Microbial fertilizer as well as preparation method and application thereof

A technology of microbial fertilizer and microbial inoculum, which is applied in the field of microbial fertilizer and its preparation, can solve the problems of not killing pathogenic bacteria from the root, affecting the growth of crops, treating the symptoms but not the root cause, etc., so as to prevent and cure tomato canker and reduce planting Cost, effect Long-term effective effect

Inactive Publication Date: 2018-05-29
DALIAN UNIV OF TECH QINGDAO NEW ENERGY MATERIALS TECH RES INST CO LTD
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention of tomato canker is mainly based on prevention. Doing a good job of prevention in the early stage of the disease will have a better effect on the control of the disease. The methods adopted mainly include measures such as strengthening quarantine, seed treatment, soil treatme

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Screening of functional microorganisms

[0020] In the area where tomato canker disease occurs, select the areas with less disease to collect soil samples, carry out the separation, culture and preservation of soil microorganisms, and initially screened and isolated 7 strains, numbered A01-A07. At the same time from the laboratory strain library 9 strains of Bacillus subtilis, Bacillus Laterosporus, Bacillus jelly-like, Trichoderma harzianum, Verticillium, Streptomyces jingyang, Paecilomyces lilacinus, Trichoderma yellow, and powdery parasitic bacteria were selected for the prevention and control of plant diseases Strains. 7 strains of preliminary screening strains and 9 strains of laboratory strains were tested for bacteriostasis to observe the inhibitory effect of each strain on the pathogenic bacteria of tomato canker. The specific results are as follows:

[0021] Screening strains

Antibacterial effect

Laboratory bacteria

Antibacterial effect

A01

-

Bacillus subti...

Embodiment 2

[0025] Verify the effect of microbial agents in killing pathogenic bacteria through antibacterial experiments

[0026] A: Preparation of microbial agents. Select Bacillus Laterosporus, Streptomyces jingyang, Streptomyces lilac Grey, Streptomyces aureus, Bacillus jelly-like and Trichoderma viride in a ratio of 1:1:1:1:1:1, and dilute with sterile distilled water The amount of viable bacteria is about 2000 / mL of the bacterial liquid, and then the bacterial liquid is diluted 100 times, 40 times, 20 times, 10 times, 5 times, and 2 times for use.

[0027] B: Preparation of pathogenic bacteria plates. Use sterile distilled water to gradually dilute the pathogenic bacteria into a pathogenic bacteria liquid with a viable amount of about 2000 bacteria / mL, and then pipette 0.1 mL of the pathogenic bacteria liquid into each culture plate, and spread evenly until there is no obvious liquid on the surface.

[0028] C: Separately draw 0.1 mL of different concentrations of microbial inoculants in...

Embodiment 3

[0034] Preparation of microbial fertilizer

[0035] A: Mix Bacillus Laterosporus, Streptomyces Jingyang, Streptomyces lilac Grey, Streptomyces aureus, Bacillus jelly-like and Trichoderma viride in a ratio of 1:2:2:2:1:2 to produce microbial bacteria Agent.

[0036] B: Mix molasses, hydrolyzed feather meal, and calcium chloride in a ratio of 60:35:5 to prepare a nutrient.

[0037] C: Crush the straw, rice husk, and wood chips separately, mix them evenly according to the ratio of 50:30:20, and then decompose through fermentation, then dry at low temperature to prepare the carrier.

[0038] D: Mix the microbial inoculum, nutrient and carrier uniformly in the ratio of 5:25:60, measure the moisture content, and then add an appropriate amount of water to adjust the moisture content to about 15%, which is the prepared microbial fertilizer. The amount of viable bacteria in gram of microbial fertilizer is 2.23×10 8 One.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to microbial fertilizer as well as a preparation method and application thereof, and belongs to the field of comprehensive control of plant soil-borne diseases. The microbial fertilizer comprises a microbial agent, a nutritional agent, a carrier and water; the microbial agent comprises bacillus laterosporus, streptomyces jingyangensis, streptomyces lavendulae, streptomyces aureofaciens, bacillus mucilaginosus and trichoderma viride. The microbial fertilizer provided by the invention contains functional microorganisms capable of inhibiting and killing pathogenic bacteria in soil, can effectively prevent and treat tomato canker by a method for inhibiting bacteria by bacteria and can improve the soil healthy environment; germs can be killed from the soil root, various ways of seeds, seedlings, irrigating water, farm tools and the like can be blocked, and pathogen transmission can be avoided; the microbial fertilizer adopts natural microorganisms in the natural world,is green and free of pollution and has long-term effect; the use of pesticide is reduced, the planting cost is reduced and environmental pollution is reduced.

Description

Technical field [0001] The invention relates to a microbial fertilizer and a preparation method and application thereof, belonging to the field of comprehensive prevention and control of plant soil-borne diseases. Background technique [0002] Tomato canker is one of the most serious diseases in tomato production. Since it was first reported in the United States, it has rapidly spread and spread to many major tomato producing areas in the world, and has gradually become a worldwide disease. At present, the disease is widespread in North America, Europe, Asia, Africa, Oceania, and covers more than 60 countries including the United States, Britain, Canada, Germany, and Japan. my country first discovered tomato canker in Pinggu, Beijing in 1985, and it has now spread to many tomato producing areas. The occurrence of tomato canker in Northeast my country was confirmed in 1993 for the first time. In 2002, the incidence of tomato in Fangtang Township, Guoning City, Anhui Province exce...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05C11/00C05F5/002C05G3/00C05G3/80C05F11/00C05F17/00C05D3/00C05F9/04
Inventor 祝玉洪陈兰池明张润德石佳波姜大川
Owner DALIAN UNIV OF TECH QINGDAO NEW ENERGY MATERIALS TECH RES INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap