The invention provides a virtual microscope based on visual perception and application thereof, and belongs to the field of experimental equipment. The virtual microscope based on the visual perception comprises: a microscope body model; a rotation sensor, a remote communication module, a display, a camera, and an electronic chip disposed on the microscope body model; and a computing and display device. The rotation sensor, the remote communication module, the display, and the camera are respectively connected to the electronic chip. The remote communication module is capable of communicatingwith the computing and display device. On the one hand, according to the virtual microscope based on the visual perception and the application thereof, a virtual fusion technology is utilized to perform information enhancement on a user observation result, which is beneficial for the user to randomly explore the process, the mechanism, and the principle of the experimental phenomena; on the otherhand, according to the virtual microscope based on the visual perception and the application thereof, the operating experience under a real microscope condition is obtained through the physical operation, which helps an experimenter master relevant experimental skills.