggplant plants, intercept the stem tissue at 1-3 cm above the ground and the main root tissue at 1-3 cm below the ground as test materials for paraffin sections;

[0093] Rinse the selected test materials with running water to remove impurities, cut them into small pieces of 0.48-0.52 cm in length, put them into FAA fixative solution, and fix them at 4°C for 4 days; each 100mL of FAA fixative solution contains 5mL of formalin, ice 5mL of acetic acid, 90mL of ethanol with a volume concentration of 70%; the volume of FAA fixative used for fixation is more than 20 times the volume of the test material.

[0094] Afterwards, add glycerol with a volume equal to 5% of the volume of the fixative solution to the fixative solution to soften the test material for the first time. After 6 days, take the test material ...

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Abstract

The invention relates to a preparation method of paraffin slices of eggplant root stalk part tissues, and belongs to the technical field of biological histology. The method comprises the eleven steps of fixing, softening, dewatering, transparency treatment, wax soaking, coating and burying, wax trimming and slicing, slice unfolding and slice baking, wax removal, rehydration, dyeing, slice sealing and observation. The method belongs to a fast, convenient, safe and practical eggplant root stalk part tissue paraffin slice method. The result obtained by the method realizes high operability, high repeatability, high stability and high reliability. Through the microscopic observation, the slice quality is high; the effect is good; the tissue structure is clear. The method can be used for studying, observing and judging the form structure change of the eggplant root stalk part tissues, so that the physiological, pathological and morphological study can be performed on the eggplants. The method can also be widely applied to tissue slice experiments of eggplant fruits or even other plants; good scientific research application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of biological histology, and in particular relates to a method for preparing paraffin slices of eggplant rhizome tissue. Background technique [0002] Paraffin tissue section technology is the most widely used method in routine histological preparation techniques. It is not only used to observe the morphological structure of normal cell tissue, but also the main method used in pathology and forensic science to study, observe and judge the morphological changes of cell tissue, and has been widely used in research in many disciplines. However, due to the specific tissue structure of plants, the specific operation methods of different materials are also different. [0003] At present, there are few researches on the paraffin section technology on eggplant, and the effect is not ideal (the section is broken and the clarity is poor). Therefore, how to overcome the deficiencies of the prior art is an urgent prob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/30G01N1/36
CPCG01N1/28G01N1/2813G01N1/30G01N1/36
Inventor 吴丽艳龚亚菊鲍锐黎志彬
Owner 云南省农业科学院园艺作物研究所
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