Dendrobium officinale Kimura et Migo endophytic fungus and applications thereof

Abstract

The invention discloses dendrobium officinale Kimura et Migo endophytic fungus. The dendrobium officinale Kimura et Migo endophytic fungus is obtained by separating from the living body of dendrobium officinale Kimura et Migo belonging to dendrobium species, orchidaceae by adopting an endophytic fungus separation and purification technology; and identified by the microbial taxonomy, the dendrobium officinale Kimura et Migo endophytic fungus is Pestalotiopsis sp.DO14; the strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M 2015180. Through co-culture with dendrobium officinale Kimura et Migo tissue culture seedlings, the steam thickening and lengthening, and the stem and leaf color deepening of the dendrobium officinale Kimura et Migo tissue culture seedlings can be promoted, the improvement of the polysaccharide content in the stem can be promoted, the change of alcohol soluble ingredients in the stem and the flavonoid ingredients in the leaves can be promoted; the dendrobium officinale Kimura et Migo endophytic fungus is an important microbe closely related to the growth and metabolites of dendrobium officinale Kimura et Migo, and has the application potential of promoting the growth of dendrobium officinale Kimura et Migo and the change of the metabolic components.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to endophytic fungi of Dendrobium officinale and applications thereof. Background technique [0002] Dendrobium officinale Kimura et Migo (Dendrobium officinale Kimura et Migo) is a precious Chinese medicinal material in my country. It has the functions of nourishing yin and clearing heat, nourishing stomach and promoting body fluid, moistening lung and improving eyesight, anti-cancer and anti-aging, etc. head. The 2010 edition of "The Pharmacopoeia of the People's Republic of China" delineated Dendrobium officinale from the Dendrobium medicinal materials and included it separately. With the breakthrough of Dendrobium candidum cultivation technology, the industry has developed rapidly. The current planting area of ​​Dendrobium candidum in China has exceeded 40,000 mu, and the output value has exceeded 5 billion yuan. It has become one of the largest and fastest-growing Chines...

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Problems solved by technology

However, there is a lack of research on medicinal orchids, especially the endophytic fungi of Dendrobium. Endophytic fungi have b

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