Horned holly fungal endophyte-harzianum L

A technology of bacteria strains and microorganisms, applied in the field of microorganisms, can solve problems such as the types of unsafe microbial source pesticides, and achieve excellent results

Inactive Publication Date: 2007-09-19
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of endophytic fungus-Trichoderma harzianum strain L that isolates and screens out from the Chinese wolfberry tissue for the deficiency that chemical pesticides are unsafe and the types of microbial source pesticides are few; Another purpose of the present invention is to provide Using the strain L to ferment and extract its metabolites to prepare microbial pesticides for preventing and treating some plant fungal diseases and its preparation method

Method used

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  • Horned holly fungal endophyte-harzianum L
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Effect test

Embodiment 1

[0025] Embodiment 1: (isolation and screening of endophytic fungus Trichoderma harzianum strain L)

[0026] The present invention is obtained from Chinese wolfberry stems collected in Tianmu Mountain, Lin'an, Zhejiang Province in May 2006, through the steps of surface disinfection, separation, cultivation and fermentation of endophytic fungi, activity determination, and screening of effective bacterial strains for inhibiting plant fungal disease pathogens. Endophyte Trichoderma harzianum Rifai strain L, preserved.

[0027]Determination of the antibacterial activity of Trichoderma harzianum Rifai strain L: Take 1 mL of its fermentation supernatant in a sterile petri dish, mix it with 9 mL of PDA medium cooled to 50 ° C, and put it in each medium after cooling Place a 4mm-diameter bacteria cake for test bacteria such as Cucumber wilt, Bean anthracnose, Watermelon Fusarium, Cucumber blight and Tomato blight respectively, and the cake is connected to the center of the petri dish (...

Embodiment 2

[0030] Embodiment 2: (the preparation method of Trichoderma harzianum strain L fermentation metabolite)

[0031] Follow these steps:

[0032] (1) Activation culture on the slant of the strain test tube: the slant medium is potato dextrose medium (PDA), and its components and contents are: 200g of potatoes, 20g of glucose, and 20g of agar; first peel the potatoes, cut them into pieces and boil for half an hour , then filter with gauze, add glucose and agar, and make up water to 1L after dissolving; under sterile conditions, pick up a little bacteria of Trichoderma harzianum Rifai strain L with an inoculation needle, to a sterilized solid Put in a PDA medium test tube (15×150mm), put it in an incubator at 28°C±1°C, activate and cultivate for 96 hours, until the inclined surface of the test tube is covered with green spores;

[0033] (2) Seed liquid culture: seed medium is potato glucose liquid medium, and its component and content are: potato 200g, glucose 20g; After dissolvin...

Embodiment 3

[0037] Embodiment 3: (Utilize Trichoderma harzianum strain L fermentation metabolite to prepare into wettable powder)

[0038] in:

[0039] White carbon black: (produced by Shanghai Fuqi Chemical Co., Ltd.)

[0040] Nongdai 0203-B: (produced by Xingtai Lanxing Auxiliary Factory)

[0041] Sodium lignosulfonate: (produced by Shanghai Weicheng Chemical Co., Ltd.)

[0042] Light calcium carbonate: (produced by Shanghai Daewoo Biochemical Co., Ltd.)

[0043] The product contains 1.5% sesquiterpenoids Trichothec-9-en-4-o1, 12,13-epoxy-, acetate, (4β)-(8CI, 9CI), adding 2% white carbon black as an adsorbent, and Add 1% agricultural milk 0203-B and 3% sodium lignosulfonate auxiliary materials, and finally make up to 100% with light calcium carbonate. A").

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Abstract

The invention relates to microbe technique field, endophytic fungus of Chinese Holly of the invention collected from Chinese Holly living plant of Tianmu Mountain of Linan City is obtained by endophytic fungus microbe separation technology, named as Trichoderma harzianum Rifai stem L identified by microbial taxonomy stored by general microbilogy centre of China Forestry Culture Collection Center on date of august 14th 2006, registration number is CGMCC NO.1780. The most prominent feature is the stem can generate activated component seaquiterpenoids Trichothec-9-en-4-ol,12,13-epoxy-,acetate,(4beta)-(8CI,9CI) by fermentation for inhibiting phytopathogenic fungi bacteria, it is an important microbe resource in agriculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the isolation of effective strains of plant endophytic fungi for preventing and controlling some plant fungal diseases, and the preparation and application methods of antibacterial metabolites thereof. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological control, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04
Inventor 俞晓平申屠旭萍
Owner CHINA JILIANG UNIV
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