31 results about "Streptomyces lavendulae" patented technology

The invention discloses a streptomyces lavendulae DTJ-24 of which the collection number is CGMCC No.9972, an antibiological inoculants containing the streptomyces lavendulae, and application of the antibiological inoculant to control of a strawberry anthracnose. After being activated, the streptomyces lavendulae DTJ-24 is inoculated in a seed medium at 20% of inoculation quantity, and is cultivated for 3 days at the speed of 120 r / min at 25 DEG C to obtain a seed solution; the seed solution is inoculated in a fermentation medium at 4% of inoculation quantity, the quantity of the mediums in a fermentation tank is 70%, the fermentation condition is that the temperature is 25 DEG C, the throughput is 18%, the stirring speed is 120 r / min, the fermentation time is 3 days, and the fermentation broth and a base material are equivalently and uniformly mixed and dried to prepare the antibiological inoculant. The foliage spray or transplantation is carried out on the antibiological inoculant, through the root-irrigation treatment, the strawberry anthracnose can be effectively controlled, and the control effect reaches 78.3%. The antibiological inoculant is simple in preparation process, low in cost, and convenient to use, can reduce the use of chemical pesticides and pesticide residues on fruit during the strawberry cultivation process, and has the advantages of safety and environmental protection.

The invention relates to the field of organic fertilizer, in particular to special bio-organic fertilizer for pepper and a preparation method thereof. The special bio-organic fertilizer is prepared from 15-30 parts of complex microbial inoculants and 70-85 parts of a carrier, wherein the complex microbial inoculants contain paenibacillus polymyxa 2.5-15 hundred million cfu / g, trichoderma viride 1-5.0 hundred million cfu / g and streptomyces lavendulae 0.5-4 hundred million cfu / g. The carrier is formed by microorganism composting and fermenting of mushroom residues, rapeseed meal, peanut meal, castor bean leaves and peach leaves. The castor bean leaves and peach leaves contained in the bio-organic fertilizer prevent soil-borne disease functional strains and promote crop growth strains, and a substrate for usage is fermented mixed organic fertilizer. The special bio-organic fertilizer for the pepper is in a powder shape, is used as base fertilizer, provides nutrients needed for growth of the pepper, can effectively improve soil, prevent crop soil-borne disease infection and prevent and treat soil insect mole crickets, grubs, cutworms and others of the pepper and is capable of obviously improving the quality of the pepper and increasing the yield of the pepper.

The invention relates to a liquid state composite biological multivariable control paddy herbicide. The herbicide is characterized by being prepared from medicinal plants and multiple species inoculant. The medicinal plants comprise, by weight, 5-25 parts of radix euphorbiae lantu, 5-25 parts of cumin, 5-25 parts of garlic, 5-25 parts of radix angelicae pubescentis and 5-25 parts of common cnidium fruits. The multiple species inoculant comprises, by weight, 5-20 parts of bacterial liquid of streptomyces lavendulae, 5-20 parts of bacterial liquid of streptomyces fradiae, 5-20 parts of bacterial liquid of streptomyces viridocbromogenes, 5-20 parts of bacterial liquid of streptomyces griseolus, 5-20 parts of bacterial liquid of alternaria, 5-20 parts of bacterial liquid of botryis cinerea and 5-20 parts of bacterial liquid of curvuloria lunata. The biological multivariable control paddy herbicide is efficient, low in toxicity, environmentally friendly and not likely to produce resistance and particularly has the remarkable restraining effect on barnyard grass, nutgrass flatsedge, semen euphorbiae and duckweed.

The invention relates to microbial fertilizer as well as a preparation method and application thereof, and belongs to the field of comprehensive control of plant soil-borne diseases. The microbial fertilizer comprises a microbial agent, a nutritional agent, a carrier and water; the microbial agent comprises bacillus laterosporus, streptomyces jingyangensis, streptomyces lavendulae, streptomyces aureofaciens, bacillus mucilaginosus and trichoderma viride. The microbial fertilizer provided by the invention contains functional microorganisms capable of inhibiting and killing pathogenic bacteria in soil, can effectively prevent and treat tomato canker by a method for inhibiting bacteria by bacteria and can improve the soil healthy environment; germs can be killed from the soil root, various ways of seeds, seedlings, irrigating water, farm tools and the like can be blocked, and pathogen transmission can be avoided; the microbial fertilizer adopts natural microorganisms in the natural world,is green and free of pollution and has long-term effect; the use of pesticide is reduced, the planting cost is reduced and environmental pollution is reduced.

The invention discloses actinomycetes for preventing and treating peanut diseases and application of actinomycetes .The name of strain LX14 is Streptomyces lavendulae, the preservation unit is Center of general microbiology of China Committee for Culture Collection of Microorganisms, the preservation time is January 22, 2014, and the preservation number is CGMCC No.8806 .Metabolin Streptomyces lavendulae is screened out from soil in a separation mode, is high in restraining effect on peanut fungi, is applied to preventing and treating peanut crown rot pathogen, a peanut scab disease, peanut root rot and peanut southern blight, has important significance in peanut disease preventing and treating and is very important in plant disease biological preventing and treating; a new approach is added for development of agricultural fungicide, and the Streptomyces lavendulae has good development and application prospects.

The invention relates to a new kind of herbicide with a effective ingredient which is primarily extracted from the metabolic of Streptomyces Lavendulae. Firstly the active herbicide ingredient is separated from the cultivated microorganism of the (Streptomyces Lavendulae) CGMCC No.1368. Then the final herbicide is formed with the selected carrier and auxiliary agent. Such herbicide is advanced in the low dosage, broad-spectrum effect and safety to the environment. It could be a high efficient herbicide both in the paddy field and glebe.

The invention relates to a Streptomyces lavendulae strain, which is named as Streptomyces lavendulae in classification. The Streptomyces lavendulae strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.7548. The invention also relates to application of the Streptomyces lavendulae to preparation of algae microbial fertilizer. The Streptomyces lavendulae strain provided by the invention has a prominent degrading effect on algae residues; waste residues generated after seaweed gel is extracted by the algae processing industry can be recycled, and antibiotic metabolic active substances generated in the fermenting process of the Streptomyces lavendulae can effectively inhibit growth of various pathogenic microorganisms; the Streptomyces lavendulae has excellent effects of promoting growth and increasing yield and has an obvious effect in agricultural production.

The invention discloses preparation of fibrin magnetic micrometer material for separating mRNA and application thereof. The invention adopts a gene engineering technology to directionally clone the streptavidin and the gene of streptomyces lavendulae to pET series carrier which contains fibrin binding domain label, builds difunctional fusion protein CBD-SA carrier with biotin combination activityand fibrin combination activity, transfers E.coli BL21 competent bacterial strain, is expressed by means of IPTG low-temperature induction, and obtains high-content CBD-SA fused expression protein. CBD-SA is taken as biological bridging agent, the traditional physical adsorption method and a chemical bonding method are replaced by a biological specificity coupling method, so that the fibrin magnetic micrometer material is actived, agent with high-effeceicy combination biotinylation can be obtained after the activation, and magnetic micrometer material for separating protein or nucleic acid canbe obtained. The fibrin magnetic micrometer material can be used for separating and distilling the mRNA in biological samples, etc.

The invention discloses an organic compound-inoculant insecticide-fertilizer for preventing and treating lepidoptera pests on paddy rice. The organic compound-inoculant insecticide-fertilizer comprises organic fertilizer and six biological compound inoculants which are composed of Trichoderma viride, Bacillus licheniformis, Streptomyces spirolavendulae, Bacillus subtilis, Bacillus thuringiensis and Candida. The organic compound-inoculant insecticide-fertilizer provided by the invention can improve the disease resistance and stress resistance of rice plants, prevent and treat lepidoptera pests, and prevent and control rice blast and sheath blight of paddy rice, and has an action period of about 40 days.

The invention relates to a method for screening a strain with high yield of Zhongshengmycin and aims at solving the technical problem of relatively low content of Zhongshengmycin in an existing strains. The starting strain is Streptomyces lavendulae var.hainanensis new var. The method comprises the following steps: germinating mycelia from the starting strain by shake-flask culture; performing ARTP mutagenesis on the mycelia, performing coating onto a LiCl-contained culture medium panel, and performing culturing; pre-screening the picked single colony blocks with a panel, and transferring thepicked single colonies with relatively large inhibition zone diameters onto a checking slope; and performing shake flask checking on the checking slope, and selecting strains with high content of Zhongshengmycin. According to the method, the strains with high content of Zhongshengmycin are screened, fermentation liquor of the strains has an excellent effect for preventing rice bacterial leaf blight, cabbage soft rot, apple ring rot, apple leaf spot and the like, and can quickly screen out the strains with high content of Zhongshengmycin.

The invention relates to a compound fermentation technology and a formula of a compound amino acid purine mother liquor. Fermentation microbes belong to the group of Actinomycetes, and are Streptomyces microflavus, Streptomyces flaveolus and Streptomyces lavendulae. The mother liquor comprises, by weight: 0.1 to 30% of compound amino acid, 0.1 to 30mg / g of oxyenadenine, and 0.1 to 30mg / g of enadenine.

The invention discloses streptomyces lavendulae of which the classification is named as Streptomyces lavendulae UN-8, the preservation number is CCTCC NO: M 2015512, the preservation date is September 6th, 2015, and the preservation unit is China Center for Type Culture Collection. The Streptomyces lavendulae UN-8 has application in the aspects of fermenting and preparing alpha- glucosidase inhibitor. A certain amount of fermentation liquor of the alpha-glucosidase inhibitor is accumulated through strain fermentation, bactofugation is conducted, soluble starch, protein and part pigment are removed through ultrafiltration, decoloration is conducted through activated carbon, nanofiltration concentration is conducted, most inorganic salt micromolecules are removed, adsorption and desorption are conducted through resin, decompression drying is conducted, and an alpha-glucosidase inhibitor refined product is obtained. The alpha-glucosidase inhibitor can strongly inhibit alpha-glucosidase, and IC50 of the alpha-glucosidase inhibitor is 321 times that of commercially available alpha-glucosidase inhibitor acarbose; the obvious improvement action on forming of postprandial hyperglycemia inside an animal is achieved, the effect is equal to that of the acarbose, and the alpha-glucosidase inhibitor can be used for preparing drugs for treating diabetes or functional foods.

The invention discloses a tobacco microbial fertilizer. The tobacco microbial fertilizer comprises four strains, namely Saccharomyces cerevisiae, Streptomyces lavendulae, Bacillus amyloliquefaciens and Trichoderma viride. of the tobacco microbial fertilizer. The tobacco microbial fertilizer is prepared from the following steps: preparing the following four strains; The invention further disclosesa method for preparing a decomposed solid fermentation agent. According to the microbial agent provided by the invention, the synergistic effect among the four bacteria is utilized in the tobacco microbial fertilizer to quickly form dominant flora, and the dominant flora is matched with sophora alopecuroide straw and other raw materials to fully decompose the raw materials and promote the exertionof the fertilizer efficiency. Meanwhile, the problem that a microbial fertilizer cannot give consideration to fertilizer efficiency and disease and pest resistance is solved. The prepared microbial fertilizer can significantly promote the growth of tobacco, has significant control effects on tobacco bacterial wilt, tobacco black shank, tobacco mosaic disease, root-knot nematode disease and myzuspersicae, has good ecological benefits and economic benefits, and has huge development potential.

The invention discloses a method using streptomyces lavendulae fermentation to produce alpha-glucosidase inhibitors. The method is characterized in that strain activation, seed fermentation broth preparation and liquid fermentation are performed on the strains of Streptomyces lavendulae UN-8 with the preservation number being No. M2015512 to obtain fermentation broth rich in alpha-glucosidase inhibitors. The method has the advantages that the method can widely use cheap carbon and nitrogen sources such as soluble starch, cassava starch, corn starch, dried corn steep liquor powder and inorganic ammonium salt; the method adopting liquid-submerged fermentation is convenient to operate and implement, free of pollution, high in production efficiency and capable of satisfying the requirements of industrial production; preliminary identification shows that the alpha-glucosidase inhibitors produced by the method are novel alpha-glucosidase inhibitors, the alpha-glucosidase inhibitors can intensely inhibit alpha-glucosidase, and the IC50 of the alpha-glucosidase inhibitors is 321 times of that of the commercially available alpha-glucosidase inhibitor, namely acarbose; in-vivo experiments show that the alpha-glucosidase inhibitors can evidently improve the formation of postprandial hyperglycemia, the effect of the alpha-glucosidase inhibitors is equivalent to that of the acarbose, and the alpha-glucosidase inhibitors can be used for preventing and treating diabetes.

The invention provides a microbial pesticide for preventing and controlling tea pests and diseases and an application thereof. The pesticide is cultivated by bacillus subtilis, trichoderma, bacillus thuringiensis, streptomyces lavendulae, Wuyimycin, bean dregs and sucrose water. The microbial pesticide has a wide range of preventing pests and diseases, has a significant effect on the prevention and control of tea diseases, and meets the production requirements of green pollution-free tea.

The invention discloses application of streptomyces lavendulae to preventing and treating dry rot of actinidia arguta and relates to the field of the agricultural biotechnology. The streptomyces lavendulae is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.9972. Sterile fermentation filtrate 100-fold diluent of the streptomyces lavendulae is directly sprayed to the stems of the actinidia arguta or poured to the roots of the actinidia arguta, and the prevention effect on dry rot of the actinidia arguta can reach 88.6%. The process is simple, cost is low, use is convenient, use of chemical pesticide in the cultivation process of the actinidia arguta can be reduced, the advantages of being safe and environmentally friendly are achieved, and good application and development prospects are achieved.

The present invention belongs to the technical field of biological pesticides, discloses a Streptomyces lavendulae xjy which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The present invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by the xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

The invention provides streptomyces lavendulae S3-5 and application thereof to preventing and treating corn gray leaf spots. The preservation number of the streptomyces lavendulae S3-5 is CGMCCNo.11236, the bacterial strain, fermentation liquid of the bacterial strain, antibacterial matter of the bacterial strain, and seed processing agents prepared through the fermentation liquid of the bacterial strain are used for preventing and treating corn gray leaf spots caused by cercospora zeae-maydis (C. zeae-maydis), the preventive effect is high, industrialized production can be achieved, the preparation technology is simple, the using method is simple, and streptomyces lavendulae S3-5 is free of toxin and pollution and environmentally friendly.

Application of a strain of Streptomyces lilacinus 2-1-2F-1 and its antibacterial substances in the prevention and control of vegetable soil-borne diseases, preservation number CGMCC No.19768, the advantages and effects of the present invention: a stable source of natural raw materials, bacterial strains It comes from soil, and the screening method is simple; the raw materials of the fermentation medium, such as soybean meal powder and glucose, have low cost; the preparation of antibacterial active substances is simple and the operation is convenient;

The invention discloses a bio-organic fertilizer, as well as a preparation method and application thereof, and belongs to the field of preparation of bio-organic fertilizers. The invention discloses the bio-organic fertilizer at first, which is prepared from the following components: an organic matter, a lactobacillus acidophilus agent, a Streptomyces lavendulae agent and a bacillus mucilaginosus agent. The invention further discloses the preparation method for the bio-organic fertilizer. The method comprises the following steps: crushing the organic matter, uniformly mixing the crushed organic matter, the lactobacillus acidophilus agent, the Streptomyces lavendulae agent and the bacillus mucilaginosus agent, and performing drying. According to the bio-organic fertilizer, each agent is reasonably matched, and an obvious synergistic effect is achieved among the agents, so that the soil fertility can be effectively improved, the crop yield can be increased, and particularly, a remarkable yield increasing effect is achieved when the bio-organic fertilizer is applied to soybean planting.

The invention relates to a Streptomyces lavendulae strain, which is named as Streptomyces lavendulae in classification. The Streptomyces lavendulae strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.7548. The invention also relates to application of the Streptomyces lavendulae to preparation of algae microbial fertilizer. The Streptomyces lavendulae strain provided by the invention has a prominent degrading effect on algae residues; waste residues generated after seaweed gel is extracted by the algae processing industry can be recycled, and antibiotic metabolic active substances generated in the fermenting process of the Streptomyces lavendulae can effectively inhibit growth of various pathogenic microorganisms; the Streptomyces lavendulae has excellent effects of promoting growth and increasing yield and has an obvious effect in agricultural production.

The invention provides Streptomyces lavendulae 2-1-2F-1 and application of the biomass of the Streptomyces lavendulae 2-1-2F-1 in prevention and treatment of soil-borne diseases of vegetables, wherein the preservation number of the Streptomyces lavendulae 2-1-2F-1 is CGMCC No: 19768. According to the invention, the Streptomyces lavendulae 2-1-2F-1 has stable natural raw material sources, the strain comes from soil, and the screening method is simple; culture medium raw materials used for fermentation, such as soybean cake powder and glucose, are low in cost; the antibacterial active substance is simple to prepare and convenient to operate; and antibiotic substances come from the nature, are green and environmentally friendly, and have a good effect of preventing and treating soil-borne diseases of vegetables.

The invention discloses a novel antibacterial biological dye. The biological dye is prepared by culturing microorganisms, taking sodium glutamate or / and glutamine or / and an approximate compound as a substrate, and separating and purifying a culture to obtain a dark blue substance, namely, the biological dye, wherein the microorganisms are one or more of Photorhabdus Luminescens, Streptomyces Lavendulae and Erwinia Chrysanthemi, or engineering bacteria with the same effect; the dark blue substance is a bicyclo-3,3'-bipyridine molecule, and has typical carbonyl structure characteristics. The biological dye is cultured and separated from the microorganisms, has relatively high natural activity, is more environmental-friendly, and is insoluble in water; a water-soluble compound is formed underthe action of a reducing agent under an alkaline condition; fabric is dyed by dip dyeing or pad dyeing, and the like; and the biological dye has a good dyeing effect on natural fibers such as cottonfabric.

The invention discloses a streptomyces lavendulae Hainan variant strain and a method for preparing a zhongshengmycin product by using the streptomyces lavendulae Hainan variant strain. The preservation number of the streptomyces lavendulatus var. Hainanensis strain is as follows: the preservation number of the streptomyces lavendulatus var. Hainanensis strain is GDMCC (China General Microbiological Culture Collection Center) No.61906, and the preservation number of the streptomyces lavendulatus var. Hainanensis strain is as follows: GDMCC No.61906. When the strain obtained by the invention is applied to the industrial production of the zhongshengmycin, the industrial fermentation production level of the zhongshengmycin can be greatly improved by more than 50% and can be up to more than 18000 [mu] / ml, so that a large amount of fully water-soluble zhongshengmycin concentrate dry powder products with the zhongshengmycin content of more than 50000 [mu] / g can be obtained; the industrial production cost of the zhongshengmycin is greatly reduced, a foundation is laid for the market demand of the biopesticide zhongshengmycin, and the extended use in the fields of green ecological agriculture large-range large-scale flight prevention pesticide and the like is met.

The invention discloses a mycelium or thallus, application of the mycelium or thallus and a method for breeding a bacterial strain by using the mycelium or thallus. The mycelia or thalli are obtained from fermentation liquor at the tail section of a secondary metabolite production rapid growth and accumulation stage in a strain fermentation production process. When the obtained mycelium or thallus is applied to breeding of a streptomyces lavendulae strain resistant to metabolite, a Zhongshengmycin high-yield strain is screened, the industrial fermentation production level of the high-yield strain can be greatly improved, the industrial production cost of Zhongshengmycin is greatly reduced, and the method is suitable for industrial production of Zhongshengmycin. A foundation is laid for the market demand of Zhongshengmycin in biopesticide, and the extended use in the fields of green ecological agriculture large-range large-scale flight prevention pesticide and the like is met.

The invention relates to an electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery. The electromagnetic coupling fermentation Streptomyces lavendulae var. hainanensis new var. variant microorganism biological battery comprises an anode carbon rod, a cathode carbon rod, a shake flask culture medium, a fixed-value resistor, a variable resistor, a proton exchange membrane, a wastewater configuration liquid, an external electric field, a workstation, a pH sensor, a dissolved oxygen sensor, a stirrer, an oxygenation device and an external magnetic field, wherein the anode carbon rod is inoculated with the Streptomyces lavendulae var. hainanensis new var. variant. By adopting the electromagnetic coupling environment, the production titer of the Streptomyces lavendulae can be improved, and meanwhile, wastewater generated in the fermentation production process of biopesticide can be degraded.

The invention relates to a microbial agent as well as a preparation method and application thereof, and belongs to the field of comprehensive prevention and control of plant diseases. The microbial agent is prepared from functional microorganism, a nutritional agent and a protective agent, wherein the microorganism is prepared from streptomyces jingyangensis, streptomyces lavendulae, streptomycesaureofaciens and viridin. The functional microorganism of the microbial agent is screened from the natural world; the effect is achieved in a way of inhibiting bacteria by the bacteria, pathogenic bacteria is killed, environmental pollution is avoided and the using of medicines is reduced; the microbial agent is safe and harmless, a harmful influence to crops and a human body is avoided, and the microbial agent can be applied to spraying of plants, soaking of seeds, spray coating of seedlings, disinfection of irrigation water, disinfection of farm tools, soil treatment and the like; a route oftransmission of the pathogenic bacteria can be blocked in many ways so as to prevent and control the diseases; a using method of the microbial agent is flexible, safe and easy.

The invention discloses an actinomycete for preventing and treating peanut diseases and its application. The strain LX14 is named as Streptomyces lavendulae. Deposit unit: General Microbiology Center of China Microbiological Culture Collection Management Committee, deposit date: 2014 On January 22, the deposit number was CGMCCNo.8806. The present invention separates and screens the metabolite Streptomyces lavendulae from soil, which has a strong inhibitory effect on peanut fungi, and is applied to the prevention and treatment of peanut crown rot, peanut scab, peanut root rot and peanut white silkworm. The prevention and control of peanut diseases is of great significance, and it plays a very important role in the biological control of plant diseases. It adds a new way to the development of agricultural fungicides and has a good development and application prospect.