Eucommia ulmoides endogenous bacillus pumilus and application thereof

A technology of Bacillus pumilus and bacteria, which is applied to Bacillus pumilus and its application field in preventing and controlling plant diseases, can solve the problems of irrational use of chemical pesticides, environmental pollution of chemical pesticides, poisoning of humans and animals, etc., and achieves good development and application prospects. Simple culture conditions and growth-inhibiting effect

Active Publication Date: 2015-07-22
ANHUI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the long-term over-reliance on chemical pesticides, especially the use of some pesticides with long residual effect and high toxicity, and the unreasonable use ...

Method used

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  • Eucommia ulmoides endogenous bacillus pumilus and application thereof
  • Eucommia ulmoides endogenous bacillus pumilus and application thereof
  • Eucommia ulmoides endogenous bacillus pumilus and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: the acquisition of Bacillus pumilus DZBT01 (Bacillus pumilus DZBT01)

[0030] The present invention collected the root tissue of the eucommia plant in the campus of Anhui Agricultural University, Hefei City, Anhui Province in October 2013. The specific separation and purification steps are as follows:

[0031] (1) collecting the root tissue of a healthy Eucommia plant;

[0032] (2) Surface disinfection and sterility detection of the root tissue of the Eucommia kambifolia plant: soak the roots collected in step (1) for 1 min in alcohol with a concentration of 75%, and soak for 1-2 min in a sodium hypochlorite solution with a concentration of 1%; Rinse the soaked roots three times with sterile water, and dry them in the shade to obtain sterilized roots. Using the gradient dilution method, quantitatively weigh 1 g of the sterilized roots, add 0.01mol / L PBS buffer solution to constant volume to 10 mL, and grind to slurry to obtain 10 -1 Dilute the sample sus...

Embodiment 2

[0038] Embodiment 2: Bacillus pumilus DZBT01 (Bacillus pumilus DZBT01) to the bacteriostasis of five kinds of plant pathogenic fungi

[0039] (1) Cultivation of Bacillus pumilus DZBT01

[0040] Bacillus pumilus DZBT01 (Bacillus pumilus DZBT01), an endophytic bacterium of Eucommia pumilus, was activated by inoculating it with beef extract peptone agar solid medium, and placed in a constant temperature incubator at 37°C for constant temperature cultivation for 24 hours.

[0041] The formula of the beef extract-peptone solid medium is: 10g of peptone, 3g of beef extract, 5g of sodium chloride, 15g of agar, and 1L of distilled water. Before use, the beef extract peptone solid medium was sterilized at a temperature of 121°C and a pressure of 0.1MPa for 20 minutes, and then left to cool for later use.

[0042] (2) Cultivation of plant pathogenic fungi

[0043] The slant culture of four plant pathogenic fungi: Rhizoctonia cerealis, Gaeumannomyces gramini, Fusarium moniliforme and F...

Embodiment 3

[0053] Embodiment 3: the control situation of bacillus pumilus DZBT01 (Bacillus pumilus DZBT01) to greenhouse potted wheat sheath blight

[0054] Sterilize the surface of wheat seeds (Yangmai 158) with 1% sodium hypochlorite for 10 minutes, rinse with sterile water 3-5 times, then place them in a petri dish covered with sterile wet filter paper, and place them in a constant temperature incubator at 25°C. Accelerate the germination and wait for the seeds to germinate for later use. The wheat field soil is mixed with an appropriate amount of fine sand, and dry heat sterilized to obtain sterilized wheat field soil, which is ready for use. Mix the wheat sheath blight bacteria cultivated on the barley sand medium with the sterilized wheat field soil in a ratio of 1:5 (W / V), and put it into a pot (16 cm high, 13 cm in upper diameter, 6 cm in lower diameter). )middle. Soak the surface-sterilized and germinated wheat grains in the culture solution of Bacillus pumilus DZBT01 (the bac...

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Abstract

The invention relates to the technical field of microbes. The eucommia ulmoides endogenous bacteria are separated from eucommia ulmoides living plants by adopting an endogenous bacteria microbe separation technology. Through identification by microbial taxonomy, strain is named Bacillus pumilus DZBT01 and is collected in China General Microbiological Culture Collection Center, the collection number is January 30, 2015, and the collection register number is CGMCC NO.10470. The strain disclosed by the invention has the characteristics that the strain can achieve an obvious effect of inhibiting plant pathogenic fungi. The invention discloses an application of the Bacillus pumilus DZBT01 in control of wheat sharp eyespot and an application of Bacillus pumilus in promotion of wheat growth. The Bacillus pumilus DZBT01 disclosed by the invention refers to eucommia ulmoides plant endogenous bacteria, is simple in culture conditions and easy to preserve and refers to an agriculturally important microbial resource.

Description

technical field [0001] The invention belongs to the field of plant protection, in particular to a bacillus pumilus and its application in preventing and treating plant diseases. Background technique [0002] As an effective means of controlling harmful organisms, chemical pesticides have played an important role in integrated pest control and ensuring stable and high yields of crops such as agriculture and forestry. However, the long-term over-reliance on chemical pesticides, especially the use of some pesticides with long residual effect and high toxicity, and the unreasonable use of chemical pesticides in some areas have led to problems such as environmental pollution, human and animal poisoning, and pesticide residues in the use of chemical pesticides. At present, the hazards of chemical control are gradually being recognized by people, and the development of new biopesticides that are safe, efficient, low-toxic, and low-residue has become a new hot spot in the field of p...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P21/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 丁婷韩亚惠陈方新苏博龙建
Owner ANHUI AGRICULTURAL UNIVERSITY
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