Microbial fertilizer as well as preparation method and application thereof
A technology of microbial fertilizer and microbial inoculum, which is applied in the field of microbial fertilizer and its preparation, can solve the problems of not killing pathogenic bacteria from the root, affecting the growth of crops, treating the symptoms but not the root cause, etc., so as to prevent and cure tomato canker and reduce planting Cost, effect Long-term effective effect
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Embodiment 1
[0019] Screening of functional microorganisms
[0020] In the area where tomato canker disease occurs, select the areas with less disease to collect soil samples, carry out the separation, culture and preservation of soil microorganisms, and initially screened and isolated 7 strains, numbered A01-A07. At the same time from the laboratory strain library 9 strains of Bacillus subtilis, Bacillus Laterosporus, Bacillus jelly-like, Trichoderma harzianum, Verticillium, Streptomyces jingyang, Paecilomyces lilacinus, Trichoderma yellow, and powdery parasitic bacteria were selected for the prevention and control of plant diseases Strains. 7 strains of preliminary screening strains and 9 strains of laboratory strains were tested for bacteriostasis to observe the inhibitory effect of each strain on the pathogenic bacteria of tomato canker. The specific results are as follows:
[0021] Screening strains
Laboratory bacteria
A01
-
Bacillus subti...
Embodiment 2
[0025] Verify the effect of microbial agents in killing pathogenic bacteria through antibacterial experiments
[0026] A: Preparation of microbial agents. Select Bacillus Laterosporus, Streptomyces jingyang, Streptomyces lilac Grey, Streptomyces aureus, Bacillus jelly-like and Trichoderma viride in a ratio of 1:1:1:1:1:1, and dilute with sterile distilled water The amount of viable bacteria is about 2000 / mL of the bacterial liquid, and then the bacterial liquid is diluted 100 times, 40 times, 20 times, 10 times, 5 times, and 2 times for use.
[0027] B: Preparation of pathogenic bacteria plates. Use sterile distilled water to gradually dilute the pathogenic bacteria into a pathogenic bacteria liquid with a viable amount of about 2000 bacteria / mL, and then pipette 0.1 mL of the pathogenic bacteria liquid into each culture plate, and spread evenly until there is no obvious liquid on the surface.
[0028] C: Separately draw 0.1 mL of different concentrations of microbial inoculants in...
Embodiment 3
[0034] Preparation of microbial fertilizer
[0035] A: Mix Bacillus Laterosporus, Streptomyces Jingyang, Streptomyces lilac Grey, Streptomyces aureus, Bacillus jelly-like and Trichoderma viride in a ratio of 1:2:2:2:1:2 to produce microbial bacteria Agent.
[0036] B: Mix molasses, hydrolyzed feather meal, and calcium chloride in a ratio of 60:35:5 to prepare a nutrient.
[0037] C: Crush the straw, rice husk, and wood chips separately, mix them evenly according to the ratio of 50:30:20, and then decompose through fermentation, then dry at low temperature to prepare the carrier.
[0038] D: Mix the microbial inoculum, nutrient and carrier uniformly in the ratio of 5:25:60, measure the moisture content, and then add an appropriate amount of water to adjust the moisture content to about 15%, which is the prepared microbial fertilizer. The amount of viable bacteria in gram of microbial fertilizer is 2.23×10 8 One.
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