Method for inoculation by adopting streptomyces aureofaciens freezing shake flask hypha to replace spore

A technology of Streptomyces aureus and mycelium, applied in the field of microorganisms, can solve the problems of increasing personnel, equipment investment requirements, unfavorable production cost control of enterprises, etc., achieves the effects of convenient cultivation and quality control, improvement of production resistance, and reduction of labor intensity

Inactive Publication Date: 2013-05-01
ZHUMADIAN HUAZHONG CHIA TAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This undoubtedly increases the input requirements of personnel and equipment, which is not conducive to the production cost control of enterprises, and also brings pressure to the preservation and management of bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In the chlortetracycline fermentation process, the current chlortetracycline fermentation seed inoculation process is to prepare spore liquid from 3-6 well-grown spore bottles, and then use the differential pressure method to transfer the spore liquid into the seed tank for cultivation. After the seed tanks are cultivated, they are transferred to fermenters for fermentation. During the preparation of spore liquid, a large number of spore vials are needed to meet the needs of production, and the preservation of a large number of spore vials requires multiple refrigeration equipment.

[0016] This project focuses on the development of a new inoculation process (shaking flask mycelia inoculation) to reduce the amount of spores used and improve the resistance and work efficiency of Streptomyces aureus. Specifically: use a well-grown spore bottle, and use the method of digging to get 1cm 2 Left and right spores were inserted into sterilized shake flasks for culture. After ...

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Abstract

The present invention relates to a method for inoculation by adopting streptomyces aureofaciens freezing shake flask hypha to replace spore. The method comprises: (1) spore culture, (2) shake flask hypha culture, (3) seed culture, and (4) deep liquid ventilation fermentation. According to the present invention, a new inoculation process (shake flask hypha fermentation) is emphatically developed, the freezing shake flask hypha is adopted to replace the spore to be inoculated so as to reduce a use amount of the spore, improve consistency of a fermentation seed age and seed quality, stabilize microbial metabolism, reduce seed preparation workload and labor intensity, and improve resistance production capacity and work efficiency of streptomyces aureofaciens.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a method for inoculating with frozen shake flask mycelia of Streptomyces aureus instead of spores. [0002] In the chlortetracycline fermentation process, the current chlortetracycline fermentation seed inoculation process is to prepare spore liquid from 3-6 well-grown spore bottles, and then use the differential pressure method to transfer the spore liquid into the seed tank for cultivation. During the preparation of spore liquid, a large number of spore bottles need to be prepared to meet the needs of production, and the preservation of a large number of spore bottles requires multiple refrigeration equipment. [0003] This patent focuses on the development of a new inoculation process (shaking flask mycelium into the tank) to reduce the amount of spores used. Improve the resistance production ability and work efficiency of Streptomyces aureus. Specifically: use a well-grown spo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/49
Inventor 李亚峰王友琦张雪峥
Owner ZHUMADIAN HUAZHONG CHIA TAI
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