The invention relates to the technical field of gynecological cleaning solutions, in particular to a gynecological lotion and a preparation method thereof. The gynecological lotion at least comprisesthe following components in parts by weight: 3-10 parts of a humectant, 0.1-3 parts of a skin conditioner, 1-5 parts of a traditional Chinese medicine extract, 0.1-0.5 part of an ecological nutrient solution, 0.1-0.5 part of compound essential oil, 0.1-3 parts of a solubilizer, 0.01-0.3 part of a coloring agent and the balance of a solvent, totaling 100 parts. The raw materials are rich in medicine sources, reasonable in component compatibility, synergistic, free of any side effect and irritation and capable of forming a layer of thin mucous film on the surface of the skin after making contactwith the skin, various medicines are wrapped in the mucous film, the medicines can make full contact with the skin, and the medicine effect is improved; the gynecological lotion has the effects of clearing away heat and toxic materials, killing viruses, relieving itching, resisting bacteria, diminishing inflammation, preventing mildew, deodorizing, promoting the secretion of saliva or body fluid,nourishing yin and stopping leucorrhea, can stimulate regeneration of tissue cells or vagina damaged mucosa, improves self-repairing capacity and resistance, and effectively enhances the immunity oforganisms.